Tumor-Suppressive miR-192-5p Has Prognostic Value in Pancreatic Ductal Adenocarcinoma

Flammang, Isabelle; Reese, Moritz; Yang, Zixuan; Eble, Johannes A.; Dhayat, Sameer

doi:10.3390/cancers12061693

Cited by 30 publications

(22 citation statements)

References 55 publications

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“…In the present context, expression of miR-181a-2-3p, miR-216b-5p and miR-214-5p were significantly different in PDAC tissue compared to CP tissue by both NGS and qRT-PCR but there was no significant difference in their serum levels between the two groups of patients. This observation is not unique to this study as many other reports show little or no concordance between tissue and circulatory levels of the same microRNA 24,40 . This indicates that due care should be given to determine the miRNA levels in both tissue and serum as miRNAs showing concordance would serve as better biomarkers.…”

Section: Discussionmentioning

confidence: 50%

“…Likewise, controversial results have been reported about the alteration of miR-192-5p in PDAC tissue specimens. While Flammang et al 40 and Botla et al 41 reported lower expression of miR-192-5p in PDAC tissue; others have reported increased expression in pancreatic cancer tissue 24,42,43 . In fact, ectopic expression of miR-192-5p associates with enhanced cell proliferation and migration, reduced apoptosis and promotes cell cycle progression in pancreatic cancer 43 .…”

Section: Discussionmentioning

confidence: 95%

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Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma

Khan

Singh

Rashid

et al. 2021

Sci Rep

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Early-stage diagnosis of pancreatic ductal adenocarcinoma (PDAC) is difficult due to non-specific symptoms. Circulating miRNAs in body fluids have been emerging as potential non-invasive biomarkers for diagnosis of many cancers. Thus, this study aimed to assess a panel of miRNAs for their ability to differentiate PDAC from chronic pancreatitis (CP), a benign inflammatory condition of the pancreas. Next-generation sequencing was performed to identify miRNAs present in 60 FFPE tissue samples (27 PDAC, 23 CP and 10 normal pancreatic tissues). Four up-regulated miRNAs (miR-215-5p, miR-122-5p, miR-192-5p, and miR-181a-2-3p) and four down-regulated miRNAs (miR-30b-5p, miR-216b-5p, miR-320b, and miR-214-5p) in PDAC compared to CP were selected based on next-generation sequencing results. The levels of these 8 differentially expressed miRNAs were measured by qRT-PCR in 125 serum samples (50 PDAC, 50 CP, and 25 healthy controls (HC)). The results showed significant upregulation of miR-215-5p, miR-122-5p, and miR-192-5p in PDAC serum samples. In contrast, levels of miR-30b-5p and miR-320b were significantly lower in PDAC as compared to CP and HC. ROC analysis showed that these 5 miRNAs can distinguish PDAC from both CP and HC. Hence, this panel can serve as a non-invasive biomarker for the early detection of PDAC.

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 95%

Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma

Khan

Singh

Rashid

et al. 2021

Sci Rep

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“…29 miR-192-5p downregulation plays a tumor-inhibitor part and probably acts a diagnostic and prognostic biomarker in pancreatic ductal adenocarcinoma. 30 Curcumin inhibited cell viability, induced apoptosis, and inhibited the PI3K/Akt signaling pathway through the overexpression of miR-192-5p in human non-small-cell lung cancer. 31 miR-192 is upregulated in cervical cancer, precancerous tissue, and serum compared with the normal tissue, so miR-192 could be regarded as potential biomarker for early diagnosis of cervical cancer.…”

Section: Trpm7 Is a Direct Target Gene Of Mir-192-5pmentioning

confidence: 98%

Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer

Dong

Zhuang

Wang

et al. 2021

The Kaohsiung J of Med Scie

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Cervical cancer is the fourth highest mortality cancer among women worldwide.Many researchers have discovered the major anticancer role of miR-192-5p.However, no study has revealed the effect of miR-192-5p on cervical cancer and its molecular mechanism. Therefore, in this study, we aimed to explore the role of miR-192-5p in proliferation, invasion of cervical cancer, and its regulatory mechanism. Firstly, the expression level of miR-192-5p was examined by real-time quantitative polymerase chain reaction. Cell counting kit-8 analysis was applied to detect the proliferation of transfected Caski and SiHa cells. Flow cytometry assay was applied to detect the apoptosis of transfected Caski and SiHa cells. Our result showed that miR-192-5p restrained cervical cancer cell proliferation and induced apoptosis. Then we employed wound healing and transwell assays to analyze the migration and invasion abilities of Caski and SiHa cells in vitro. The results showed that miR-192-5p had an inhibitory effect on cervical cancer migration and invasion. The results of in vivo experiment demonstrated that miR-192-5p also inhibited tumor development in nude mice. We further detected that the binding of transient receptor potential melastatin-subfamily member 7 (TRPM7) to miR-192-5p using bioinformatic methods and dual-luciferase reporter assay. Finally, we found that TRPM7 overexpression reversed the inhibitory effects of miR-192-5p on proliferation, migration, and invasion on cervical cancer cells. In conclusion, the findings of the present study revealed that miR-192-5p performs an inhibitory role in cervical cancer proliferation and invasion by targeting TRPM7.

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“…In addition to proteins also sEV-resident miRNAs were investigated as a potential diagnostic tool for the early detection of PDAC. Serum-derived sEVs from PDAC patients were reported to contain elevated levels of miR-192-5p, miR-19a-3p, and miR-19b-3p, when compared to healthy controls [ 195 , 196 ]. Additional studies described significantly more miR-10b, miR-17-5p and miR-21 in PDAC patient samples [ 197 , 198 , 199 , 200 ].…”

Section: Sevs As Biomarkers For Prognosis and Predictionmentioning

confidence: 99%

Pancreatic Cancer Small Extracellular Vesicles (Exosomes): A Tale of Short- and Long-Distance Communication

Waldenmaier¹,

Seibold²,

Seufferlein³

et al. 2021

Cancers

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Even with all recent advances in cancer therapy, pancreatic cancer still has a dismal 5-year survival rate of less than 7%. The most prevalent tumor subtype is pancreatic ductal adenocarcinoma (PDAC). PDACs display an extensive crosstalk with their tumor microenvironment (TME), e.g., pancreatic stellate cells, but also immune cells to regulate tumor growth, immune evasion, and metastasis. In addition to crosstalk in the local TME, PDACs were shown to induce the formation of pre-metastatic niches in different organs. Recent advances have attributed many of these interactions to intercellular communication by small extracellular vesicles (sEVs, exosomes). These nanovesicles are derived of endo-lysosomal structures (multivesicular bodies) with a size range of 30–150 nm. sEVs carry various bioactive cargos, such as proteins, lipids, DNA, mRNA, or miRNAs and act in an autocrine or paracrine fashion to educate recipient cells. In addition to tumor formation, progression, and metastasis, sEVs were described as potent biomarker platforms for diagnosis and prognosis of PDAC. Advances in sEV engineering have further indicated that sEVs might once be used as effective drug carriers. Thus, extensive sEV-based communication and applications as platform for biomarker analysis or vehicles for treatment suggest a major impact of sEVs in future PDAC research.

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Tumor-Suppressive miR-192-5p Has Prognostic Value in Pancreatic Ductal Adenocarcinoma

Cited by 30 publications

References 55 publications

Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma

Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma

Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer

Pancreatic Cancer Small Extracellular Vesicles (Exosomes): A Tale of Short- and Long-Distance Communication

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