2013
DOI: 10.1126/science.1227299
|View full text |Cite
|
Sign up to set email alerts
|

Tunable Signal Processing Through Modular Control of Transcription Factor Translocation

Abstract: Signaling pathways can induce different dynamics of transcription factor (TF) activation. We explored how TFs process signaling inputs to generate diverse dynamic responses. The budding yeast general stress responsive TF Msn2 acted as a tunable signal processor that could track, filter, or integrate signals in an input dependent manner. This tunable signal processing appears to originate from dual regulation of both nuclear import and export by phosphorylation, as mutants with one form of regulation sustained … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

3
162
2

Year Published

2014
2014
2023
2023

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 141 publications
(167 citation statements)
references
References 33 publications
3
162
2
Order By: Relevance
“…Recently, new biological sensors have been developed that translocate between the nucleus and the cytoplasm in response to a stimulus. These molecules typically maintain high signal-to-noise ratios, provide robust readouts in response to changes in signaling activity and are generally easier to use than FRET reporters (Hao et al, 2013;Regot et al, 2014;Spencer et al, 2013). Here, we have developed and characterized a translocation reporter for Akt kinase activity based on the transcription factor FoxO1.…”
Section: Development Of a Translocation Reporter For Akt Activitymentioning
confidence: 99%
“…Recently, new biological sensors have been developed that translocate between the nucleus and the cytoplasm in response to a stimulus. These molecules typically maintain high signal-to-noise ratios, provide robust readouts in response to changes in signaling activity and are generally easier to use than FRET reporters (Hao et al, 2013;Regot et al, 2014;Spencer et al, 2013). Here, we have developed and characterized a translocation reporter for Akt kinase activity based on the transcription factor FoxO1.…”
Section: Development Of a Translocation Reporter For Akt Activitymentioning
confidence: 99%
“…Changes in the subcellular localization of signaling proteins as a consequence of signal progression offer the opportunity to observe signaling as it occurs. In the yeast Saccharomyces cerevisiae, signaling has been monitored employing cytoplasmic-nuclear shuttling of protein kinases and transcription factors for the osmo-sensing High Osmolarity Glycerol (HOG) pathway (the Hog1 MAP kinase) (1)(2)(3)(4)(5), the calcineurin pathway (the Crz1 transcriptional activator) (6), the protein kinase A pathway (the Msn2 transcriptional activator) (7)(8)(9), and oxidative stress signaling through Yap1 (10). Several interesting system properties have been observed, including perfect adaptation of the Hog1 signaling system (1), slowdown of protein movements as a consequence of molecular crowding in compressed cells following osmostress (3,5), the potential benefits of nuclear accumulation bursts to coordinate multigene responses to external signals or for the survival of cell populations (8,9), and how coupling of dynamic phenomena may establish a rate of change sensor (7).…”
mentioning
confidence: 99%
“…This has been very recently described for yeast Msn2, a transcription factor responding to general stress and capable of filtering different stress inputs to generate graded gene expression outputs (13). Furthermore, among the often numerous genes activated in response to a given stress, the cell has to impose different sensitivities to guarantee an equilibrated adaptive response.…”
mentioning
confidence: 99%