Two-dimensional countercurrent chromatography × high performance liquid chromatography with heart-cutting and stop-and-go techniques for preparative isolation of coumarin derivatives from Peucedanum praeruptorum Dunn

Liu, Jinglan; Wang, Xinyuan; Zhang, Lingling; Fang, Meijuan; Wu, Yue; Wu, Zhixian; Qiu, Ying-Kun

doi:10.1016/j.chroma.2014.11.053

Cited by 36 publications

(17 citation statements)

References 26 publications

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“…This interface allows the complete elimination of the 1 D mobile phase, the focusing of the solutes on the trapping columns and the transfer to HPLC with compatible injection solvent. Four publications have been published in 2014 and 2015 using CCC devices and preparative LC [65][66][67][68]. The interface of the first application of Qiu's group in 2014 [65] is presented in Figure 11.…”

Section: -Sample Loop Interfacementioning

confidence: 99%

“…This strategy was also applied in 2015 with an instrumental improvement on the water addition before trapping, through the use of a fragmentary dilution and turbulent mixing interface [68]. In 2014 and 2015, Qiu's group illustrated their development through two new 2D LLC-HPLC separation examples [66,67]. For these applications, the same instrumentation was enforced with the same two trapping columns.…”

Section: -Sample Loop Interfacementioning

confidence: 99%

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Preparative two dimensional separations involving liquid–liquid chromatography

Marlot

Faure

2017

Journal of Chromatography A

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The increasing performances of analytical techniques, especially two-dimensional liquid chromatography hyphenated with highly sensitive detectors, allow discovery of new targeted compounds in highly complex samples, whether biomarkers in environmental effluents, natural metabolomes in natural products or unknown impurities in synthetic chemical process. While structural identification can usually be achieved through mass spectrometry and databases, it can be useful to confirm the potential structure via NMR analysis and/or to obtain standard reference material for quantitative purposes, incentive for the production of μg to mg of new target compounds. Hence, preparative chromatography, which was initially run for large-scale production, is now facing new challenges, with small volumes of highly complex samples to deal with. Unfortunately, one dimensional chromatographic methods exhibit limited performances when targets are minor compounds in a complex matrix. Moreover, for process intensification or limited amount of sample, it is now a priority to be able to isolate multiple components with the largest yield possible using as few purification steps as possible. With these specifications in mind, a comprehensive multidimensional chromatographic method for preparative purposes is becoming appealing.

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Section: -Sample Loop Interfacementioning

confidence: 99%

Section: -Sample Loop Interfacementioning

confidence: 99%

Preparative two dimensional separations involving liquid–liquid chromatography

Marlot

Faure

2017

Journal of Chromatography A

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“…products including chemical components, peptides and polymers [1][2][3][4][5][6]. It has been widely used for preparative separation of effective components from natural products.…”

Section: Introductionmentioning

confidence: 99%

“…Combination of CCC and HPLC, also named as 2D chromatography, provides an even more robust and versatile methods for separation of complex matrix, which has been increasingly explored in separation of different natural Article Related Abbreviations: CCC, countercurrent chromatography; 1 D, first dimension; 2 D, second dimension. products including chemical components, peptides and polymers [1][2][3][4][5][6]. On-line comprehensive 2D chromatography has been conventionally applied in gas chromatography (GC × GC) and liquid chromatography (LC×LC) and they have been well studied because of their powerful separation efficiency for complex samples [7,8].…”

Section: Introductionmentioning

confidence: 99%

Off‐line comprehensive two‐dimensional reversed‐phase countercurrent chromatography with high‐performance liquid chromatography: Orthogonality in separation of Polygonum cuspidatum Sieb. et Zucc

Wang

Zhao

Wang

et al. 2019

J of Separation Science

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Off-line comprehensive two-dimensional reversed-phase countercurrent chromatography with high-performance liquid chromatography was investigated in separation of crude ethanol extract from traditional Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc. Two-dimensional contour plots for countercurrent chromatography with high-performance liquid chromatography was obtained after comprehensive separation was completed. Total peak capacity was evaluated and approximately 810 peaks were obtained through a comprehensive two-dimensional separation. A highly orthogonality of 52.23% and a large separation space occupancy of 88.86% were achieved.Meanwhile, it was found that several components could be well separated by countercurrent chromatography while they could not be separated by high-performance liquid chromatography, and vice versa, which further indicated the orthogonality of the two separation methods. The off-line comprehensive two-dimensional countercurrent chromatography with high-performance liquid chromatography provided a promising and powerful method for separation of complex natural products. K E Y W O R D Scountercurrent chromatography, comprehensive two-dimensional chromatography, high performance liquid chromatography, orthogonality, traditional Chinese medicine

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“…The procedure of this off‐line HSCCC mode is complicated and inconvenient. Some online 2D, even multi‐dimensional (multi‐D), HSCCC strategies combined with recycling mode were applied in the isolation potential of natural products, which are usually combined with more HSCCC systems or with high performance liquid chromatography (HPLC), solid phase extraction (SPE), and so on (Lu et al , ; Lu et al , ; Lu et al , ; Liu et al , ; Liu et al , ). Such 2D and multi‐D HSCCC strategies have higher equipment requirements and lead to greater experimental costs.…”

Section: Introductionmentioning

confidence: 99%