Two-dimensional small-angle scattering from single particles in infrared with a lensless technique

Abstract

An experiment is described where two-dimensional small-angle light scattering (2D-SALS) patterns from single particles are measured in the infrared through a lens-free approach. Spatial filtering is employed to separate scattered light from unscattered light to within approximately one degree from the forward direction. Non-planar reflective elements are used in the filtering process, permitting 2D-SALS measurements to be done without chromatic aberrations over a broad spectral range and from 0.8 to 8 degrees in the polar scattering angle and zero to 360 degrees in the azimuthal angle. Patterns from spherical microparticles are presented along with nonspherical particles including volcanic ash and salt. An asymmetry analysis is applied to demonstrate an ability to differentiate spherical from nonspherical particles from the 2D-SALS patterns.

Identification of refractive index for label-free single cells based on 2D light scattering technology

“…Two dimensional (2D) light scattering technology has been applied to the identification and classification of single cells. By analyzing 2D light scattering patterns, more internal information of cells can be obtained [3][4][5][6][7][8][9][10] . Su et al have explored the rich information of the 2D light scattering patterns by employing the anisotropic cytometry for label-free single cell analysis 3 .…”