Two genomic species in Borrelia burgdorferi

Postić, D.; Edlinger, C.; Richaud, Catherine; Grimont, Francine; Dufresne, Yoann; Perolat, P.; Baranton, Guy; Grimont, Patrick A. D.

doi:10.1016/0923-2508(90)90072-x

Cited by 104 publications

(95 citation statements)

References 32 publications

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“…In addition, rDNA restriction patterns were determined with Southern blot analyses, and the data were compared with the genomic grouping scheme of Postic et al (15). RNA isolation and sequencing.…”

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confidence: 99%

“…By using the polymerase chain reaction, differences in the reactivity of various isolates with several different chromosomally derived primers have been observed (16). Differing restriction patterns of the genes coding for rRNA (rDNA) have recently been observed with Southern blot analysis (15). At the protein level, there appear to be significant differences in the reactivities of particular isolates with monoclonal antibodies (4,22).…”

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confidence: 99%

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Phylogenetic analysis of the genus Borrelia: a comparison of North American and European isolates of Borrelia burgdorferi

Marconi

Garon

1992

J Bacteriol

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We have sequenced the 16S rRNA molecules from four species of Borrelia and from six isolates of Borrelia burgdorferi via the reverse transcriptase primer extension method. The sequences were aligned and evolutionary relationships were determined, including the calculation of evolutionary distances and the construction of a phylogenetic tree. These analyses demonstrate significant divergence among B. burgdorferi isolates, with the European isolates Gl and G2 residing most distant from the main cluster. Signature nucleotides which distinguish B. burgdo,feri from all other members of this genus and which distinguish the European isolates Gl and G2 from the North American isolates B31, Sh-2-82, and 1352 were identified. Finally, Southern blot analyses were performed to compare the restriction patterns of the genes coding for rRNA and to relate our data to the grouping scheme of Postic et al.

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confidence: 99%

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Phylogenetic analysis of the genus Borrelia: a comparison of North American and European isolates of Borrelia burgdorferi

Marconi

Garon

1992

J Bacteriol

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“…Borrelia burgdorferi, the Lyme disease spirochaete, has been analysed at the phylogenetic level using DNA-DNA hybridization (Postic et al, 1990), 16s rRNA sequencing (Marconi & Garon, 1992a, b) and multilocus enzyme electrophoresis (Boerlin et al, 1992). Such methods are complex and time-consuming and, by comparison, analysis of FAME profiles is a simpler technique when used in conjunction with the MIDI microbial identification system.…”

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confidence: 99%

Comparison of the fatty acid profiles of Borrelia, Serpulina and Leptospira species

Livesley¹,

Thompson²,

Bailey³

et al. 1993

Journal of General Microbiology

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Fatty acid methyl ester (FAME) derivatives were examined as a means of characterizing Borrelia burgdorferi isolates and distinguishing them from other spirochaetes. Analysis was performed using a gas liquid chromatography column in conjunction with Microbial Identification System (MIS) software. Reproducible FAME profiles were produced which distinguished Borrelia species, Serpulina hyodysenteriae and Leptospira ictevohaemorrhagiae. Furthermore, the FAME profiles of four recognized Borrelia species (including two American isolates of Borrelia burgdorferi, B31 and JDI) were distinct from one another and from the BSK I1 medium in which they were grown. The results confirm previous reports that FAME profiles of bacteria represent a diagnostic phenotypic property and suggest that they may have applications in the chemotaxonomic classification of Borrelia species.

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“…These organisms have been characterized by plasmid profile (8), restriction endonuclease pattern (17,18,26), polymerase chain reaction (PCR) analysis (19,27), immunological analysis and protein profile (30,31). Recently, on the basis of DNA homology (4,26), rRNA and flagellin gene restriction analyses (1), three genotypes for the strains of B. burgdorferi sensu lato have been proposed (4). Two of them are named B. burgdorferi sensu stricto and B. garinii (4,26) and the third genotype is referred to as "group VS461" (4,19).…”

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Genomic Analysis of Borrelia japonica Sp. Nov. Isolated from Ixodes ovatus in Japan

Kawabata

Masuzawa

Yanagihara

1993

Microbiology and Immunology

188

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Genetic characteristics of 12 Borrelia isolates from the tick, Ixodes ovatus, I. persulcatus, and the rodent, Apodemus speciosus ainu, in Japan were compared to members of the three genospecies of Borrelia burgdorferi sensu lato; B. burgdorferi sensu stricto, B. garinii and group VS461. The methods used in this study were the quantitative microplate DNA hybridization assay and restriction fragment length polymorphism (RFLP) analyses of the flagellin structural genes and the 16S rRNA genes. The six isolates from I. persulcatus and A. speciosus ainu were identified as genospecies B. garinii using RFLP analysis of the flagellin and 16S rRNA genes. In contrast, RFLP analysis of the six isolates from I. ovatus indicated that they were different from the three reported genospecies. DNA homology studies confirmed the RFLP results. The six isolates from I. ovatus had DNA homologies ranging from 85 to 99%, whereas DNA relatedness of the I. ovatus isolate with strains belonging to the three genospecies was 50 to 64%. These results suggest that the strains isolated from I. ovatus in Japan differ from the three genospecies and should be classified as a new genospecies of B. burgdorferi sensu lato. We propose that strains isolated from I. ovatus should be classified as B. japonica sp. nov.

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Two genomic species in Borrelia burgdorferi

Cited by 104 publications

References 32 publications

Phylogenetic analysis of the genus Borrelia: a comparison of North American and European isolates of Borrelia burgdorferi

Phylogenetic analysis of the genus Borrelia: a comparison of North American and European isolates of Borrelia burgdorferi

Comparison of the fatty acid profiles of Borrelia, Serpulina and Leptospira species

Genomic Analysis of Borrelia japonica Sp. Nov. Isolated from Ixodes ovatus in Japan

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