Two glycoproteins are produced from the rotavirus neutralization gene

Chan, Wai-Kit; Penaranda, Maria E.; Crawford, Sue E.; Estes, Mary K.

doi:10.1016/0042-6822(86)90046-2

Cited by 63 publications

(34 citation statements)

References 23 publications

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“…Our observation that BTV-1SA $9 encodes two overlapping proteins produced by translation of the same ORF initiating at two distinct AUG codons (VP6 and VP6a) therefore is not unique, but fits into a well established pattern. It also closely resembles the situation observed in another member of the Reoviridae, simian rotavirus SAIl, $9 of which encodes two species of VP7 by using two in-phase initiation codons (Chan et al, 1986).…”

Section: Isa I0supporting

confidence: 70%

“…As is the case with the rotavirus VP7 doublet (Chan et al, 1986), both VP6 and VP6a are packaged within the BTV particle (Mertens et al, 1984), indicating either a requirement for both forms or a recognition of both polypeptides during particle assembly owing to the similarity of the sequences. Recently published data on the genome coding assignment of two other members of the orbivirus genus, African horsesickness virus (Grubman & Lewis, 1992) and epizootic haemorrhagic disease virus (Mecham & Dean, 1988), indicate that a protein doublet is encoded by $9 of these two isolates.…”

Section: Isa I0mentioning

confidence: 91%

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Sequence of genome segment 9 of bluetongue virus (serotype 1, South Africa) and expression analysis demonstrating that different forms of VP6 are derived from initiation of protein synthesis at two distinct sites

Wade-Evans

Mertens

Belsham

1992

Journal of General Virology

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Section: Isa I0supporting

confidence: 70%

Section: Isa I0mentioning

confidence: 91%

Sequence of genome segment 9 of bluetongue virus (serotype 1, South Africa) and expression analysis demonstrating that different forms of VP6 are derived from initiation of protein synthesis at two distinct sites

Wade-Evans

Mertens

Belsham

1992

Journal of General Virology

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“…Capsid protein heterogeneity has been reported with rotaviruses (Chan et al, 1986) and reoviruses (Jayasuriya et al, 1988;Wiener & Joklik, 1988), both of which are members of the Reoviridae family. The use of the second, as well as the first, in-phase AUG codon is attributed to the rotavirus capsid protein heterogeneity (Chan et al, 1986).…”

mentioning

confidence: 99%

Outer capsid protein heterogeneity of rice dwarf phytoreovirus

Suzuki¹,

Sugawara²

1991

Journal of General Virology

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The 46K outer capsid protein encoded by RNA segment $8 and the 42K polypeptide, previously thought to be the segment S9-encoded structural protein, were isolated from a rice dwarf phytoreovirus purified preparation, and then analysed by peptide mapping and electroblot-ELISA. Staphylococcus aureus V8 protease peptide mapping patterns of the 42K and 46K proteins were similar. Two monoclonal antibodies (MAbs), obtained after immunization with virus particles dissociated by 0.1% SDS, were each specific for both the 42K and 46K proteins. Furthermore, the MAbs bound common peptide fragments which were generated by digestion of the 42K and 46K proteins with V8 protease or proteinase K. These results strongly suggest that the 42K protein is not a gene product of $9 but a product overlapping with the 46K outer capsid protein. Whether the two proteins are functionally distinct remains to be determined.Rice dwarf phytoreovirus (RDV) (Boccardo & Milne, 1984) is considered to contain a genome composed of t2 segmented dsRNAs and seven structural proteins. The genomic RNAs are termed S1 to Sl2 in increasing order of mobility in a polyacrylamide gel. It was reported by Nakata et al. (1978) that the seven constituent proteins were encoded by S1, $2, $3, $5, $7, $8 and $9 based both on their potential coding capacities as monocistronic mRNAs and on the sizes of the polypeptides detected in the virus particles.

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“…3a, lane 2) even when the gel concentration was increased, although there was an ORF (bases 449 to 835) on the S11 minus strand. The two products appeared to be translated from the first and second AUG codons, just as in the case of the simian rotavirus SA11 genome segment 9 (Chan et al, 1986). In order to examine this, we obtained a transcript lacking the first AUG using site-directed mutagenesis and in vitro transcription, and subjected it to in vitro translation.…”

Section: S11 Product Assignmentmentioning

confidence: 99%