Two-layer multiplexed peristaltic pumps for high-density integrated microfluidics

Cole, Matthew; Desai, Amit; Kenis, Paul J. A.

doi:10.1016/j.snb.2010.07.012

Cited by 18 publications

(14 citation statements)

References 27 publications

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“…For the devices characterized below, all of the channels were 50-m wide and 17-20-m deep. Previously, flow rates have been shown to depend upon several factors such as actuation pressure, actuation frequency, dimensions of the fluidic channels, effective area of the on-off valves, and gap between pumps [21,33]. As expected, the flow rate increased as actuation pressure increased ( Fig.…”

Section: Device Characterizationsupporting

confidence: 58%

High‐throughput microfluidic device for single cell analysis using multiple integrated soft lithographic pumps

et al. 2016

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The ability to accurately control fluid transport in microfluidic devices is key for developing high-throughput methods for single cell analysis. Making small, reproducible changes to flow rates, however, to optimize lysis and injection using pumps external to the microfluidic device are challenging and time-consuming. To improve the throughput and increase the number of cells analyzed, we have integrated previously reported micropumps into a microfluidic device that can increase the cell analysis rate to ∼1000 cells/h and operate for over an hour continuously. In order to increase the flow rates sufficiently to handle cells at a higher throughput, three sets of pumps were multiplexed. These pumps are simple, low-cost, durable, easy to fabricate, and biocompatible. They provide precise control of the flow rate up to 9.2 nL/s. These devices were used to automatically transport, lyse, and electrophoretically separate T-Lymphocyte cells loaded with Oregon green and 6-carboxyfluorescein. Peak overlap statistics predicted the number of fully resolved single-cell electropherograms seen. In addition, there was no change in the average fluorescent dye peak areas indicating that the cells remained intact and the dyes did not leak out of the cells over the 1 h analysis time. The cell lysate peak area distribution followed that expected of an asynchronous steady-state population of immortalized cells.

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Section: Device Characterizationsupporting

confidence: 58%

High‐throughput microfluidic device for single cell analysis using multiple integrated soft lithographic pumps

et al. 2016

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“…Peristaltic pumping transfers fluid by serial actuation of this membrane; 235 peristaltic pumps integrated in a PDMS-glass device were used for labeling, dilution and separation of amino acids in an automated manner. 236 Cole et al 235 reported a method for controlling multiple peristaltic pumps using fewer external connections. Other designs of pneumatic micropumps involving actuation by a single control line or serpentine-shape pneumatic channels are discussed by Lai and Folch, 237 and Huang et al, 238 respectively.…”

Section: Functions In Lab-on-a-chip Systemsmentioning

confidence: 99%

Advances in Microfluidic Materials, Functions, Integration, and Applications

2013

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“…An example is given by Cole et al and is depicted at the righthand side of Figure 7.2 [17]. Combining the anisotropic etching of silicon and thin-film technology with electrical control circuitry and a means of a voltage applied to the actuator element; electrostatics will regulate the distance between the silicon boss structure and the microvalve seat.…”

Section: Valving By Micromechanical Actuationmentioning

confidence: 99%

“…(Reproduced from reference[17]. Left: schematic of an electrostatically actuated silicon valve enabled by anisotropic wet etching.…”

mentioning

confidence: 99%

Microfluidic Components, Devices and Integrated Lab-on-a-Chip Systems

Lüttge¹

2011

Microfabrication for Industrial Applications

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Two-layer multiplexed peristaltic pumps for high-density integrated microfluidics

Cited by 18 publications

References 27 publications

High‐throughput microfluidic device for single cell analysis using multiple integrated soft lithographic pumps

High‐throughput microfluidic device for single cell analysis using multiple integrated soft lithographic pumps

Advances in Microfluidic Materials, Functions, Integration, and Applications

Microfluidic Components, Devices and Integrated Lab-on-a-Chip Systems

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