Two Main Features of T-Cell Development: Thymus Traffic and Postthymic Maturation

Stutman, Osias

doi:10.1007/978-1-4684-3054-7_1

Cited by 70 publications

(72 citation statements)

References 110 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). Taken together, these results indicate that the nu/ nu mice studied were conventional in H-2 type and devoid of measurable T-cell immunocompetence (3,4,(38)(39)(40)(41)(42).…”

Section: Resultsmentioning

confidence: 57%

See 1 more Smart Citation

Restriction specificities, alloreactivity, and allotolerance expressed by T cells from nude mice reconstituted with H-2-compatible or -incompatible thymus grafts.

Zinkernagel¹,

Althage²,

Waterfield³

et al. 1980

The Journal of Experimental Medicine

133

View full text Add to dashboard Cite

Congenitally thymusless nude mice that lacked functional T cells were reconstituted with H-2-compatible or -incompatible thymus grafts taken from either fetal, newborn, or adult mice and transplanted under the kidney capsule or subcutaneously. Transplantation with unirradiated fetal (15--17 d) or newborn thymus grafts reconstituted the nude mice as assessed by their subsequent generation of virus-specific cytotoxic T cells in vivo or alloreactive T cells in vitro. The restriction specificity of T cells from homozygous mice was exclusively for the nude host H-2, as shown by direct cytolysis or by cold target competitive inhibition assays. irrespective of whether nude mice were reconstituted with H-2-compatible, semiallogeneic, or H-2-incompatible, unirradiated newborn or fetal thymus grafts (in order of decreasing efficiency of reconstitution). The restriction specificity for the nonhost H-2 of the thymus could not be demonstrated even after primary or secondary sensitization in an infected appropriate F1 environment. These nude mice reconstituted with fetal or newborn grafts were tolerant to the H-2 of the thymus donors. Nude mice transplanted with irradiated adult thymus grafts were reconstituted functionally with syngeneic or semisyngeneic but not with allogeneic thymus grafts. In homozygous nu/nu irradiated heterozygous recipients of F1 thymus grafts, the restriction specificity for the nonhost thymic H-2 could not be elicited upon adoptive sensitization in irradiated and infected F1 heterozygote stimulator mice; in fact, these chimeras' lymphocytes were not tolerant to the nonhost H-2. The discrepancy between the restorative capacity of unirradiated vs. irradiated thymus grafts suggests that precursors of T cells in nude mice can acquire restriction specificity and immunocompetence independently of a conventional, functioning H-2-compatible thymus if exposed to an allogeneic fetal or a newborn thymus that contains functioning thymocytes of donor type but not if reconstituted with an irradiated adult allogeneic thymus.

show abstract

“…2). Taken together, these results indicate that the nu/ nu mice studied were conventional in H-2 type and devoid of measurable T-cell immunocompetence (3,4,(38)(39)(40)(41)(42).…”

Section: Resultsmentioning

confidence: 57%

“…The literature contains few studies assessing T-cell function in nude mice reconstituted with irradiated allogeneic thymus grafts (3,4,(40)(41)(42). In general, these studies agree that reconstitution is limited at best.…”

Section: Discussionmentioning

confidence: 97%

Restriction specificities, alloreactivity, and allotolerance expressed by T cells from nude mice reconstituted with H-2-compatible or -incompatible thymus grafts.

Zinkernagel¹,

Althage²,

Waterfield³

et al. 1980

The Journal of Experimental Medicine

133

View full text Add to dashboard Cite

show abstract

“…In some cases, as in certain patients with severe combined immunodeficiency, the absence of an effect was taken as confirmation of the assumption that there was an absence of prethymic lymphoid precursor cells. Another possible reason for a failure of thymic factors in individuals with a complete absence of the thymus is suggested by the work of Stutman [86] indicating that thymic humoral factors act predominately on post-thymic cells, that is cells that have undergone partial differentiation following interaction with thymic epithelium. Thus thymic humoral factors may not reconstitute an effective T cell immune response in patients with a complete absence of the thymus.…”

Section: Hnplications Jbr the Therapymentioning

confidence: 99%

T cell disorders in primary immunodeficiency diseases

Waldmann

Broder

1978

Springer Semin Immunopathol

View full text Add to dashboard Cite

“…The need for such clarification is enhanced by experimental evidence suggesting that a proportion of functional T cells arises in the periphery from postthymic T cell precursors that are discharged from the thymus in an immature state and complete their differentiation outside the thymus, generating immunocompetent T lymphocytes (Stutman, 1977(Stutman, , 1978Ching and Miller, 1982). Could some of the T cell precursors in the bone marrow, in fact, belong to this postthymic T cell precursor population?…”

Section: Introductionmentioning

confidence: 96%

The role of hematogenous and intrinsic precursor cells in lymphocyte production in murine bone marrow and thymus

et al. 1991

View full text Add to dashboard Cite

It is well recognized that the bone marrow contains cells that can repopulate a depleted thymus as well as cells that can be induced to express phenotypic markers characteristic of T cells. It is not known, however, to what extent thymocytopoiesis in the normal thymus relies on immigrant, bone marrow-derived cells, nor whether some T cell precursors have entered the bone marrow from the circulation. We used the parabiotic system to test whether thymocytopoiesis relies on progenitors intrinsic to the thymus or on cells that enter the organ from the circulation. In the same system, we have also investigated whether Thy-1- bone marrow lymphocytes that respond to phytohemagglutinin (PHA) by proliferation and Thy-1 expression are produced by myelogenous or hematogenous progenitors. Syngeneic CBA/HT6 and CBA/CaJ mice were joined in parabiotic union at 4-6 weeks of age. Cross circulation between the two partners was verified by the equilibration of Evans' blue dye injected into one partner and by the equilibration of PHA-responsive T cells in the spleen of the parabionts. Chromosome spreads were prepared from the PHA-stimulated T cell-depleted bone marrow and from spontaneously proliferating thymocytes as well as from thymocytes stimulated by PHA or Concanavalin A (Con A). The exchange of spleen colony-forming units (CFU-S) in the femoral marrow was assessed by karyotyping individual spleen colonies. Regardless of the length of parabiotic union, ranging from 4 to 20 weeks, Thy-1-, PHA-responsive bond marrow lymphocytes remained predominantly of the host type with only 3% being derived from the opposite partner.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Two Main Features of T-Cell Development: Thymus Traffic and Postthymic Maturation

Cited by 70 publications

References 110 publications

Restriction specificities, alloreactivity, and allotolerance expressed by T cells from nude mice reconstituted with H-2-compatible or -incompatible thymus grafts.

Restriction specificities, alloreactivity, and allotolerance expressed by T cells from nude mice reconstituted with H-2-compatible or -incompatible thymus grafts.

T cell disorders in primary immunodeficiency diseases

The role of hematogenous and intrinsic precursor cells in lymphocyte production in murine bone marrow and thymus

Contact Info

Product

Resources

About