Two new species of Neosartorya from Amazonian soil, Brazil

Horie, Yoshikazu; Abliz, Paride; Fukushima, Kazutaka; Okada, Kaoru; Campos-Takaki, Galba Maria de

doi:10.1007/s10267-003-0132-1

Cited by 18 publications

(8 citation statements)

References 12 publications

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“…The majority of species in the Trichocomaceae family are homothallic, with eight confirmed exceptions. Six of the exceptions belong to the genus Neosartorya (N. fennelliae, N.spathulata, N. nishimurae, N. udagawae, N. indohii, and N. tsurutae [16,17,25,46,48]), one belongs to the genus Emericella (E. heterothallica [36]), and Talaromyces derxii belongs to Penicillium subgenus Biverticillium (47).…”

Section: Discussionmentioning

confidence: 99%

Sexual Reproduction as the Cause of Heat Resistance in the Food Spoilage Fungus Byssochlamys spectabilis (Anamorph Paecilomyces variotii )

Houbraken

Varga

Rico-Munoz³

et al. 2008

Appl Environ Microbiol

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Paecilomyces variotii is a common cosmopolitan species that is able to spoil various food-and feedstuffs and is frequently encountered in heat-treated products. However, isolates from heat-treated products rarely form ascospores. In this study we examined by using molecular techniques and mating tests whether this species can undergo a sexual cycle and form ascospores. The population structure of this species was examined by analyzing the nuclear ribosomal internal transcribed spacer 1 (ITS1) and ITS2 and the 5.8S rRNA gene, as well as partial ␤-tubulin, actin, and calmodulin gene sequences. Phylogenetic analyses revealed that P. variotii is a highly variable species. Partition homogeneity tests revealed that P. variotii has a recombining population structure. In addition to sequence analyses, mating experiments indicated that P. variotii is able to form ascomata and ascospores in culture in a heterothallic manner. The distribution of MAT1-1 and MAT1-2 genes showed a 1:1 ratio in the progeny of the mating experiments. From the sequence analyses and mating data we conclude that P. variotii is the anamorph of Talaromyces spectabilis and that it has a biallelic heterothallic mating system. Since Paecilomyces sensu stricto anamorphs group within Byssochlamys, a new combination Byssochlamys spectabilis is proposed. (2) is a common cosmopolitan mold found in soils, indoor environments, plants, animals, and foodstuffs (33,39,40,42). It is a fast-growing thermotolerant fungus that is able to grow at low oxygen levels and in the presence of preservatives, able to form the mycotoxin viriditoxin, and able to survive heat treatments (19,33). The combination of the previously mentioned features makes this species an important organism and one of the most frequently encountered spoilage fungi in various foodstuffs. This species is one of the most heat-resistant fungi known, and its presence in pasteurized beverages causes great economic losses (52). Although P. variotii produces thick-walled hyphae and chlamydospores, ascospores, which are usually considered to be the heat-resistant propagule in other fungi, are rarely found in culture. As a result, the route of P. variotii contamination remains mostly unknown (19). Besides spoiling food, it is also associated with many types of human infections. Endocarditis is the most common infection produced by this species and has a very bad prognosis (5). Industrial applications have also been proposed with P. variotii isolates; examples include tannase production (3, 28) and biofiltration of toluene (7, 10). Paecilomyces variotii BainierAscomycetes with anamorphs in the genera Penicillium, Aspergillus, and Paecilomyces have been classified in the Trichocomaceae family (2, 29, 30, 34). The connection of Paecilomyces anamorphs with the ascomycete Byssochlamys was first proposed by Stolk and Samson (44). A recent molecular study revealed that P. variotii forms a well-defined clade within the Trichocomaceae, together with other thermophilic Paecilomyces species and Byssochlamys teleomorph...

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Section: Discussionmentioning

confidence: 99%

Sexual Reproduction as the Cause of Heat Resistance in the Food Spoilage Fungus Byssochlamys spectabilis (Anamorph Paecilomyces variotii )

Houbraken

Varga

Rico-Munoz³

et al. 2008

Appl Environ Microbiol

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“…Ascomycetous fungi living in soil are relatively unstudied and which may be a potential source of novel natural products for utilization in medicine, pharmaceutical products, agriculture and industry [2]. The fungal genus Neosartorya belongs to phylum Ascomycota which has a worldwide distribution in the soil [3]. This research focused on the isolation of Neosartorya spp.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Ascomycetous Fungi, Neosartorya spp. and Screening for Its Antibacterial Metabolites

Sanmanoch¹,

Mongkolthanaruk²,

Kanokmedhakul³

et al. 2013

JOLST

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 Abstract-The aim of the present study was to identify ascomycetous fungi (Neosartorya spp.) and examined the antibacterial activities of crude mycelium extracted by ethyl acetate from these fungal genera. Among 10 ascomycetous fungal isolates, Neosartorya sp., were screened from soil and showed a broader antimicrobial spectrum than the other. Especially, fungal strain KKU-1NK1 showed strongly antibacterial activities. This fungal strain was belonging to Neosartorya spinosa which was identified using 5.8S rRNA gene sequencing analysis. The results revealed that the growth of clinical isolates of gram-positive pathogenic bacteria (Methicillin-resistant Staphylococcus aureus DMST 20654, Staphylococcus aureus ATCC 25923, Staphylococcus saprophyticus ATCC 15305, Streptococcus pneumonia DMST 15319 and Bacillus subtilis ATCC 6633) was well inhibited by crude mycelial extracted from N. spinosa KKU-1NK1 at concentrations values of 10 mg/ml. Moreover, the effects of submerge culture media variation on mycelia growth which affected to total production of bioactive compounds and antibacterial activity by N. spinosa KKU-1NK1 was observed.

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“…2 ) and could be easily microscopically differentiated from any other Neosartorya species (Samson et al 1990 ; Horie et al. 2003 ). Both isolates produced gliotoxin, while CBS 652.73 also produced viriditoxin.…”

Section: Resultsmentioning

confidence: 99%

New taxa of Neosartorya and Aspergillus in Aspergillus section Fumigati

Hong

Shin

Hong³

et al. 2007

Antonie van Leeuwenhoek

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Three new species of Neosartorya and one new Aspergillus of section Fumigati are proposed using a polyphasic approach based on morphology, extrolite production and partial b-tubulin, calmodulin, and actin gene sequences. The phylogenetic analyses using the three genes clearly show that the taxa grouped separately from the known species and confirmed the phenotypic differences. Neosartorya denticulata is characterized by its unique denticulate ascospores with a prominent equatorial furrow; N. assulata by well developed flaps on the convex surface of the ascospores which in addition have two distinct equatorial crests and N. galapagensis by a funiculose colony morphology, short and narrow conidiophores and ascospores with two wide equatorial crests with a microtuberculate convex surface. Aspergillus turcosus can be distinguished by velvety, gray turquoise colonies and short, loosely columnar conidial heads. The four new taxa also have unique extrolite profiles, which contain the mycotoxins gliotoxin and viriditoxin in N. denticulate; apolar compounds provisionally named NEPS in N. assulata and gregatins in N. galapagensis. A. turcosus produced kotanins. N. denticulata sp. nov., N. assulata sp. nov., N. galapagensis sp. nov., and A. turcosus sp. nov. are described and illustrated.

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Two new species of Neosartorya from Amazonian soil, Brazil

Cited by 18 publications

References 12 publications

Sexual Reproduction as the Cause of Heat Resistance in the Food Spoilage Fungus Byssochlamys spectabilis (Anamorph Paecilomyces variotii )

Sexual Reproduction as the Cause of Heat Resistance in the Food Spoilage Fungus Byssochlamys spectabilis (Anamorph Paecilomyces variotii )

Isolation of Ascomycetous Fungi, Neosartorya spp. and Screening for Its Antibacterial Metabolites

New taxa of Neosartorya and Aspergillus in Aspergillus section Fumigati

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