Two novel serum biomarkers for endometriosis screened by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry and their change after laparoscopic removal of endometriosis

Proteomics Analysis of Plasma for Early Diagnosis of Endometriosis

“…In our study, the total number of patients included was much higher (nϭ254) than in previous reports (median 87, range 32-141; Table 4). Our study population was also well characterized with respect to menstrual cycle phase, whereas previous studies did not include any cycle phase description 8,9,11,12,14 or did not confirm cycle phase descrip-…”

“…24 -26 Based on our pilot study, we chose Proteominer kit to deplete highly abundant proteins from plasma and to enrich the surfaceenhanced laser desorption/ionization time-of-flight mass spectrometry spectra. Other investigators depleted plasma or serum samples using a U9 buffer solution (9 mm/L urea; 2% CHAPS; 50 mM Tris HCl, pH 9.0) 8,9,14 or CHAPS combined with Cibacron Blue 3GA. 11,12 However, they never reported if these methods could enrich the peak spectra after surfaceenhanced laser desorption/ionization time-of-flight mass spectrometry analysis.…”

Urinary peptide profiling identifies a panel of putative biomarkers for diagnosing and staging endometriosis

“…However, the majority of the markers identified with this method are either of low sensitivity and specificity, their positive predictive value is not accurate enough to be used for routine clinical purposes, or even their molecular nature is not determined. These studies have focused primarily on blood and tissues (i.e., endometrial, endometriotic, peritoneal) to analyze proteomes in endometriosis (30)(31)(32)(33)(34)(35)(36)(37).…”

The diagnosis of endometriosis with the help of mass spectrometry (a review)

“…[80] с по-мощью метода SELDI-TOF MS сообщается о созда-нии высокочувствительного алгоритма диагностики эндометриоза (чувствительность 87%, специфич-ность 97%) по определению двух пептидных пиков с молекулярной массой 5830 и 8,865 Д в сыворотке крови. В этом исследовании сопоставили уровень СА-125 в крови для сравнительного анализа.…”