Two-stage thermophilic bio-hydrogen and methane production from oil palm trunk hydrolysate using Thermoanaerobacterium thermosaccharolyticum KKU19

Sitthikitpanya, Saruda; Reungsang, Alissara; Prasertsan, Poonsuk; Khanal, Samir Kumar

doi:10.1016/j.ijhydene.2017.09.136

Cited by 27 publications

(6 citation statements)

References 73 publications

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“…The results indicate that the use of enzyme loading at 15 FPU/g-sugarcane leaves was sufficient to degrade the cellulose structure. Moreover, a low loading of enzymes is preferable over high loading because it lowers the operational costs [ 2 , 21 ]. Thus, the optimum conditions for alkaline pretreatment and enzymatic hydrolysis of sugarcane leaves were 2% (w/v) NaOH concentration and 15 FPU/g-sugarcane leaves.…”

Section: Resultsmentioning

confidence: 99%

“…After alkali pretreatment, the solid fraction was filtered through a muslin cloth, rinsed with tap water until pH 7–8, and then dried at 80 °C for 24 h in a hot air oven. Subsequently, 2.5 g of pretreated sugarcane leaves were hydrolyzed with Cellic ® CTec2 (Novozyme) at a 35 FPU/g-sugarcane leaves in 50 mL of 0.05 M sodium citrate buffer (pH 5.0) [ 2 ] and incubated in a shaking water bath at 50 °C and 150 rpm for 72 h. The TRS concentrations of sugarcane leaf hydrolysate were analyzed using the 3,5-dinitrosalicylic acid (DNS) method. The conditions that gave the highest TRS concentration were further used to optimize the enzyme loadings of 5, 15, 25, 35, 45, 55, and 65 FPU/g-sugarcane leaves.…”

Section: Methodsmentioning

confidence: 99%

“…Biohydrogen is a renewable energy resource that has received attention because of its sustainability, clean combustion product, and high energy content of 122 kJ/g, which is 2.5 times that of fossil fuels [ 1 ]. Biohydrogen is widely produced by dark fermentation due to ease of operation and low operation cost, a high production rate, and commercialization potential [ 2 ]. Recently, microalgal biomass has been used to produce biohydrogen by dark fermentation [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

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Co-generation of biohydrogen and biochemicals from co-digestion of Chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept

Sitthikitpanya

Sittijunda

Khamtib³

et al. 2021

Biotechnol Biofuels

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Background A platform for the utilization of the Chlorella sp. biomass and sugarcane leaves to produce multiple products (biorefinery concept) including hydrogen, methane, polyhydroxyalkanoates (PHAs), lipid, and soil supplement with the goal to achieve the zero waste generation (circular economy) is demonstrated in this study. Microalgal biomass were hydrolyzed by mixed enzymes while sugarcane leaves were pretreated with alkali followed by enzyme. Hydrolysates were used to produce hydrogen and the hydrogenic effluent was used to produce multi-products. Solid residues at the end of hydrogen fermentation and the remaining acidified slurries from methane production were evaluated for the compost properties. Results The maximum hydrogen yield of 207.65 mL-H2/g-volatile solid (VS)added was obtained from 0.92, 15.27, and 3.82 g-VS/L of Chlorella sp. biomass hydrolysate, sugarcane leaf hydrolysate, and anaerobic sludge, respectively. Hydrogenic effluent produced 321.1 mL/g-VS of methane yield, 2.01 g/L PHAs concentration, and 0.20 g/L of lipid concentration. Solid residues and the acidified slurries at the end of the hydrogen and methane production process were proved to have compost properties. Conclusion Hydrogen production followed by methane, PHA and lipid productions is a successful integrated circular biorefinery platform to efficiently utilize the hydrolysates of Chlorella sp. biomass and sugarcane leaf. The potential use of the solid residues at the end of hydrogen fermentation and the remaining acidified slurries from methane production as soil supplements demonstrates the zero waste concept. The approach revealed in this study provides a foundation for the optimal use of feedstock, resulting in zero waste. Graphic Abstract

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Co-generation of biohydrogen and biochemicals from co-digestion of Chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept

Sitthikitpanya

Sittijunda

Khamtib³

et al. 2021

Biotechnol Biofuels

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“…The hydrogen content in the biogas was analyzed using GC (Shimadzu GC-2014, Japan) equipped with a thermal conductivity detector (TCD) and a 2 m stainless steel column packed with Shin carbon (50/80 mesh). The operating conditions were set following the study of Sitthikitpanya et al [40]. The volume of hydrogen produced (mL) was calculated using an equation proposed by Zheng and Yu [41].…”

Section: Methodsmentioning

confidence: 99%

Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)

et al. 2019

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Simultaneous saccharification and fermentation (SSF) and pre-hydrolysis with SSF (PSSF) were used to produce hydrogen from the biomass of Chlorella sp. SSF was conducted using an enzyme mixture consisting of 80 filter paper unit (FPU) g-biomass−1 of cellulase, 92 U g-biomass−1 of amylase, and 120 U g-biomass−1 of glucoamylase at 35 °C for 108 h. This yielded 170 mL-H2 g-volatile-solids−1 (VS), with a productivity of 1.6 mL-H2 g-VS−1 h−1. Pre-hydrolyzing the biomass at 50 °C for 12 h resulted in the production of 1.8 g/L of reducing sugars, leading to a hydrogen yield (HY) of 172 mL-H2 g-VS−1. Using PSSF, the fermentation time was shortened by 36 h in which a productivity of 2.4 mL-H2 g-VS−1 h−1 was attained. To the best of our knowledge, the present study is the first report on the use of SSF and PSSF for hydrogen production from microalgal biomass, and the HY obtained in the study is by far the highest yield reported. Our results indicate that PSSF is a promising process for hydrogen production from microalgal biomass.

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“…Hydrogen content in the biogas was analyzed using gas chromatography (GC) following the method of Sitthikitpanya et al (2017) . The values of the kinetic parameters for hydrogen production were estimated using the modified Gompertz equation as decribed by Khanal et al (2004) .…”

Section: Methodsmentioning

confidence: 99%

Improvement of hydrogen production from Chlorella sp. biomass by acid-thermal pretreatment

Giang

Lunprom

Liu

et al. 2019

PeerJ

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Background Owing to the high growth rate, high protein and carbohydrate contents, and an ability to grow autotrophically, microalgal biomass is regarded as a promising feedstock for fermentative hydrogen production. However, the rigid cell wall of microalgae impedes efficient hydrolysis of the biomass, resulting in low availability of assimilable nutrients and, consequently, low hydrogen production. Therefore, pretreatment of the biomass is necessary in order to achieve higher hydrogen yield (HY). In the present study, acid-thermal pretreatment of Chlorella sp. biomass was investigated. Conditions for the pretreatment, as well as those for hydrogen production from the pretreated biomass, were optimized. Acid pretreatment was also conducted for comparison. Results Under optimum conditions (0.75% (v/v) H2SO4, 160 °C, 30 min, and 40 g-biomass/L), acid-thermal pretreatment yielded 151.8 mg-reducing-sugar/g-biomass. This was around 15 times that obtained from the acid pretreatment under optimum conditions (4% (v/v) H2SO4, 150 min, and 40 g-biomass/L). Fermentation of the acid-thermal pretreated biomass gave 1,079 mL-H2/L, with a HY of 54.0 mL-H2/g-volatile-solids (VS), while only 394 mL/L and 26.3 mL-H2/g-VS were obtained from the acid-pretreated biomass. Conclusions Acid-thermal pretreatment was effective in solubilizing the biomass of Chlorella sp. Heat exerted synergistic effect with acid to release nutrients from the biomass. Satisfactory HY obtained with the acid-thermal pretreated biomass demonstrates that this pretreatment method was effective, and that it should be implemented to achieve high HY.

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Two-stage thermophilic bio-hydrogen and methane production from oil palm trunk hydrolysate using Thermoanaerobacterium thermosaccharolyticum KKU19

Cited by 27 publications

References 73 publications

Co-generation of biohydrogen and biochemicals from co-digestion of Chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept

Co-generation of biohydrogen and biochemicals from co-digestion of Chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept

Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)

Improvement of hydrogen production from Chlorella sp. biomass by acid-thermal pretreatment

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