Two-trace two-dimensional (2T2D) correlation infrared spectral analysis of Spirulina platensis and its commercial food products coupled with chemometric analysis

Kavitha, E.; Stephen, L. Devaraj; Brishti, Fatema Hossain; Karthikeyan, S.

doi:10.1016/j.molstruc.2021.130964

Cited by 23 publications

(8 citation statements)

References 52 publications

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“…Performance of measures male population ROC analysis of FTIR spectra helps to diagnose biological samples with high sensitivity and speci city [19,39,40]. It is a statistically valid method for biomarker evaluation.…”

Section: Discussionmentioning

confidence: 99%

Two-trace two-dimensional correlation spectra (2T2D-COS) analysis using FTIR spectra to monitor the immune response by COVID-19

Karthikeyan,

Vazquez-Zapien,

Martinez-Cuazitl

et al. 2023

Preprint

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There is a growing trend in using saliva for SARS-CoV-2 detection with reasonable accuracy. We have studied the responses of IgA, IgG, and IgM in human saliva by directly comparing disease with control analyzing two-trace two-dimensional correlation spectra (2T2D-COS) employing Fourier transform infrared (FTIR) spectra. The findings show the decrease of IgG and IgM salivary antibodies in the 50, 60, 65, and 75-years samples. Among the various COVID-19 populations studied, the female 30 years group reveal defence mechanisms exhibited by IgM and IgA. Lipids and fatty acids decrease, resulting in lipid oxidation due to the SARS-CoV-2 in the samples studied. Study shows salivary thiocyanate plays defence against SARS-CoV-2 in male population in 25 and 35 age groups. The receiver operation characteristics statistical method shows a sensitivity of 98% and specificity 94% for the samples studied. The measure of accuracy computed as F1 score and G score has a high value which supports the validation of our study. The 2T2D-COS analysis has the potential to monitor the progression of immunoglobulin’s response function of COVID-19 with good accuracy, which could help in diagnosing clinical trials.

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Section: Discussionmentioning

confidence: 99%

Two-trace two-dimensional correlation spectra (2T2D-COS) analysis using FTIR spectra to monitor the immune response by COVID-19

Karthikeyan,

Vazquez-Zapien,

Martinez-Cuazitl

et al. 2023

Preprint

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“…The peak at 1,650 cm −1 is attributed to the stretch of C=O from Amide I, and the band at 1,546 cm −1 is assigned to the N-H stretching and C-N bending vibrations from Amide II, so the FTIR spectral region from 1,700-1,500 cm −1 is characterized by the amide bands in proteins. In contrast, the peaks within 1,200-900 cm −1 are mainly associated with C-O-C stretching from polysaccharides (Kavitha et al, 2021;Zhang et al, 2022). It can be seen that the cellular constitutes vary in different algal strains which can be FIGURE PCA results of the carotenoids and lipids region (ca.…”

Section: Preprocessing Methods Evaluationmentioning

confidence: 97%

Screening of Spirulina strains for high copper adsorption capacity through Fourier transform infrared spectroscopy

Liu

Zhu

et al. 2022

Front. Microbiol.

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Microalgae have emerged as promising biosorbents for the removal of toxic metals from industrial effluents due to the presence of various free functional groups. While the constitutes are distinct among different algal strains, it needs to screen the algae with high adsorption capacities for heavy metal ions by analyzing the algal components. In this study, a rapid and nondestructive Fourier transform infrared (FTIR) method combined PCA algorithm was used to discriminate algal strains according to their cellular components. With FTIR spectroscopy, we have found that the algal strains for high copper adsorption capacity (RH44, XS58, AH53, and RZ22) can be well differentiated from other strains via assessing the components involved in the biosorption of copper ions at the spectral window range of 1,200–900 cm−1 mainly attributed to polysaccharides. Correspondingly, the copper removal efficiency by different Spirulina strains was also measured by biochemical assay and scanning electron microscopy (SEM) in order to confirm the screening result. Compared with the chemical measurement, the assessment based on spectral features appears fairly good in the evaluation and differentiation of copper adsorption capacity in various Spirulina strains. This study illustrates that FTIR spectroscopy may serve as a fast and effective tool to investigate the functional groups for copper ions binding in the Spirulina cell and it even offers a useful and accurate new approach to rapidly assess potential adsorbents for the high capacity of copper adsorption.

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“…ROC analysis of FTIR spectra helps to diagnose biological samples with high sensitivity and speci city [19,39,40]. It is a statistically valid method for biomarker evaluation.…”

Section: Performance Of Measures Male Populationmentioning

confidence: 99%

“…The coronavirus disease-2019 (COVID- 19) is an acute infectious respiratory illness caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). The COVID-19 pandemic is a global health issue, affecting more than 220 countries and generating more than 6 million deaths.…”

Section: Introductionmentioning

confidence: 99%

Two-trace two-dimensional correlation spectra (2T2D-COS) analysis using FTIR spectra to monitor the immune response by COVID-19

Karthikeyan,

Vazquez-Zapien,

Martinez-Cuazitl

et al. 2023

J Mol Med

View full text Add to dashboard Cite

There is a growing trend in using saliva for SARS-CoV-2 detection with reasonable accuracy. We have studied the responses of IgA, IgG, and IgM in human saliva by directly comparing disease with control analyzing two-trace two-dimensional correlation spectra (2T2D-COS) employing Fourier transform infrared (FTIR) spectra. The ndings show the decrease of IgG and IgM salivary antibodies in the 50, 60, 65, and 75-years samples. Among the various COVID-19 populations studied, the female 30 years group reveal defence mechanisms exhibited by IgM and IgA. Lipids and fatty acids decrease, resulting in lipid oxidation due to the SARS-CoV-2 in the samples studied. Study shows salivary thiocyanate plays defence against SARS-CoV-2 in male population in 25 and 35 age groups. The receiver operation characteristics statistical method shows a sensitivity of 98% and speci city 94% for the samples studied. The measure of accuracy computed as F1 score and G score has a high value which supports the validation of our study. The 2T2D-COS analysis has the potential to monitor the progression of immunoglobulin's response function of COVID-19 with good accuracy, which could help in diagnosing clinical trials. Receiver operation characteristics (ROC) analysis

show abstract

Two-trace two-dimensional (2T2D) correlation infrared spectral analysis of Spirulina platensis and its commercial food products coupled with chemometric analysis

Cited by 23 publications

References 52 publications

Two-trace two-dimensional correlation spectra (2T2D-COS) analysis using FTIR spectra to monitor the immune response by COVID-19

Two-trace two-dimensional correlation spectra (2T2D-COS) analysis using FTIR spectra to monitor the immune response by COVID-19

Screening of Spirulina strains for high copper adsorption capacity through Fourier transform infrared spectroscopy

Two-trace two-dimensional correlation spectra (2T2D-COS) analysis using FTIR spectra to monitor the immune response by COVID-19

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