Two types of tumor necrosis factor-α in bluefin tuna (Thunnus orientalis) genes: Molecular cloning and expression profile in response to several immunological stimulants

Kadowaki, Takeshi; Harada, Hideaki; Sawada, Yoshifumi; Kohchi, Chie; Soma, Gen‐Ichiro; Takahashi, Yuichiro; Inagawa, Hiroyuki

doi:10.1016/j.fsi.2008.12.006

Cited by 45 publications

(36 citation statements)

References 38 publications

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“…Subfunctionalization is a partitioning event by which paralogues simply undergo a division of labor by retaining different parts (subfunctions) of their original ancestral function. The previously identified TNF-a paralogues are differentially expressed and modulated in tuna (29), orange-spotted grouper (30), and rainbow trout (28). It is now apparent that the three TNF-a paralogues that exist in trout are differentially expressed in different tissues and cell lines (Fig.…”

Section: Differential Expression Of the Three Tnf-a Isoforms In Rainbmentioning

confidence: 87%

“…Multiple isoforms of TNF-a genes have been identified in some fish species [e.g., three TNF-a genes are known in common carp (18,27) and two are known in rainbow trout (28), blue fin tuna Thunnus orientalis (29), and orange-spotted grouper Epinephelus coioides (30)]. The homology between the different isoforms within common carp and rainbow trout (i.e., in the same species) is high: ∼88% identity between the two trout isoforms and 72-78% identity among the three carp TNF molecules (27).…”

mentioning

confidence: 99%

“…Thus, they may have arisen from the recent tetraploidization of the ancestors of trout and carp (27). However, the homology of the TNF-a isoforms identified in blue fin tuna (29) and orange-spotted grouper (30) are much lower, with identities of 43 and 38%, respectively. These isoforms of TNF-a might have arisen from the teleost-wide whole-genome duplication (WGD) event (31), as seen with other fish cytokines, such as M-CSF (32) and IL-1b (33), for which two major forms exist in fish.…”

mentioning

confidence: 99%

“…These isoforms of TNF-a might have arisen from the teleost-wide whole-genome duplication (WGD) event (31), as seen with other fish cytokines, such as M-CSF (32) and IL-1b (33), for which two major forms exist in fish. However, the origin of the tuna and grouper isoforms is unclear, because phylogenetic tree analysis did not support the notion that two types of teleost TNF-a genes may exist as a result of the teleost-wide WGD (29,30).…”

mentioning

confidence: 99%

“…For example, in grouper, one isoform is highly expressed constitutively but is poorly upregulated by immune challenge in vitro and in vivo (29,30). In tuna, grouper, and trout, the TNF-a paralogues are differentially expressed and modulated (28)(29)(30), perhaps suggesting a degree of subfunctionalization (34). The differential expression of the paralogues could allow a fine-tuned regulation of TNF-a-induced cytokines in these species.…”

mentioning

confidence: 99%

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Two Types of TNF-α Exist in Teleost Fish: Phylogeny, Expression, and Bioactivity Analysis of Type-II TNF-α3 in Rainbow Trout Oncorhynchus mykiss

Hong

et al. 2013

The Journal of Immunology

190

160

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TNF-α is a cytokine involved in systemic inflammation and regulation of immune cells. It is produced chiefly by activated macrophages as a membrane or secreted form. In rainbow trout, two TNF-α molecules were described previously. In this article, we report a third TNF-α (TNF-α3) that has only low identities to known trout molecules. Phylogenetic tree and synteny analyses of trout and other fish species suggest that two types (named I and II) of TNF-α exist in teleost fish. The fish type-II TNF-α has a short stalk that may impact on its enzymatic release or restrict it to a membrane-bound form. The constitutive expression of trout TNF-α3 was generally lower than the other two genes in tissues and cell lines, with the exception of the macrophage RTS-11 cell line, in which expression was higher. Expression of all three TNF-α isoforms could be modulated by crude LPS, peptidoglycan, polyinosinic:polycytidylic acid, and rIFN-γ in cell lines and primary macrophages, as well as by bacterial and viral infections. TNF-α3 is the most responsive gene at early time points post-LPS stimulation and can be highly induced by the T cell–stimulant PHA, suggesting it is a particularly important TNF-α isoform. rTNF-α3 produced in CHO cells was bioactive in different cell lines and primary macrophages. In the latter, it induced the expression of proinflammatory cytokines (IL-1β, IL-6, IL-8, IL-17C, and TNF-αs), negative regulators (SOCS1–3, TGF-β1b), antimicrobial peptides (cathelicidin-1 and hepcidin), and the macrophage growth factor IL-34, verifying its key role in the inflammatory cytokine network and macrophage biology of fish.

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Section: Differential Expression Of the Three Tnf-a Isoforms In Rainbmentioning

confidence: 87%