Tympanic membrane wound healing in rats assessed by transcriptome profiling

Abstract

The findings of this study provide a baseline against which to identify disease-related molecular signatures, biomarkers, and to develop new treatments for TM conditions based on molecular evidence.

Analysis of gene expression profiles in tympanic membrane following perforation using PCR Array in rats—Preliminary investigation

“…Also Santa Maria et al [12,27] using the whole rat genome arrays observed increased expression of Hbegf gene. They also observed up-regulation of other growth factors such as: FGF7 (KGF1), FGF2 (bFGF) 12 h after injury [12,28], in the time point that was not included in our study. Ishibashi et al [29,30] also studied the expression of growth factors mRNA during healing of TM perforations using RT-PCR method and found that KGF and TGFa m RNA were induced rapidly, peaking 3 days after wounding and then declining.…”

“…It has been suggested to be the predominant growth factor in the epithelialization by accelerating keratinocyte migration [26]. Also Santa Maria et al [12,27] using the whole rat genome arrays observed increased expression of Hbegf gene. They also observed up-regulation of other growth factors such as: FGF7 (KGF1), FGF2 (bFGF) 12 h after injury [12,28], in the time point that was not included in our study.…”

“…Recently Santa Maria et al [12] published excellent, extensive study on the healing of TM perforations using the whole rat genome microarrays. In our study, only genes known to be involved in the process of the skin healing were examined.…”

Enhanced expression of hepatocyte growth factor in the healing of experimental acute tympanic membrane perforation

“…The literature data concerning the expression of VEGF m RNA in the course of TM healing are inconsistent. In a study of TM wound healing in rats assessed by transcriptome profiling, VEGF was up-regulated at 12 h after injury [13]. The differences in mRNA expression observed in various studies may result from differences in methodological paradigm.…”

“…These differences in TM and skin healing suggest that also growth factors may be expressed differently and that the experience derived from the repair of other tissues may not be fully applicable to TM. Most of the information concerning the role of various biomolecules involved in the coordination of TM healing comes from the research on gene expression [12,13]. Our preliminary study performed using Rat Wound Healing RT 2 Profiler PCR Array showed increased levels of mRNA for hepatocyte (HGF) and vascular endothelial growth factor (VEGFa), mainly in the proliferative stage of the TM healing process [12].…”