The aim of the present study was to examine the relationship of 20 m sprint performance with anthropometrical and physiological parameters in male soccer players. A hundred and 81 soccer players from the region of Athens (age 23.4 ± 5.0 yrs, body mass 73.4 ± 7.7 kg, height 180.0 ± 5.9 cm, body fat (BF) 14.4 ± 3.6%), classified into quartiles according to 20 m sprint time (group A, 2.84-3.03 s; group B, 3.04-3.09 s; group C, 3.10-3.18 s; group D, 3.19-3.61 s), participated. Soccer players in group A were younger and had better performance in vertical jumps and in the Wingate anaerobic test (WAnT, p < 0.05). Sprint time correlated to age (r = 0.27), body mass (r = 0.