Typing Listeria monocytogenes by Random Amplified Polymorphic DNA(RAPD) Fingerprinting.

Yoshida, Tetsuya; Takeuchi, Michiko; Sato, Moritoshi; Hirai, Katsuya

doi:10.1292/jvms.61.857

Cited by 18 publications

(15 citation statements)

References 19 publications

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“…However, our results with RAPD typing showed sharper genomic divisions among serovars. This association between RAPD types and serovars was also detected in L. monocytogenes isolated from different animals and on dairy farms in Japan (29).…”

Section: Discussionsupporting

confidence: 57%

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RAPD‐ and actA Gene‐Typing of Listeria monocytogenes Isolates of Human Listeriosis, the Intestinal Contents of Cows and Beef

Inoue

Katagiri

Terao³

et al. 2001

Microbiology and Immunology

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Seventy-five L monocytogenes isolates of human listeriosis, the intestinal contents of cows and beef were divided into 5 major clusters, 17 sub-clusters and 28 minor clusters by typing using random amplification of polymorphic DNA (RAPD). According to their major RAPD category, L monocytogenes isolates serotyped as 1I2b and 4b were distinguished from L monocytogenes isolates of serovars 1I2a and 1I2c. Moreover serovar 4b was distinguished from serovar 1/2b by a difference in the RAPD sub-cluster category. All L monocytogenes were found to possess either actA gene Type I or IT, and only one actA gene type was detectedin each RAPD minor cluster. actA gene Type ITwas observed in 32.0%, 38.5% and 18.9% of isolates from humans, cows and beef, respectively, and was detected more frequently in serovar 4b (46.9%) than in serovars 1/2a (22.2%), 1I2b (7.7%) and 1/2c (0.0%). Twenty (80%) of 25 human isolates fell within three minor RAPD types (Il-d (16%), V-p-1 (36%), V-p-2 (28%». Two isolates from humans and beef were found to have the same RAPD type (Type IV-k-1), actA gene type (Type I) and serovar (1/2b). Our results suggest that only a few genotypes of L monocytogenes are predominant in human listeriosis in Japan, although the human isolates were collected over a broad span of time and a wide geographical range. Our results also suggest that RAPD-, actA gene-and sero-typing can be useful for epidemiological analysis.

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Section: Discussionsupporting

confidence: 57%

“…L. monocytogenes isolated from raw milk and a bulk tank on a dairy farm were found to share the same random amplified polymorphic DNA (RAPD) type (29). Pulsed-field gel electrophore-*Address correspondence to Dr. Satoshi Inoue, Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan.…”

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confidence: 99%

RAPD‐ and actA Gene‐Typing of Listeria monocytogenes Isolates of Human Listeriosis, the Intestinal Contents of Cows and Beef

Inoue

Katagiri

Terao³

et al. 2001

Microbiology and Immunology

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“…These include (i) PCR-based methods, e.g., randomly amplified polymorphic DNA (19,32) and repetitive sequence-based PCR (18); (ii) restriction digestion-based methods, e.g., ribotyping (RT) (2,26) and pulsed-field gel electrophoresis (PFGE) (1,12,13); and (iii) some other combined amplification-restriction methods, e.g., amplified fragment length polymorphism (14) and PCR-restriction fragment length polymorphism (17). The methods listed above target nucleotide variations at endonuclease restriction or primer annealing sites, utilize the electrophoretic mobility of digested or amplified DNA fragments in agarose gels, and define L. monocytogenes strains by their unique banding patterns.…”

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Multi-Virulence-Locus Sequence Typing ofListeria monocytogenes

Zhang

Jayarao

Knabel

2004

Appl Environ Microbiol

181

146

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A multi-virulence-locus sequence typing (MVLST) scheme was developed for subtyping Listeria monocytogenes, and the results obtained using this scheme were compared to those of pulsed-field gel electrophoresis (PFGE) and the published results of other typing methods, including ribotyping (RT) and multilocus sequence typing (MLST). A set of 28 strains (eight different serotypes and three known genetic lineages) of L. monocytogenes was selected from a strain collection (n > 1,000 strains) to represent the genetic diversity of this species. Internal fragments (ca. 418 to 469 bp) of three virulence genes (prfA, inlB, and inlC) and three virulenceassociated genes (dal, lisR, and clpP) were sequenced and analyzed. Multiple DNA sequence alignment identified 10 (prfA), 19 (inlB), 13 (dal), 10 (lisR), 17 (inlC), and 16 (clpP) allelic types and a total of 28 unique sequence types. Comparison of MVLST with automated EcoRI-RT and PFGE with ApaI enzymatic digestion showed that MVLST was able to differentiate strains that were indistinguishable by RT (13 ribotypes; discrimination index ‫؍‬ 0.921) or PFGE (22 profiles; discrimination index ‫؍‬ 0.970). Comparison of MVLST with housekeeping-gene-based MLST analysis showed that MVLST provided higher discriminatory power for serotype 1/2a and 4b strains than MLST. Cluster analysis based on the intragenic sequences of the selected virulence genes indicated a strain phylogeny closely related to serotypes and genetic lineages. In conclusion, MVLST may improve the discriminatory power of MLST and provide a convenient tool for studying the local epidemiology of L. monocytogenes.

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“…The three serovars (1/2a, 1/2b and 4b), which have frequently been recovered from human and animals with listeriosis, were found to be predominant in the wild animals in Japan. In addition, eight of these 11 L. monocytogenes isolates were typed by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) according to Yoshida et al [18]. Interestingly, two of these 4b isolates showed an identical RAPD profile (type IV-6), in spite of different animal origins, crow and "sika" d e e r. T h i s s u g g e s t s t h a t t h e R A P D t y p e o f L .…”

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confidence: 99%

“…For public health, it is necessary to clarify the epidemiological relationship between L. monocytogenes from wild animals and that from human cases of listeriosis and food. a) The data of RAPD types were retrieved from a previous report [18].…”

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confidence: 99%

Incidence of Listeria monocytogenes in Wild Animals in Japan.

Yoshida¹,

Sugimoto

Sato³

et al. 2000

J. Vet. Med. Sci.

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ABSTRACT. Between 1991 and 1993, the intestinal contents and feces of wild animals in Japan were examined for the presence of Listeria. The wild animals examined included 623 mammals (11 species) and 996 birds (18 species). Listeria species were isolated from 38 (6.1%) of the 623 mammalian samples and 133 (13.4%) of 996 bird samples. The highest incidence of Listeria in the mammals was found in Japanese monkeys (20.0%) and that in birds was found in crows (43.2%). The incidence of Listeria in Japanese monkeys varied from 0 to 40.0% depending on the capture area. L. monocytogenes was isolated from 11 of these positive samples. Serovars 1/2a and 4b predominated in eight serotyped L. monocytogenes isolates.-KEY WORDS: incidence, Listeria, wild animal.Listeria monocytogenes is a gram-positive, rod-shaped and non-spore-forming bacterium [13] and widespread in the environment (i.e., soil, sewage and river water) [1,7,14,16,17]. It is a zoonosis-causative agent pathogenic to many mammalians and bird species [11], and has increasingly been recognized as an important foodborne p a t h o g e n . I n J a p a n , r e p o rt s o n i s o l a t i o n o f L . monocytogenes from wild animals have been limited only to wild rats in urban areas [4][5][6]. In other countries, ho w e v e r, m a n y i n v e s t i g a t i o ns h a ve re p or t e d L . monocytogenes isolation from wild animals other than rats [1,2,10,12,15]. Therefore, it is very important for public health to understand the incidence of Listeria in wild animals in Japan. The purpose of this study was to investigate the incidence of Listeria in wild animals other than wild rats in Japan.During the period from 1991 to 1993, 623 mammals (11 species) and 852 birds (18 species) were captured in 10 prefectures in Japan. Most of them were killed after capture and the intestinal contents were collected. Some crows were caged for a few days before collection of intestinal contents. In addition, fresh feces from 144 rock ptarmigans in Toyama prefecture were collected in Cary-Blair Transport Medium (Nissui, Tokyo) and transported on ice to our laboratory (Table 1).Listeria was isolated by the method of Perry and Donnelly [9]. Namely, each sample was cultured in nine volumes of UVM Modified Listeria Enrichment Broth (Difco, Detroit, MI) at 30°C for 24 hr. Then, 1 ml of each culture was added to 9 ml of modified UVM broth supplemented with acriflavine to a final concentration of 25 mg/l and incubated again at 30°C for 24 hr. After these two-step enrichment, 0.1 ml of the culture was plated on a PALCAM agar (Merck, Darmstadt) plate and incubated at 37°C for 48 hr. Six suspect colonies were picked up from each positive plate and characterized biochemically for Listeria by the method of Seeliger and Jones [13].In mammals, Listeria species were detected from 38 (6.1%) of 623 mammals consisting of seven species, Japanese monkeys (20.0%, 16/80), red foxes (13.3%, 2/15), raccoon dogs (11.5%, 12/104), masked palm civet cats (9.1%, 1/11), Japanese martens (3.9%, 5/127), wild boar (2.7%, 1/3...

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Typing Listeria monocytogenes by Random Amplified Polymorphic DNA(RAPD) Fingerprinting.

Cited by 18 publications

References 19 publications

RAPD‐ and actA Gene‐Typing of Listeria monocytogenes Isolates of Human Listeriosis, the Intestinal Contents of Cows and Beef

RAPD‐ and actA Gene‐Typing of Listeria monocytogenes Isolates of Human Listeriosis, the Intestinal Contents of Cows and Beef

Multi-Virulence-Locus Sequence Typing ofListeria monocytogenes

Incidence of Listeria monocytogenes in Wild Animals in Japan.

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