Typing of Listeria Monocytogenes Isolated from Slaughtered Cattle and Beef Meat

Dmowska, Katarzyna; Wieczorek, Kinga; Lynch, Orla; Osek, Jacek

doi:10.2478/bvip-2013-0033

Cited by 7 publications

(3 citation statements)

References 17 publications

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“…monocytogenes with Listeria pathogenicity island 3 (LIPI-3) [ 21 ], and isolates from the present study exhibited 44.8% (111/248) positivity. This rate is higher than those obtained by Dmowska et al [ 60 ] and Shen et al [ 20 ], who observed that 7.4% and 12.4% of llsX -positive samples from beef and retail meat, respectively. Although all strains isolated from raw meat and quick-frozen foods contained inlA , inlC and inlJ genes, most of these strains (34/49 and 83/108) lacked the llsX gene.…”

Section: Resultscontrasting

confidence: 69%

Listeria monocytogenes Prevalence and Characteristics in Retail Raw Foods in China

Zhang

et al. 2015

PLoS ONE

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The prevalence and levels of Listeria monocytogenes in retail raw foods covering most provincial capitals in China were studied with testing of 1036 samples of vegetables, edible mushrooms, raw meat, aquatic products and quick-frozen products from September 2012 to January 2014. The total prevalence of Listeria monocytogenes was 20.0% (207/1036), and the most probable number (MPN) values of 65.7% of the positive samples ranged from 0.3 to 110 MPN/g. Geographical differences were observed in this survey, and the results of both qualitative and quantitative methods indicated that the levels in the samples from North China were higher than those in the samples from South China. A total of 248 isolates were analyzed, of which approximately half belonged to molecular serogroup 1/2a-3a (45.2%), followed by 1/2b-3b-7 (30.6%), 1/2c-3c (16.1%), 4b-4d-4e (5.2%) and 4a-4c (2.8%). Most of the isolates carried hly (100%), inlB (98.8%), inlA (99.6%), inlC (98.0%) and inlJ (99.2%), and 44.8% of the isolates were llsX-positive. Seventeen epidemic clones (ECs) were detected, with 7 strains belonging to ECI (2.8%) and 10 belonging to ECIII (4.03%). Resistance to clindamycin (46.8%) was commonly observed, and 59 strains (23.8%) were susceptible to all 14 tested antibiotics, whereas 84 (33.9%) showed an intermediate level of resistance or were resistant to two or more antibiotics, including 7 multi-resistant strains that exhibited resistance to more than 10 antibiotics. The data obtained in the present study provides useful information for assessment of the possible risk posed to Chinese consumers, and this information will have a significant public health impact in China. Furthermore, the presence of virulence markers, epidemic clones, as well as the antibiotic resistance amongst the isolates strongly implies that many of these strains might be capable of causing listeriosis, and more accurate treatment of human listeriosis with effective antibiotics should be considered. This research represents a more full-scale and systematical investigation of the prevalence of L. monocytogenes in retail raw foods in China, and it provides baseline information for Chinese regulatory authorities that will aid in the formulation of a regulatory framework for controlling L. monocytogenes with the aim of improving the microbiological safety of raw foods.

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Section: Resultscontrasting

confidence: 69%

Listeria monocytogenes Prevalence and Characteristics in Retail Raw Foods in China

Zhang

et al. 2015

PLoS ONE

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“…The main routes of contamination for animals (especially livestock) are silage (Welchman et al, 1997) and the animals themselves. This animal carriage of L. monocytogenes in the agricultural environment can lead to contamination of animal products such as raw milk (Huntet al, 2012) or meat (Dmowska et al, 2013). Contamination of agricultural raw materials is a pathway for the pathogen to enter the food industry.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, Algeria

Sabrina¹,

Mamache²,

Hamdi³

et al. 2021

American Journal of Animal and Veterinary Sciences

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The aim of this study is the detection of the presence of L. monocytogenes in raw milk in the region of Batna using the real-time polymerase chain reaction (real-time PCR) technique, in order to assess the riskrun by the consumer of raw milk in the study area. 65 milk samples were investigated for the detection of L. monocytogenes. The detection of the hlyA gene in all samples was done by real-time PCR assay by comparative cycle threshold method. The results obtained were negative for all the samples analyzed, therefore the prevalence of L. monocytogenes in the 65 samples collected in this study is 0%. The absence of L. monocytogenes in the samples could be linked to the good health of the selected cattle. In fact, L. monocytogenes is considered to be very widespread in industrialized countries and rare in North Africa and Algeria.

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“…There are many transmission paths of this bacterium among animals, between animals and man, and among people. The main source of infection for animals and humans is contaminated food, predominated by silage for animals and frozen food, meat, fish, unpasteurised milk, and dairy products for people (3,13,14).…”

Section: Introductionmentioning

confidence: 99%

Application of SYBR Green I and TaqMan probe-based real-time PCRs for the identification of Listeria spp. and Listeria monocytogenes

Kędrak-Jabłońska

Budniak

Szczawińska

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes. Five strains of L. monocytogenes and single strains of each of the species:welshimeri, and L. seeligeri were used for the experiments. Additionally, five strains of other species of bacteria were used for evaluation of the specificity of the tests. QuantiTect SYBR Green PCR and QuantiTect Probe PCR kits were selected for the study. In the first stage of the study, SYBR Green I real-time PCRs were performed under several methods, the first one allowing detection of the 23S rDNA gene and the remainder based on the amplification of the hlyA gene. In the next part, three varied in method TaqMan probe-based real-time PCRs allowing confirmation of strains belonging to Listeria spp. and L. monocytogenes were conducted. The observation of amplification curves in real-time PCR methods enabled the detection of both genes, and these methods demonstrated a significant sensitivity and high specificity. A high regression coefficient of 0.99 was found for all reactions. Specific amplification products were obtained for the 23S rDNA and hlyA genes, which confirmed the tested strains as Listeria spp. and L. monocytogenes respectively. Isolates of other microbial species did not yield real-time PCR products.

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Typing of Listeria Monocytogenes Isolated from Slaughtered Cattle and Beef Meat

Cited by 7 publications

References 17 publications

Listeria monocytogenes Prevalence and Characteristics in Retail Raw Foods in China

Listeria monocytogenes Prevalence and Characteristics in Retail Raw Foods in China

Prevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, Algeria

Application of SYBR Green I and TaqMan probe-based real-time PCRs for the identification of Listeria spp. and Listeria monocytogenes

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