Tyrosinase Multilayer-Functionalised Carbon Nanotubes as Electrochemical Labels: Application To Immunoassay

Chumyim, Porramate; Rijiravanich, Patsamon; Somasundrum, Mithran; Surareungchai, Werasak

doi:10.1007/s12668-014-0144-7

Cited by 7 publications

(4 citation statements)

References 45 publications

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“…Table lists the electrochemical labels which have been used for Salmonella immunoassays since 2000 . It can be seen that the platform described here is highly competitive with previous methods, having a lower LOD than all but one than all but one assay (which required a longer time) and being faster than all but one assay (which reported a higher LOD).…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Immunoassay for Salmonella Typhimurium Based on an Immuno‐magnetic Redox Label

et al. 2017

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Abstract:We have described a sensitive voltammetric immunoassay for Salmonella Typhimurium based on the employment of a dual-function label. Methylene blue was adsorbed onto multi-walled carbon nanotubes (MWCNTs) at a loading of 6.5 3 10 À3 mol g À1 , and these modified MWCNTs were then adsorbed onto carboxylic acid-modified Fe 2 O 3 magnetic particles of approx. diameter 1 mm. It was estimated from voltammetry that each magnetic particle delivered 2.3 3 10 8 molecules of methylene blue. Following adsorption of monoclonal antiSalmonella antibodies onto the particles, the particles were used for the immunomagnetic separation of Salmonella cells, which were then detected in a sandwich assay using methylene blue reduction at screen printed electrodes modified with polyclonal anti-Salmonella antibodies. Calibration in buffer and in pasteurized milk (10 to 10 6 CFU mL À1 ) resulted in LODs of 7.9 CFU mL À1 and 17.3 CFU mL À1 respectively, without pre-enrichment. The assay platform showed good discrimination against E. coli in both buffer and milk, and the label fabrication process was shown to be reproducible at a 95 % confidence limit as examined by analysis of variance. When stored at 4 8C the labels gave stable assay responses for a period of 60 days.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Immunoassay for Salmonella Typhimurium Based on an Immuno‐magnetic Redox Label

et al. 2017

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“…The regenerated ortho -quinone can be electrochemically reduced to produce an amplified electrochemical response. For instance, Chumyim developed an electrochemical immunosensor for the detection of Salmonella Typhimurium cells based on tyrosinase multilayer-functionalized CNTs as electrochemical labels and EN redox cycling [ 137 ]. Akanda et al reported integrated electrochemical–chemical–enzymatic (ECN) redox cycling for protein detection [ 138 ].…”

Section: Oxidoreductases As the Signal Labels Of Electrochemical Immu...mentioning

confidence: 99%

Overview on the Development of Electrochemical Immunosensors by the Signal Amplification of Enzyme- or Nanozyme-Based Catalysis Plus Redox Cycling

Xia,

Gao,

Zhang

et al. 2024

Molecules

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Enzyme-linked electrochemical immunosensors have attracted considerable attention for the sensitive and selective detection of various targets in clinical diagnosis, food quality control, and environmental analysis. In order to improve the performances of conventional immunoassays, significant efforts have been made to couple enzyme-linked or nanozyme-based catalysis and redox cycling for signal amplification. The current review summarizes the recent advances in the development of enzyme- or nanozyme-based electrochemical immunosensors with redox cycling for signal amplification. The special features of redox cycling reactions and their synergistic functions in signal amplification are discussed. Additionally, the current challenges and future directions of enzyme- or nanozyme-based electrochemical immunosensors with redox cycling are addressed.

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“…The high and selective catalytic activity of Tyr has therefore led to its frequent application in sensitive biosensors. Such applications include (i) the detection of phenolic − and catechol − compounds, such as phenol and dopamine; (ii) affinity-based detection using Tyr as a catalytic label or signal amplifier; − (iii) measurements of the activities of intracellular enzymes, such as β-galactosidase; − and (iv) measurements of the Tyr activity in tumor cells …”

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confidence: 99%

“…In affinity-based detection, only one Tyr substrate is added to the detection solution despite the fact that Tyr requires two substrates, − and this is possible because dissolved O 2 is already present in the solution. This results in a relatively simple detection procedure.…”

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confidence: 99%

Phenolic Tyrosinase Substrate with a Formal Potential Lower than That of Phenol to Obtain a Sensitive Electrochemical Immunosensor

et al. 2022

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The high and selective catalytic activities of tyrosinase (Tyr) have frequently led to its application in sensitive biosensors. However, in affinity-based biosensors, the use of Tyr as a catalytic label is less common compared to horseradish peroxidase and alkaline phosphatase owing to the fact that phenolic Tyr substrates have yet to be investigated in detail. Herein, four phenolic compounds that have lower formal potentials than phenol were examined for their applicability as Tyr substrates, and three reducing agents were examined as potential strong reducing agents for electrochemical−chemical (EC) redox cycling involving an electrode, a Tyr product, and a reducing agent. The combination of 4methoxyphenol (MP) and ammonia-borane (AB) allows for (i) a high electrochemical signal level owing to rapid EC redox cycling and (ii) a low electrochemical background level owing to the slow oxidation of AB at a low applied potential and no reaction between MP and AB. When this combination was applied to an electrochemical immunosensor for parathyroid hormone (PTH) detection, a detection limit of 2 pg/mL was obtained. This detection limit is significantly lower than that obtained when a combination of phenol and AB was employed (300 pg/mL). It was also found that the developed immunosensor works well in PTH detection in clinical serum samples. This new phenolic substrate could therefore pave the way for Tyr to be more commonly used as a catalytic label in affinitybased biosensors.

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Tyrosinase Multilayer-Functionalised Carbon Nanotubes as Electrochemical Labels: Application To Immunoassay

Cited by 7 publications

References 45 publications

Electrochemical Immunoassay for Salmonella Typhimurium Based on an Immuno‐magnetic Redox Label

Electrochemical Immunoassay for Salmonella Typhimurium Based on an Immuno‐magnetic Redox Label

Overview on the Development of Electrochemical Immunosensors by the Signal Amplification of Enzyme- or Nanozyme-Based Catalysis Plus Redox Cycling

Phenolic Tyrosinase Substrate with a Formal Potential Lower than That of Phenol to Obtain a Sensitive Electrochemical Immunosensor

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