UBA domain protein SUF1 interacts with NatA‐complex subunit NAA15 to regulate thermotolerance in <i>Arabidopsis</i>

Song, Ze‐Ting; Chen, Xiao‐Jie; Luo, Lixia; Yu, Feifei; Han, Jiajia

doi:10.1111/jipb.13273

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…In C. elegans , NatA and NatC antagonistically regulate dauer larva formation under limited food, dense population and high temperature (Warnhoff et al ., 2014). Similarly, in plants, N‐terminal acetylation has been implicated in responses to drought, high salt, reductive stress, biotic immune challenges and heat stress (Hoshiyasu et al ., 2013; Linster et al ., 2015; Xu et al ., 2015; Armbruster et al ., 2020; Huber et al ., 2020; Gong et al ., 2022; Miklankova et al ., 2022; Song et al ., 2022). Despite the involvement of N‐terminal acetylation in stress responses, a knowledge gap persists regarding the connection between external stimuli and the N‐terminal acetylation system.…”

Section: Discussionmentioning

confidence: 99%

“…In plants, Nt‐acetylation has been implicated in responses to drought, high‐salt, reductive stress, and biotic immunity through phytohormones and sulfate assimilation (Hoshiyasu et al ., 2013; Linster et al ., 2015; Xu et al ., 2015; Armbruster et al ., 2020; Huber et al ., 2020; Gong et al ., 2022; Miklankova et al ., 2022). Several Nats subunits have been reported to influence basal thermotolerance in Arabidopsis (Song et al ., 2022). However, the precise role of Nt‐acetylation in plant temperature response remains poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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N‐terminal acetylation orchestrates glycolate‐mediated ROS homeostasis to promote rice thermoresponsive growth

Li,

Tang,

et al. 2024

New Phytologist

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Summary Climate warming poses a significant threat to global crop production and food security. However, our understanding of the molecular mechanisms governing thermoresponsive development in crops remains limited. Here we report that the auxiliary subunit of N‐terminal acetyltransferase A (NatA) in rice OsNAA15 is a prerequisite for rice thermoresponsive growth. OsNAA15 produces two isoforms OsNAA15.1 and OsNAA15.2, via temperature‐dependent alternative splicing. Among the two, OsNAA15.1 is more likely to form a stable and functional NatA complex with the potential catalytic subunit OsNAA10, leading to a thermoresponsive N‐terminal acetylome. Intriguingly, while OsNAA15.1 promotes plant growth under elevated temperatures, OsNAA15.2 exhibits an inhibitory effect. We identified two glycolate oxidases (GLO1/5) as major substrates from the thermoresponsive acetylome. These enzymes are involved in hydrogen peroxide (H2O2) biosynthesis via glycolate oxidation. N‐terminally acetylated GLO1/5 undergo their degradation through the ubiquitin‐proteasome system. This leads to reduced reactive oxygen species (ROS) production, thereby promoting plant growth, particularly under high ambient temperatures. Conclusively, our findings highlight the pivotal role of N‐terminal acetylation in orchestrating the glycolate‐mediated ROS homeostasis to facilitate thermoresponsive growth in rice.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

N‐terminal acetylation orchestrates glycolate‐mediated ROS homeostasis to promote rice thermoresponsive growth

Li,

Tang,

et al. 2024

New Phytologist

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“…Oxygen sensing in plants is mediated by a branch of the N-end rule pathway that controls the stability of constitutively expressed ERF-VII transcription factors, which are primary activators of core hypoxia response genes such as ADH, PCO, and SUS [42][43][44]. The N-end rule pathway is a part of the ubiquitin-proteasome degradation pathway and consists of two branches, the Ac/N-end rule, and Arg/N-end rule pathways [45,46]. ERF-VII proteins accumulate under hypoxic conditions to activate the expression of hypoxic response genes but are continuously degraded by the proteasome through the Arg/N-end rule pathway under normoxic conditions [42].…”

Section: Discussionmentioning

confidence: 99%

Proteasome Dysfunction Leads to Suppression of the Hypoxic Response Pathway in Arabidopsis

Xia

Tang

Chen

et al. 2022

IJMS

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Proteasome is a large proteolytic complex that consists of a 20S core particle (20SP) and 19S regulatory particle (19SP) in eukaryotes. The proteasome degrades most cellular proteins, thereby controlling many key processes, including gene expression and protein quality control. Proteasome dysfunction in plants leads to abnormal development and reduced adaptability to environmental stresses. Previous studies have shown that proteasome dysfunction upregulates the gene expression of proteasome subunits, which is known as the proteasome bounce-back response. However, the proteasome bounce-back response cannot explain the damaging effect of proteasome dysfunction on plant growth and stress adaptation. To address this question, we focused on downregulated genes caused by proteasome dysfunction. We first confirmed that the 20SP subunit PBE is an essential proteasome subunit in Arabidopsis and that PBE1 mutation impaired the function of the proteasome. Transcriptome analyses showed that hypoxia-responsive genes were greatly enriched in the downregulated genes in pbe1 mutants. Furthermore, we found that the pbe1 mutant is hypersensitive to waterlogging stress, a typical hypoxic condition, and hypoxia-related developments are impaired in the pbe1 mutant. Meanwhile, the 19SP subunit rpn1a mutant seedlings are also hypersensitive to waterlogging stress. In summary, our results suggested that proteasome dysfunction downregulated the hypoxia-responsive pathway and impaired plant growth and adaptability to hypoxia stress.

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HYPK controls stability and catalytic activity of the N-terminal acetyltransferase A in Arabidopsis thaliana

Gong,

Boyer,

Gierlich

et al. 2024

Cell Reports

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UBA domain protein SUF1 interacts with NatA‐complex subunit NAA15 to regulate thermotolerance in Arabidopsis

Cited by 6 publications

References 29 publications

N‐terminal acetylation orchestrates glycolate‐mediated ROS homeostasis to promote rice thermoresponsive growth

N‐terminal acetylation orchestrates glycolate‐mediated ROS homeostasis to promote rice thermoresponsive growth

Proteasome Dysfunction Leads to Suppression of the Hypoxic Response Pathway in Arabidopsis

HYPK controls stability and catalytic activity of the N-terminal acetyltransferase A in Arabidopsis thaliana

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