Über die quantitative Bindung von Ribonukleinsäure mit Gallozyaninchromalaun

Sandritter, W.; Diefenbach, H.; Krantz, F.

doi:10.1007/bf02159274

Cited by 40 publications

(1 citation statement)

References 6 publications

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“…The slides were removed after 24 and 48 h in culture. The preparations were fi xed in 96 o ethanol and stained by Feulgen's method in our modifi cation [5] for detection of DNA and with gallocyanin chrome alum [12] for detection of RNA, with methyl green pyronine [10] for detection of the nucleoli, with Mayer's hematoxylin and eosin [8] for cytomorphometry. DNA (λ=575 nm) and RNA (λ=634 nm) content was evaluated, the size of cells, nuclei, and nucleoli was estimated (at ×130) using an image analyzer designed on the basis of an SMP-05 scanning photometer microscope (Opton) using UPIAM-2000 software (Yu.…”

Section: Methodsmentioning

confidence: 99%

Comparative Multiparametric Analysis of HeLa and RD Cell Culture Reactions to Solcoseryl

et al. 2009

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Reactions of continuous HeLa and RD cell cultures and their nuclear and nucleolar apparatus to addition of solcoseryl into the medium were studied. The monolayer density, proliferation activity, percentage of dead cells, RNA and DNA content in the nuclei and nucleoli, number of nucleoli in the nuclei, cell distribution in the population by the number of nucleoli in the nuclei, volume and complete surface area of the nuclei and nucleoli, and the nucleolar/nuclear ratio were evaluated. The cultures differently reacted to solcoseryl in the medium at the population and cellular levels of their organization. By the results of multiparametric analysis of the reactions of cells and their nuclear and nucleolar apparatus, solcoseryl can be referred to bioactive substances with characteristics of a factor regulating cell population growth.

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Section: Methodsmentioning

confidence: 99%