Über die Wirkung von Komplexbildnern und Metallkationen auf die enzymatische Oxydation von Ketoglutarsäure

Brenner‐Holzach, O.; Raaflaub, J

doi:10.1002/hlca.19560390330

Cited by 8 publications

(10 citation statements)

References 6 publications

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“…5). This correlation is closer than the correlation between spontaneous swelling and intramitochondrial ATP (36), which falls to one-fifth its normal value before spontaneous swelling ensues. There is also no correlation with oxidative phosphorylation.…”

Section: Existence Of Different Types and Stages Of Milochondrial Swementioning

confidence: 52%

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The Swelling of Rat Liver Mitochondria by Thyroxine and its Reversal

Lehninger¹,

Ray²,

Schneider³

1959

The Journal of Cell Biology

143

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The in vitro swelling action of L-thyroxine on rat liver mitochondria as examined photometrically represents an acceleration of a process which the mitochondria are already inherently capable of undergoing spontaneously, as indicated by the identical kinetic characteristics and the extent of thyroxine-induced and spontaneous swelling, the nearly identical pH dependence, and the fact that sucrose has a specific inhibitory action on both types of swelling. However, thyroxine does not appear to be a "catalyst" or coenzyme since it does not decrease the temperature coefficient of spontaneous swelling. The temperature coefficient is very high, approximatNy 6.0 near 20 °.Aging of mitochondria at 0 ° causes loss of thyroxine sensitivity which correlates closely with the loss of bound DPN from the mitochondria, but not with loss of activity of the respiratory chain or with the efficiency of oxidative phosphorylation. Tests with various respiratory chain inhibitors showed that the oxidation state of bound DPN may be a major determinant of thyroxine sensitivity; the oxidation state of the other respiratory carriers does not appear to influence sensitivity to thyroxine. These facts and other considerations suggest that a bound form of mitochondrial DPN is the "target" of the action of thyroxine.The thyroxine-induced swelling is not reversed by increasing the osmolar concentration of external sucrose, but can be "passively" or osmotically reversed by adding the high-particle weight solute polyvinylpyrrolidone. The mitochondrial membrane becomes more permeable to sucrose during the swelling reaction. On the other hand, thyroxine-induced swelling can be "actively" reversed by ATP in a medium of 0.15 M KC1 or NaC1 but not in a 0.30 ~ sucrose medium. The action of ATP is specific; ADP, Mn +*, and ethylenediaminetetraacetate are not active. It is concluded that sucrose is an inhibitor of the enzymatic relationship between oxidative phosphorylation and the contractility and permeability properties of the mitochondrial membrane.Occurrence of different types of mitochondrial swelling, the intracellular factors affecting the swelling and shrinking of mitochondria, as well as the physiological significance of thyroxine-induced swelling are discussed.

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Section: Existence Of Different Types and Stages Of Milochondrial Swementioning

confidence: 52%

“…However, it is also known that spontaneous swelling is accompanied by destruction of intramitochondrial ATP (17,36). action of thyroxine.…”

Section: Effect Of Aging Of Mitochondria--sensitivitymentioning

confidence: 99%

The Swelling of Rat Liver Mitochondria by Thyroxine and its Reversal

Lehninger¹,

Ray²,

Schneider³

1959

The Journal of Cell Biology

143

View full text Add to dashboard Cite

show abstract

“…5 above). The relation for substrates other than succinate is explained by the diffusion of coenzymes into the medium during swelling (Brenner-Holzach & Raaflaub, 1954;Hunter & Ford, 1955). Where oxidative phosphorylation is necessary to maintain respiring mitochondria in a contracted state, an interdependency exists: ATP maintains the contracted state and this state is essential for the preservation ofcoenzymes necessary for the generation of ATP.…”

Section: Discussionmentioning

confidence: 99%

Movements of water and ions in mitochondria

Price

Fonnesu

Davies

1956

Biochemical Journal

141

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“…The maintenance of a low water content and a higher potassium concentration within mitochondria is a process which requires metabolism or 'high-energy' phosphates (Macfarlane & Spencer, 1953;Price & Davies, 1954;Brenner-Holzach & IC ( Table 12. Effect of Co I on the oxidation of P-hydroxybutyrate and L-matate by rat-liver mitochondria in the presence of triethyltin 8ulphate…”

Section: Biochemistry Of Ormentioning

confidence: 99%