Überexpression von β1-Integrin in oralen Keratinozyten korreliert mit deren hohen Proliferationskapazität

Abstract

Our results show that it is possible to enrich a fraction of highly proliferative oral keratinocytes by means of their high affinity to collagen type IV.

In vitro study on proliferation kinetics of oral mucosal keratinocytes

“…Cell populations with a high affinity to culture dishes coated with collagen type IV consisted predominantly of small highly proliferative cells with high levels of b1-integrin. 3 Laminin has been described as a component of the basal membrane, such as collagen type IV, nidogen, and proteoglycan. These proteins are being produced by keratinocytes and fibroblasts and form a macromolecular network, which serves the epithelial cell and acts as a barrier and filter.…”

“…10 Their high proliferative potential makes them ideal to be used in TE in order to achieve a large population of cells in as short a time as possible. 3 Three different kinds of epidermal stem cells can be differentiated using expression levels of b1-subunits of *These authors contributed equally to the paper.…”

“…2 Moreover, the unpredictable survival of the graft, contraction of the graft, growth of hair on the graft, a loss of function, donor site morbidity, and difficulties in prosthodontic treatment are unsolved problems in soft tissue transplantation of oral mucosa. 3 The replacement of lost tissue with autogenous cells and the use of scaffolds are adapted to the concept of tissue engineering (TE). In TE, autogenous cells are isolated, cultivated, and augmented using cell culture until enough cells for the colonization of scaffold structures are available.…”

Advanced therapies of skin injuries

“…Novel potential skin substitute biomaterials and scaffolds include human hair keratin-collagen sponge, hyaluronan coupled with fibronectin functional domains, poly(lactic-co-glycolic acid)/chitosan hybrid nanofibrous membrane, biodegradable polyurethane microfibres, polycaprolactone (PCL) collagen nanofibrous membranes, silk fibroin and alginates, polyvinyl alcohol/ chitosan/fibroin blended sponges, Tegaderm-nanofiber constructs, bacterial cellulose, ICX-SKN skin graft replacements, porcine collagen paste, bovine collagen cross-linked with microbial transglutaminase, collagenglycosaminoglycan-chitosan dermal matrix seeded with fibroblasts, composite nano-titanium oxide-chitosan artificial skin, keratinocytes and fibroblasts grown on Collatamp, deacetylated chitin or plant cellulose transfer membranes, and many others [17,56,57]. New materials could also be found and evaluated by novel computational methods [58].…”

Epithelial–Mesenchymal Interactions as a Working Concept for Oral Mucosa Regeneration

“…Oral epithelial cells appear to proliferate at a higher rate than skin keratinocytes. 69 Cell morphology and keratin expression of cultured oral epithelial cells seem to depend on the site of origin in the oral cavity. During the past 20 years, epidermal sheets have been applied for the reconstruction of oral mucosal defects.…”