Ubiquitin genes in rainbow trout ( )

Okubo, Kazue; Yamano, Keisuke; Qin, Qi; Aoyagi, Kazuhiko; Ototake, Mitsuru; Nakanishi, Teruyuki; Fukuda, Hideo; Dijkstra, Johannes M.

doi:10.1006/fsim.2001.0375

Cited by 8 publications

(2 citation statements)

References 43 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polyubiquitinated proteins are then targeted to the 26S proteasome for degradation [8,9,13,24]. Many viruses are known to hijack the host ubiquitination pathway where the host proteins are redirected for degradation in the 26S proteasome [23,15]. This specific process often involves an E3 ubiquitin ligase that is directly encoded by either the virus or the host genome [2].…”

Section: Discussionmentioning

confidence: 99%

Expression Profile of Penaeus monodon Ubiquitin Conjugating Enzyme (PmUbc) at Protein Level in White spot syndrome virus Challenged Shrimp

et al. 2013

View full text Add to dashboard Cite

White spot syndrome virus (WSSV) is one of the major pathogens in shrimp aquaculture. Four proteins of WSSV are predicted to encode a RING H2 domain, which in presence of ubiquitin conjugating enzyme (E2) in shrimps can function as viral E3 ligase and modulate the host ubiquitin proteasome pathway. Modulation of host ubiquitin proteasome pathway by viral proteins is implicated in viral pathogenesis. In the present study, expression profile of Penaeus monodon Ubiquitin conjugating enzyme (PmUbc) was studied at protein level in WSSV challenged shrimp. A time point analysis of the expression of PmUbc was carried out at 0, 3, 6, 12, 24, 48 and 72 h post WSSV challenge in P. monodon. Recombinant PmUbc (rPmUbc) was produced in prokaryotic expression vector, BL21 (DE3) pLys S. The PmUbc expression pattern was studied by ELISA with rPmUbc antibodies raised in rabbit. A significant increase in PmUbc expression at 24 h post infection (hpi) was observed followed by a decline till 72 hpi. Since the up-regulation and a tremendous decline of PmUbc protein expression was observed at 24 and in 72 hpi respectively in ELISA, it can be speculated that these proteins might interact with host ubiquitination pathway for viral pathogenesis. Many findings have shown that viral infection can up-regulate expression of ubiquitin and that the ubiquitin system plays a key role in the course of viral infection. The present study reveals the expression patterns of PmUbc at protein level in WSSV infected P. monodon. However, further studies are to be carried out to unfold the molecular mechanism of interaction between host and virus to devise efficient control strategies for this major culprit in shrimp culture industry.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression Profile of Penaeus monodon Ubiquitin Conjugating Enzyme (PmUbc) at Protein Level in White spot syndrome virus Challenged Shrimp

et al. 2013

View full text Add to dashboard Cite

show abstract

“…2), the deduced amino acid sequence was found to be fully conserved and 100% similar to that of the polyubiquitin sequence of various species. Similarly, Okubo et al (2002) reported that in rainbow trout, although the DNA sequence is not conserved, the encoded amino acid sequence is fully conserved. The complete conservation of the amino acid sequence of ubiquitin, which was observed in the functional genes that have been sequenced, is in accordance with the fact that ubiquitin is completely conserved through evolution.…”

Section: Setmentioning

confidence: 94%

Characterization of the Nucleotide Sequence of a Polyubiquitin Gene (PUBC1) from Arabian Camel, Camelus dromedarius

Al‐Khedhairy¹

2004

BMB Reports

View full text Add to dashboard Cite

Molecular amplification and sequencing of genomic DNA that encodes camel polyubiquitin (PUBC1) was performed by a polymerase chain reaction (PCR) using various sets of primers. The amplification generated a number of DNA fragments, which were sequenced and compared with the polyubiquitin coding sequences of various species. One DNA fragment that conformed to 325 bp was found to be 95 and 88% homologous to the sequences of human polyubiquitin B and C, respectively. The DNA translated into 108 amino acids that corresponded to two fused units of ubiquitin with no intervening sequence, which indicates that it is a polyubiquitin and contains at least two units of ubiquitin. Although, variations were found in the nucleotide sequence when compared to those of other species, the amino acid sequence was 100% homologous to the polyubiquitin sequences of humans, mice, and rats. This is the first report of the polyubiquitin DNA coding sequence and its corresponding amino acid sequence from camels, amplified using direct genomic DNA preparations.

show abstract

The promoter of the classical MHC class I locus in rainbow trout (Oncorhynchus mykiss)

Dijkstra

Yoshiura

Kiryu

et al. 2003

Fish & Shellfish Immunology

Self Cite

View full text Add to dashboard Cite

Ubiquitin genes in rainbow trout ( )

Cited by 8 publications

References 43 publications

Expression Profile of Penaeus monodon Ubiquitin Conjugating Enzyme (PmUbc) at Protein Level in White spot syndrome virus Challenged Shrimp

Expression Profile of Penaeus monodon Ubiquitin Conjugating Enzyme (PmUbc) at Protein Level in White spot syndrome virus Challenged Shrimp

Characterization of the Nucleotide Sequence of a Polyubiquitin Gene (PUBC1) from Arabian Camel, Camelus dromedarius

The promoter of the classical MHC class I locus in rainbow trout (Oncorhynchus mykiss)

Contact Info

Product

Resources

About