UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TALAS (Colococasia Esculenta L.) TERHADAP BAKTERI Escherichia coli

Abstract

Salah satu tanaman yang sering dijadikan obat adalah daun Talas (Colocasia esculanta L.). Daun talas memiliki kandungan senyawa flavonoid, alkaloid, saponin dan tannin yang memiliki kemampuan untuk menghambat pertumbuhan bakteri. Eschericia coli adalah salah satu jenis bakteri yang dapat menyebabkan infeksi pada pencernaan. Salah satu tujuan penelitian ini untuk mengetahui aktivitas antibakteri daun talas dengan konsentrasi yang paling efektif dalam menghambat bakteri Eschericia coli. Penelitian ini adalah penelitian eksperimental murni. Daun talas aktivitas antibakteri dilakukan dengan metode difusi cakram. Penelitian dilakukan dengan 3 variasi konsentrasi yang berbeda. Hasil penelitian menunjukkan bahwa ada aktivitas antibakteri pada setiap konsentrasi. Kesimpulan pemberian aktivitas daun talas dengan konsentrasi 15%, 25%, dan 35% memberikan daya hambat pertumbuhan bakteri paling efektif dengan kosentrasi 25%, 35% yang termasuk dalam kategori kuat. Saran diharapkan kepada peneliti selanjutnya dapat melakukan uji terhadap bakteri lain dan membuat formulasi dari ekstrak daun talas.

Antibacterial Activity Test of the Combination of Methanol Extract of Arumanis Mango Leaves (Mangifera indica L) and Taro Leaves (Colocasia esculanta L) on Escherichia coli Bacteria Causes of Diarrhea Diseases

“…The results of the phytochemical screening test on arumanis mango leaf extract are the same as the results of research conducted by [16], which states that arumanis mango leaves contain secondary metabolite compounds in the form of flavonoids, tannins, and saponins. The tube test results on taro leaf extract are similar to the research conducted by [8] in that taro leaves contain secondary metabolite compounds in the form of alkaloids, saponins, flavonoids, and tannins. Research results [16] also stated that arumanis mango leaves contain secondary metabolite compounds in flavonoids, tannins, and saponins.…”

“…The results showed that the purified extract of arumanis mango leaves ranging from a concentration of 6.25% to 100% indicated that the results of the diameter of the inhibition zone produced the higher the concentration, the greater the diameter of the inhibition zone made successively, namely 15.69 ± 0.76 mm to 24.15 ± 0.05 mm. According to research [8], Taro leaf extract at a concentration of 35% has an average inhibition zone diameter of 14.24 mm, which is included in the strong category. Arumanis mango leaves and taro leaves contain secondary metabolite compounds that have the potential to be antibacterials, so they can be combined to determine how much antibacterial activity they have.…”

“…The research [23] stated that the ethanol extract of arumanis mango leaves has potential antibacterial activity by producing an inhibition zone of 14.8 mm within 1x24 hours, and at 5x24 hours of observation, the diameter of the inhibition zone increased by 16.2 mm, which is included in the strong category. The research [8] stated that the ethanol extract of taro leaves has an average diameter of the inhibition zone at a concentration of 15% of 9.35 mm, a concentration of 25% of 11.45 mm, and at a concentration of 35% of 14.24 mm which is included in the strong category. This shows that flavonoid and tannin compounds have antibacterial activity, where flavonoid compounds act as antibacterials by forming complex compounds in extracellular proteins that cause bacterial cell proteins to denature to damage the cell membrane [24].…”

“…According to research by [7], arumanis mango leaves contain flavonoids, alkaloids, steroids, polyphenols, tannins, and saponins. Research [8] shows that taro leaves contain alkaloids, saponins, flavonoids, and tannins. Secondary metabolites that function as antibacterials are flavonoids and tannins.…”

Antibacterial effect of corarian seed (Coriandrum sativum L.) extract on Streptococcus mutans

“…Zone differences occur because of the different levels of the active substance from each concentration which is influenced by the dilution series. The more active substances dissolved, the larger the diameter of the inhibition zone formed, this is also due to the presence of saponin active substances, According to (Rahmawati et al, 2014), several factors that can affect the work of antimicrobial materials are: concentration or intensity of antimicrobial substances, the higher the concentration of antimicrobial substances, the higher the inhibitory or killing power (to a certain extent), the number, type, age and condition of microorganisms, pH or acidity because microorganisms present in acidic materials can be eradicated at lower temperatures in a shorter time than the same microorganisms in an alkaline environment, an increase in temperature can increase the effectiveness of an antimicrobial agent. Every 10 o C increase can cause a doubling of the mortality rate, besides that microorganisms that are long enough in anti-microbial materials can be stunted or can die.…”