UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL LIDAH  BUAYA (Aloe vera) TERHADAP BAKTERI Staphylococcus aureus

Rahardjo, M. Budi; Koendhori, Eko Budi; Setiawati, Yuani

doi:10.24815/jks.v17i2.8975

Cited by 16 publications

(17 citation statements)

References 2 publications

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“…Lidah buaya diketahui memiliki berbagai zat aktif yang diketahui dapat menghambat pertumbuhan bakteri (Yebpella et al, 2011;Sulistyani et al, 2016;Rahardjo et al, 2017). Berbagai mikroba dapat dihambat pertumbuhannya oleh lidah buaya seperti Staphylococus aureus, Enterobacter faecalis, Streptococcus mutans, Candida albicans, Mycobacterium smegmatis, dan Klebsiella pneumonia serta E. coli dari manusia (Aswarita, 2013;Rahardjo et al, 2017;Agrawal et al, 2018). Efektivitas lidah buaya dalam menghambat pertumbuhan E. coli sumber air minum broiler belum diketahui.…”

Section: Kata Kunciunclassified

“…Fenol merupakan zat yang dapat menjaga komponen aktif yang terkandung pada ekstrak tanaman dari oksidasi (Mulyanita, 2019). Kadar zat aktif pada infusa aquades menurun dikarenakan oksidasi akibat lama terpapar oleh udara (Rahardjo et al, 2017). Perlakuan infusa aquades pada penelitian ini mengalami oksidasi pada saat pemrosesan karena memakan waktu yang cukup lama sehingga menghilangkan zat aktif yang dapat menghambat pertumbuhan bakteri.…”

Section: Penelitian Ini Dilaksanakan Pada Bulanunclassified

“…Senyawa lain yang memiliki aktivitas antibakteri pada lidah buaya adalah antraquinon. Antrakuinon menginaktivasi protein bakteri melalui ikatan antrakuinon (Rahardjo et al, 2017). Zat-zat ini beperan dalam aktivitas inhibisi ekstrak ethanol lidah buaya pada penelitian ini sehingga membentuk zona hambat pada media MHA.…”

Section: Penelitian Ini Dilaksanakan Pada Bulanunclassified

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Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya

Nuraini¹,

Andityas²,

Paramarta³

et al. 2020

J. Ilmu Peternak. dan Vet. Trop. (Journal Trop. Anim. Vet. Sci.

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Abstract Colibacillosis is one of the most problematic issues in the boiler industry. However, the antibiotic overuse has induced Escherichia coli resistance so that other alternative to reduce colibacillosis is needed. One of the alternatives is using aloe vera (Aloe barbadensis Miller), which has been widely used as an antibacterial agent. This study aims to isolate and identify E. coli from the broiler drinking water source and test the aloe vera antibacterial activity against it. Escherichia coli were isolated from well in three broiler farms in Moyudan District, Sleman, Yogyakarta that previously had colibacillosis. Escherichia coli were isolated using eosin methylene blue (EMB) agar and the metallic sheen colony was tested to confirm the biochemist reaction. The pure isolate of E. coli was used in the aloe vera inhibition test using Muller Hinton agar (MHA) by a Well Diffusion method. Aloe vera was processed using aquades and ethanol 70%. The aquades infusion was diluted into 12.5%, 25%, 50%, 75%, and 100% and the extract ethanol 70% was diluted into 10%, 12.5, %, 25%, 40%, and 50%. The bacterial identification showed that one of three samples contained E. coli which was then used for inhibition test. The result showed no inhibition zone in the aquades infusion while ethanol extract showed an inhibition zone in concentration 25%, 40%, and 50% of aloe vera extract with a diameter 19.5 mm, 24 mm, and 25 mm. It can be concluded that aloe vera ethanol extract has inhibitory activity against E. coli in poultry drinking water with a minimum concentration of 25%. Keywords: Aloe vera; Broiler drinking water; Escherichia coli; Inhibitory activity Abstrak Colibacilosis masih menjadi permasalahan dalam industri broiler. Penggunaan antibiotik berlebihan telah menyebabkan resistensi sehingga perlu alternatif lain. Salah satu alternatif adalah menggunakan bahan alami seperti adalah lidah buaya (Aloe barbadensis Miller) yang memilliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi E. coli dari sumber air minum di kandang broiler serta menguji aktivitas inhibisi lidah buaya terhadap bakteri tersebut. Air yang digunakan sebagai sumber E. coli berasal dari sumur di tiga peternakan broiler di Kecamatan Moyudan, Sleman, Yogyakarta yang memiliki riwayat infeksi colibacilosis. Bakteri E. coli diisolasi menggunakan media eosin methylene blue (EMB) dan diuji sifat biokimia untuk mengkonfirmasi sifat bakteri E. coli. Isolat murni E. coli digunakan pada uji daya hambat bakteri dengan metode difusi sumuran menggunakan media Muller Hinton Agar (MHA). Lidah buaya diproses menggunakan aquades dan ethanol 70%. Infusa aquades diencerkan menjadi konsentrasi 12,5%, 25%, 50%, 75%, dan 100% dan ekstrak ethanol 70% diencerkan menjadi 10%, 12., %, 25%, 40%, dan 50%. Hasil isolasi menunjukan bahwa satu sumber air dari sumur di Desa Kolowenang mengandung E. coli yang kemudian digunakan pada pengujian daya hambat. Hasil pengujian menunjukan tidak ada daya hambat yang terbentuk pada infusa aquades sedangkan ekstrak etanol lidah buaya 25%, 40%, dan 50% menunjukan adanya zona hambat sebesar 19,5 mm, 24 mm, dan 25 mm berturut-turut. Ekstrak etanol lidah buaya pada penelitian ini memiliki kemampuan menghambat pertumbuhan E. coli yang bersumber dari air minum broiler dengan konsentrasi terendah 25%. Kata kunci: Air minum broiler; Escherichia coli; Lidah buaya; Daya hambat bakteri

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Section: Kata Kunciunclassified

Section: Penelitian Ini Dilaksanakan Pada Bulanunclassified

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Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya

Nuraini¹,

Andityas²,

Paramarta³

et al. 2020

J. Ilmu Peternak. dan Vet. Trop. (Journal Trop. Anim. Vet. Sci.

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“…The decreaced of Aloe vera effectiveness as antimicrobial influenced by exposure to air of Aloe vera gel resulting in rapidly oxidized, decomposed and lose its biological activity. The antibacterial activity not only comes from Aloe vera gel, but also other active compounds in the plant, because the polysaccharide in the gel is unstable to heat, acid, and enzyme (Rahardjo et al, 2017).…”

Section: Barrier Zone Of Edible Filmmentioning

confidence: 99%

Study of Physical Characteristic, Water Vapor Transmission Rate and Inhibition Zones of Edible Films from Aloe vera (Aloe barbadensis) Incorporated with Yellow Sweet Potato Starch and Glycerol

Rahmiatiningrum

Sukardi

Warkoyo

2019

Food Technol. Halal Sci.

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Glucomannan was the main polysaccharide of Aloe vera gel. It was dissolved in water, formed a gel, and transparent as a film. Aloe vera gel was reported antimicrobial and antioxidant activity such as saponin and anthraquinone that was potential for the increased value of an edible film. However, Aloe vera gel form weak film caused glucomannan to have high water absorption. In this research, Aloe vera gel was used as a basis for the polymer film. Yellow sweet potato starch added for the strength matrix component used amylose. This starch expected to give colors from carotenoids. Glycerol also added for the flexibility of an edible film. Randomized Complete Block Design Factorial (RCBD) was applied. The first factor was concentration of yellow sweet potato starch (1%, 2%, 3%) and the second one was glycerol (0,1%, 0,25%, and 0,5%). The parameters tested were color, thickness, transparency, tensile strength, elongation, solubility, water vapor transmission rate, and inhibition zones against E. coli, and S. aureus, fungi A. niger and C. Albicans. The results showed that the addition of yellow sweet potato starch and glycerol with different concentration had a significant effect on color, thickness, transparency, tensile strength, elongation, and solubility. However, an edible film on this research has not to show bacteria and fungi inhibition zone of edible film. P2G1 is the best treatment (yellow sweet potato starch 2% and glycerol 0,1%) produced an edible film with a thickness of 0.12mm, elongation 50.85%, tensile strength 0.55 MPa, solubility 41.03%, transparency 2.13%, vapor transmission rate 3,40 g/m2/24hours, L, a+, b+ score in sequence 41.87, 0.2, and 4.1.

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“…Hal ini dikarenakan adanya beberapa kasus resistensi pada antibiotik. Selain karena resistensi, pengguna antibiotik memerlukan biaya yang belum tentu dapat dicapai oleh masyarakat umum (Rahardjo, 2017).…”

Section: Pendahuluanunclassified

UJI DAYA HAMBAT ORGANISME LAUT SPONS Amphimedon sp., TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus, Escherichia coli, DAN Jamur Candida albicans

Latif¹,

Wewengkang²,

Rotinsulu³

2019

PHA

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Sponges are marine animals that can produce bioactives that are useful as anti-virus, anti-fungal, antibiotic, anti-cancer, anti-inflammatory, and antioxidants. Amphimedon sp., sponge itself is found in the waters of Lembeh islands, the City of Bitung. This study was to determine the inhibitory activity against the microorganisms growth of Amphimedon sp., sponge, against microbes of Staphylococcus aureus, Escherichia coli, and Candida albicans. Based on the results of the study, the extracts and fractions of Amphimedon sp., sponge samples did not have microbial inhibitory activity against the test microbes of Staphylococcus aureus, Escherichia coli, and Candida albicans Keywords : Amphimedon sp., Antimicrobial, Staphylococcus aureus, Escherichia coli, Candida albicans ABSTRAK Spons merupakan hewan laut yang dapat menghasilkan bioaktif yang bermanfaat sebagai anti virus, anti jamur, antibiotik, anti kanker, anti inflamasi, dan antioksidan. Spons Amphimedon sp sendiri ditemukan didaerah perairan pulau Lembeh kota bitung. Penelitian ini yaitu untuk mengetahui aktivitas daya hambat pertumbuhan mikroorganisme dari spons Amphimedon sp., terhadap mikroba Staphylococcus aureus, Escherichia coli, dan Candida albicans. Berdasarkan hasil penelitian menunjukkan ekstrak dan fraksi sampel spons Amphimedon sp., tidak memiliki aktivitas daya hambat terhadap mikroba uji Staphylococcus aureus, Escherichia coli, dan Candida albicans Kata kunci : Amphimedon sp., Antimikroba, Staphylococcus aureus, Escherichia coli, Candida albicans

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL LIDAH BUAYA (Aloe vera) TERHADAP BAKTERI Staphylococcus aureus

Cited by 16 publications

References 2 publications

Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya

Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya

Study of Physical Characteristic, Water Vapor Transmission Rate and Inhibition Zones of Edible Films from Aloe vera (Aloe barbadensis) Incorporated with Yellow Sweet Potato Starch and Glycerol

UJI DAYA HAMBAT ORGANISME LAUT SPONS Amphimedon sp., TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus, Escherichia coli, DAN Jamur Candida albicans

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