Uji Aktivitas Antioksidan Ekstrak Metanol Kulit Pisang Raja (Musa Paradisiaca sapientum) Dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrazil)

Jami’ah, Sitti Raudhotul; Ifaya, Mus; Pusmarani, Jastria; Nurhikma, Eny

doi:10.35311/jmpi.v4i1.22

Cited by 20 publications

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“…The leaf and fruit extracts were tested for antioxidant activity using the DPPH method. 13 The stock solution was made by adding various concentrations of fruit and leaf extracts and dissolving in 5 mL absolute methanol. A control solution containing 3 mL of methanol and 1 mL of DPPH 100 ppm solution was also prepared.…”

Section: Methodsmentioning

confidence: 99%

Anticancer Activity of Methanol Extract of Ficus carica Leaves and Fruits Against Proliferation, Apoptosis, and Necrosis in Huh7it Cells

et al. 2019

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The polyphenol plant extracts have previously been demonstrated to act as chemopreventive and anticancer agents. Ficus carica is a rich source of polyphenols, yet its antioxidant and anticancer activities remain poorly characterized. This study aimed to determine the anticancer activity of F carica leaf and fruit extracts by investigating their impact on proliferation, apoptosis, and Huh7it cell necrosis. Leaves and fruits were extracted using methanol, and the phytochemical contents were analyzed using Fourier-transform infrared spectroscopy. The antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl method. Anticancer activities were examined through MTT (3-(4,5-dimethylthiazol-2yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay on Huh7it liver cancer cells. The apoptosis and necrosis conditions were examined using Annexin biomarkers V-PI and later analyzed in flow cytometry. F carica leaves and fruit examined were found to have strong antioxidant activities with IC 50 values of 7.9875 µg/mL and 13.402 µg/mL, respectively. MTT assay results indicated F carica leaves and fruit had IC 50 values >653 μg/mL and >2000 μg/mL, respectively. The flow cytometry analysis indicated a higher percentage of Huh7it apoptosis and necrosis in leaf extracts compared with fruit extracts. The difference in anticancer activity was attributed to differing compounds present in each extract.

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Section: Methodsmentioning

confidence: 99%

Anticancer Activity of Methanol Extract of Ficus carica Leaves and Fruits Against Proliferation, Apoptosis, and Necrosis in Huh7it Cells

et al. 2019

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“…kromatografi senyawa aktif antioksidan [24] Setiap fraksi daun Sembung ditotolkan pada plat KLT kemudian dielusi menggunakan eluen n-heksan : etil asetat (1:2) dan diamati pada sinar UV 254 nm dan 366 nm. Perubahan warna noda menjadi kuning pucat setelah disemprot larutan DPPH menunjukkan adanya aktivitas antioksidan [22] .…”

Section: Profilunclassified

“…Sedangkan pengujian aktivitas antioksidan menggunakan metode serapan radikal bebas DPPH. Pemilihan metoda ini dikarenakan tidak membutuhkan banyak reagen, cepat dan sederhana [22] .…”

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Uji Aktivitas Antioksidan dan Fenolik Total Fraksi Etil Asetat Daun Sembung (Blumea balsamifera (L.) DC)

et al. 2021

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The aim of this study is to investigate antioxidant activity and total phenolic content of ethyl acetate fraction of Sembung leaves. Sembung (Blumea balsamifera L.) is a plant that has the potential as an antioxidant because it contains secondary metabolites of alkaloids, steroids, flavonoids, saponins, and phenolic compounds. In this study, antioxidant activity was tested by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) for free radical scavenging activity assay. Evaluation of total phenolic content was tested by using the Folin-Ciocalteau method. The results showed that the ethyl acetate fraction of Sembung leaves possess showed medium antioxidant activity with IC50 valueof 221.821 compare with reference standard ascorbic acid with IC50 value 68.25 ppm. The ethyl acetate fraction of Sembung leaves has a total phenolic content of 161.101 mg GAE/g. This study provided that Blumea balsamifera leaves possess antioxidant.

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“…Aktivitas peredaman radikal DPPH ditunjukkan dengan persen peredaman. Harga persentase peredaman radikal bebas DPPH dapat dihitung dengan persamaan 7 di bawah ini [28].…”

Section: Metode 11-diphenyl-2-picrylhydrazyl (Dpph)unclassified

Review Artikel: Potensi Bunga Tanaman Sukun (Artocarpus Altilis [Park. I] Fosberg) Sebagai Bahan Antioksidan Alami

Kurniawati

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2021

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Abstrak. Review artikel ini ditujukan untuk membahas tentang potensi bunga tanaman sukun sebagai antioksidan. Sukun (Artocarpus altilis) merupakan salah satu tanaman yang menjadi kekayaan alam hayati Indonesia. Tanaman tersebut telah dimanfaatkan masyarakat sebagai bahan pangan, bahan peralatan rumah tangga, serta obat tradisional untuk mengobati penyakit rematik, diabetes, penyakit jantung, sariawan, gangguan hati, asam urat, radang sendi, gangguan ginjal, panu, hipertensi, dan menurunkan kolesterol. Bunga sukun mengandung senyawa metabolit sekunder golongan flavonoid, tanin dan saponin. Senyawa flavonoid dan tanin merupakan senyawa fenolik yang memiliki aktivitas antioksidan, sehingga mampu menghambat proses oksidasi yang disebabkan oleh radikal bebas. Aktivitas antioksidan dapat ditentukan dengan metode antara lain DPPH, ABTS, FRAP, CUPRAC, dan ORAC.Kata kunci : Artocarpus altilis, bunga sukun, antioksidanAbstract. This review article is intended to discuss the potential of breadfruit plant flowers as antioxidants. Breadfruit (Artocarpus altilis) is one of the plants that is part of Indonesia's natural resources. These plants have been used by the community as food, household utensils, and traditional medicines to treat rheumatism, diabetes, heart disease, mouth sores, liver disorders, gout, arthritis, kidney problems, tinea versicolor, hypertension, and lowering cholesterol. Breadfruit flowers contain secondary metabolite compounds, flavonoids, tannins and saponins. Flavonoids and tannins are phenolic compounds that have antioxidant activity, so they can inhibit the oxidation process caused by free radicals. Antioxidant activity can be determined by methods including DPPH, ABTS, FRAP, CUPRAC, and ORAC.Key words: Artocarpus altilis, breadfuit flowers, antioxidant

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Uji Aktivitas Antioksidan Ekstrak Metanol Kulit Pisang Raja (Musa Paradisiaca sapientum) Dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrazil)

Cited by 20 publications

References 0 publications

Anticancer Activity of Methanol Extract of Ficus carica Leaves and Fruits Against Proliferation, Apoptosis, and Necrosis in Huh7it Cells

Anticancer Activity of Methanol Extract of Ficus carica Leaves and Fruits Against Proliferation, Apoptosis, and Necrosis in Huh7it Cells

Uji Aktivitas Antioksidan dan Fenolik Total Fraksi Etil Asetat Daun Sembung (Blumea balsamifera (L.) DC)

Review Artikel: Potensi Bunga Tanaman Sukun (Artocarpus Altilis [Park. I] Fosberg) Sebagai Bahan Antioksidan Alami

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