UJI FITOKIMIA EKSTRAK ETANOL DAUN KEMANGI (Ocimum basilicum L)

Abstract

Basil leaves (Ocimum basilicum L) was one of the plants that thrive in Indonesia. This plant could be used as an antipyretic, antifungal, analgesic, antiseptic, antibacterial, hepatoprotector, immunomodulator, antireppelent and antiexpectorant. The chemical content found in this plant has a role in providing pharmacological activities so that this research was conducted to develop the use of basil leaves as natural medicine. Phytochemical screening aims to provide an overview of the classes of compounds contained in basil leaves which include flavonoids, alkaloids, saponins and tannins. The samples were determined and identified in the Integrated Laboratory of Sunan Ampel UIN Surabaya. The extract was made by extracting basil leaf simplicia with ethanol solvent by maceration method and doing phytochemical tests by adding solvents by observing changes in colour and shape of the solution. Phytochemical test results show that the positive basil leaves contain flavonoid compounds (marked by the appearance of black), alkaloids (characterized by the presence of a brownish yellow colour and precipitate), saponins (marked by the stable foam), and tannins (marked by the green colour black).

The Hepatoprotective Effects of Basil Leaf (Ocimum sanctum L.) Extract on Paracetamol Induced Liver Damage in Male Rat

“…The addition of Mg and HCl in this test will reduce the flavonoid compounds present, causing a red, orange or yellow reaction. 13…”

“…10 ml of filtar was extracted with 10 ml of 1: 10 HCl solution then dropped on filter paper and dripped with dragendroff reagent if it was red or orange and the addition of mayer reagent to the filtrate would produce a white precipitate indicating the presence of alkaloid compounds. 13 3. Saponin test Assignment Test…”

“…10 ml of the solution obtained from the identification of flavonoids was put into a test tube and shaken quickly. On examination of the saponin content, a positive test was shown by the formation of foam and was stable for 10 minutes13 4. Test the tannins 10 ml of the solution obtained from the identification of flavonoids was inserted into the test tube.…”

“…Then filter and add 1 ml of 10% FeCl3 until the color changes. The examination of the positive test for tannin content is indicated by the appearance of a blackish blue or blackish green color13…”

The effectiveness of basil extract on the mildew saprolegnia within in vitro

“…The results of the basil leaf phytochemical screening test conducted by Martiningsih & Suryanti (2017) using the water distillation method to produce Basil leaf essential oil contains flavonoids, saponins, terpenoids, and tannins. Meanwhile, in research by Kumalasari & Andiarna (2020) using the maceration method, it was found that 96% ethanol extract of basil leaves contained flavonoids, alkaloids, saponins, and tannins. Furthermore, Hasan, Raharjo & Ariyani (2016) added that the basil leaves contain substances as antibacterial and antifungal.…”