Uji Sitotoksisitas Ekstrak Ethanol 70 % Herba Ceplukan (Physalis angulata Linn.) Terhadap Sel WiDr Secara In Vitro

Abstract

AbstrakHerba ceplukan (Physalis angulata Linn) merupakan salah satu bahan alam yang digunakan masyarakat untuk pengobatan tradisional sebagai antikanker. Pada penelitian sebelumnya hasil pengujian ekstrak ethanol 70% herba ceplukan (Physalis angulata Linn.) terhadap Artemia salina Leach (larva udang) dengan metode Brine Shirmp Lethality Test (BSLT) diketahui memiliki sifat toksik dengan nilai LC 50 sebesar 39,63 µg/ml. Untuk itu dilakukan penelitian yang bertujuan mengetahui efek sitotoksisitas ekstrak ethanol 70% herba ceplukan dengan menentukan kadar yang menyebabkan 50% sel mati (LC 50 ) terhadap sel kanker usus WiDr secara in vitro. Herba ceplukan (Physalis angulata Linn) diekstraksi dengan cara maserasi dengan penyari ethanol 70%. Sel kanker usus WiDr diperlakukan dengan ekstrak ethanol 70% herba ceplukan dengan seri kadar yaitu 1000, 500, 250, 125, 62.5, 31.25, 15.125 dan 7.81 µg/ml selama 24 jam. Sebagai kontrol positif digunakan doksorubisin dengan seri kadar sebagai berikut: 2; 1; 0.5; 0.25; 0.12; 0.06; 0.03 dan 0 .01 µg/ml. Uji sitotoksisitas dilakukan dengan menginkubasi sel kanker usus WiDr dengan kepadatan akhir sel 2.10 4 sel / ml persumuran plat kultur. Uji sitotosisitas ini menggunakan metode perhitungan langsung dengan bantuan alat haemocytometer. Hasil penelitian menunjukkan bahwa ekstrak ethanol 70% herba ceplukan (Physalis angulata Linn.) mempunyai sitotoksisitas sebesar 86,84 μg/ml terhadap sel kanker usus WiDr yang di atas nilai indikator positif sebagai bahan bersifat sitotoksik yaitu sebesar > 30 μg/ml. Nilai tersebut juga sangat lebih tinggi bila dibandingkan dengan nilai LC 50 doksorubisin sebagai pembanding sebesar 0,113 µg/ml. AbstractCutleaf groundcherry (Physalis angulata) is a broadleaf weed commonly found in several plantations in Indonesia. This plant is a popular folk medicine used to treat cancer, leukemia, hepatitis and other diseases. Previous toxicity experiment using brine shrimp lethality test (BSLT) method indicated that the 70% ethanol fraction of cutleaf groundcherry fruits was toxic to the shrimp larvae with LC 50 value at 39,63 µg/ml. This experiment was done to investigate further the possible role of s as an anti cancer agent using WiDr colon cancer cell lines. Experiment was initiated by extracting the fruits using n-hexane by maceration. The macerate was then macerated further using 70% ethanol as solvent. The ethanol fraction was used in cytotoxicity assay using WiDr (colon cancer) cell lines. Cytotoxicity assay was done using direct counting method. Colon cancer WiDr cell lines was treated with 70% ethanol fraction of cutleaf groundcherry (Physalis angulata) using serial dilution of 1000, 500, 250, 125, 62.5, 31.25, 15.125 and 7.81 µg/ml for 24 hours of incubation. As a control positive doxorubicin was also tested on the colon cancer WiDr cell lines using serial dilution of 2; 1; 0.5; 0.25; 0.12; 0.06; 0.03 and 0 .01 µg/ml for 24 hours of incubation. The amount of living cells were observed and counted, death percentage was then determined and probit analysis was...

Combination Effect of Cecendet (Physalisangulata L.) Extract and Methylprednisolone in Reducing Inflammation and Improving Renal Functions in Pristane-induced Lupus Rat Models

“…Previous studies relating to the effects of cecendet herb stated that it is beneficial for anti-inflammation, immunomodulator, immunosuppressant, and cytotoxic. 5,8,9 In this study, the combination of cecendet extract and methylprednisolone can reduce inflammation compared to cecendet extract in 6 weeks of intervention. Measurement of inflammation inhibition in the combination of cecendet extract and methylprednisolone group described that effectiveness improved 19.5 times in week 6 compared to in week 3.…”

“…The extract works primarily on specific immune response by lowering immune response approaching normal condition, not by suppressing immune response as prednisone. [8][9][10] The animal models was induced by Pristane, an intraperitoneal hydrocarbon compound to induce lupus. The pristane can cause cell death through lymphoid cell apoptosis in the peritoneal cavity leading to autoimmune disease induction which is marked by the presence of positive antinuclear of antibody after a 4-week induction.…”

“…The pristane can cause cell death through lymphoid cell apoptosis in the peritoneal cavity leading to autoimmune disease induction which is marked by the presence of positive antinuclear of antibody after a 4-week induction. 8,9,11 The potential effect of cecendet as a supplement for SLE therapy is commonly associated with immunosuppressant as one of several standards for lupus therapies. Methylprednisolone is a main immunosuppressant used in particular dosages; high, moderate, and low as a treatment dosage.…”

Evaluation of Ethanolic Extracts of Mullaca (Physalis Angulata L.) Herbs for Treatment of Lupus Disease in Mice Induced Pristane

“…Physalis angulata known as mullaca, has been widely studied for its pharmacological activities such as antiinflammatory, immunosuppressive, cytotoxic, and also inhibition of organ rejection in transplantation [7][8][9][10] .…”

Characterization of Nutrition, Antioxidant Properties, and Toxicity of Physalis Angulata L. Plant Extract

“…The LC 50 values of ethanolic extract for ESC, ERC, ELC, EUF, and EFC were 2297 µg/ml, 2181 µg/ml, 2024 µg/ml, 1682 µg/ml, and 924 µg/ml, respectively. However, the ethanol extract of physalis has a low value when compared with the amount of value to other plants, except leaves or fruit part of physalis [32,33]. According to Kormin et al [34], variations in temperature during the process may affect poorly extracted LC 50 values.…”