Ultimate limits in high-pressure liquid chromatography

Halász, I.; Endele, R.; Asshauer, Jürgen

doi:10.1016/s0021-9673(00)99941-2

Cited by 252 publications

(67 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Besides the requirement for higher pressure and lower dead volume equipment in order to realise their full performance 8 , problems can arise due to frictional heating effects and shifts in retention that can be caused as a consequence of high pressure operation 9 . Further major innovations have taken place with the introduction of superficially porous particles or "core-shell" phases in which a porous layer of stationary phase surrounds a non-porous core that is impervious to solute penetration.…”

Section: General Considerationsmentioning

confidence: 99%

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Tanaka¹,

McCalley

2015

Anal. Chem.

153

View full text Add to dashboard Cite

Section: General Considerationsmentioning

confidence: 99%

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Tanaka¹,

McCalley

2015

Anal. Chem.

153

View full text Add to dashboard Cite

“…With UHPLC, frictional heating of the mobile phase [78] has been investigated. Small particles possess very low column permeability and thus generate a considerable amount of frictional heating under high pressure drops and high flow-rates.…”

Section: Frictional Heatingmentioning

confidence: 99%

Fast analysis in liquid chromatography using small particle size and high pressure

Nguyen

Guillarme

Rudaz

et al. 2006

J of Separation Science

303

164

View full text Add to dashboard Cite

ReviewFast analysis in liquid chromatography using small particle size and high pressureIn order to enhance chromatographic performances in terms of efficiency and rapidity, LC has recently evolved in the development of short columns packed with small particles (sub-2 lm) working at high pressures (A400 bar). This approach has been described 30 years ago according to the fundamental chromatographic equations. However, systems and columns compatible with such high pressures have been introduced in the market in 2004 only. Advantages of small particles working at high pressure will be discussed in terms of sensitivity, efficiency, resolution, and analysis time. Potential problems encountered with high pressure in terms of frictional heating and solvent compressibility will also be discussed even if systems working at a maximum pressure of 1000 bar are not influenced by these parameters and give reliable and reproducible results. Several applications will highlight the potential and interest of this new technology.

show abstract

“…As early as 1975, Halász et al [1] presumed theoretically that the smaller particles, e.g., particles having diameters of about 1 mm, could be employed and more efficient separations could be obtained. Higher column efficiency and faster analysis speed in HPLC have been achieved by using smaller particles that have smaller eddy diffusion and faster mass transfer rate [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Preparation and evaluation of C18‐bonded 1‐µm silica particles for pressurized capillary electrochromatography

Qu¹,

Xiao²,

Huang³

et al. 2006

Electrophoresis

View full text Add to dashboard Cite

Nonporous silica spheres (1 microm) were synthesized and bonded with octadecylsilane functionality. These stationary phase particles were packed electrokinetically into fused-silica capillaries with 100 microm id for a length of 20 cm, which was evaluated by using pressurized CEC (pCEC). The efficiency of the C18 RP column was characterized through the theoretical plates of thiourea, benzyl alcohol, toluene, styrene, and naphthalene. The effects of experimental parameters such as the applied voltage, sample size, pump flow rate, pH value and the concentration of the buffer solution, and the content of methanol in the mobile phase, on-column efficiency were evaluated. Column efficiency as high as 200 000 theoretical plates per meter for naphthalene was obtained with the optimal condition of 70% v/v methanol and 30% v/v of 10 mmol/L phosphate buffer (pH 7.8) at an applied voltage of 10 kV and a supplementary pressure of 500 psi.

show abstract

Ultimate limits in high-pressure liquid chromatography

Cited by 252 publications

References 13 publications

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Fast analysis in liquid chromatography using small particle size and high pressure

Preparation and evaluation of C18‐bonded 1‐µm silica particles for pressurized capillary electrochromatography

Contact Info

Product

Resources

About