Ultra-fast and universal detection of Gram-negative bacteria in complex samples based on colistin derivatives

Ryu, Jea Sung; Im, San Hae; Kang, Yoo Kyung; Kim, Yang Soo; Chung, Hyun Jung

doi:10.1039/c9bm01926j

Cited by 8 publications

(2 citation statements)

References 47 publications

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“…As shown in Fig. 2 b, the band for the ligated padlock probe is observed only when added with the target DNA for mecA, whereas the bands do not appear when target DNA is absence or when added with control target DNAs for other resistance genes NDM-1 and KPC [ 35 , 36 ]. Exo1 treatment of the padlock probe ligated by mecA target DNA resulted in a shift of the band to the (−) electrode, due to the decrease in size by removal of single-strand target DNA which used to be bound to the padlock probe [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

A Lateral Flow Assay for Nucleic Acid Detection Based on Rolling Circle Amplification Using Capture Ligand-Modified Oligonucleotides

et al. 2022

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We introduce a lateral flow assay (LFA) integrated with a modified isothermal nucleic acid amplification procedure for rapid and simple genetic testing. Padlock probes specific for the target DNA were designed for ligation, followed by rolling circle amplification (RCA) using capture ligand-modified oligonucleotides as primers. After hybridization with detection linker probes, the amplified target DNA is flowed through an LFA membrane strip for binding of gold nanoparticles as the substrate for colorimetric detection. We established and validated the “RCA-LFA” method for detection of mecA, the antibiotic resistance gene for methicillin-resistant Staphylococcus aureus (MRSA). The assay was optimized using various concentrations of primers and probes for RCA and LFA, respectively. The sensitivity was determined by performing RCA-LFA using various amounts of mecA target DNA, showing a detection limit of ~ 1.3 fmol. The specificity of the assay was examined using target DNAs for other resistance genes as the controls, which demonstrated positive detection signals only for mecA DNA, when added either individually or in combinations with the control targets. Furthermore, applying the RCA-LFA method using specifically designed probes for RNA-dependent RNA polymerase (RdRp) and receptor binding domain (RBD) gene for SARS-CoV-2, which demonstrated feasibility of the method for viral gene targets. The current method suggests a useful platform which can be universally applied for various nucleic acid targets, allowing rapid and sensitive diagnosis at point-of-care. Supplementary Information The online version contains supplementary material available at 10.1007/s13206-022-00080-1.

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Section: Resultsmentioning

confidence: 99%

A Lateral Flow Assay for Nucleic Acid Detection Based on Rolling Circle Amplification Using Capture Ligand-Modified Oligonucleotides

et al. 2022

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“…Bao et al synthesized probe 20 by combining polymyxin B with a TPE derivative, which can selectively detect photodynamic inactivated Gram-negative bacteria [ 72 ]. Due to the presence of polyamine groups in colistin E, Ryu et al synthesized probes 21 , 22 , and 23 in Figure 10 of colistin E and Cy3 for rapid clinical screening and stratification of Gram-negative bacterial infections [ 73 ]. Positively charged pyridine salts can effectively accumulate with negatively charged LPS by electrostatic forces.…”

Section: Identification Of the Cell Wall Components Of Pathogenic Bac...mentioning

confidence: 99%

Recent Progress in Identifying Bacteria with Fluorescent Probes

Wang

et al. 2022

Molecules

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The development of new techniques to rapidly and accurately detect bacteria has drawn continuous attention due to the potential threats posed by bacteria to human health and safety. Recently, a novel strategy based on fluorescent probes has drawn considerable interest for the detection of bacteria due to its high selectivity, fast response, and simple operation. In this review, we summarize the recent progress on fluorescent probes for the specific recognition and discrimination of Gram-negative and Gram-positive bacteria. In particular, we outline current design strategies, such as targeting of the differences in surface components, cell wall components, endogenous enzymes, surface charge, and hydrophobicity of various kinds of bacteria to develop various fluorescent sensors (organic small-molecule fluorescent probes, nanoprobes, and metal ion probes). We also emphasize the application of organic molecules in probe recognition elements. We hope that this review can stimulate this research area in bacterial detection and imaging in the future.

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Polydopamine Sensors of Bacterial Hypoxia via Fluorescence Coupling

Lee

Ryu

Kang

et al. 2020

Adv Funct Materials

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Biological catecholamines play critical physiological roles in various parts of the human body, namely, the skin and brain. In the skin, an oxygen‐contacting and oxygen‐abundant body part, catecholamine molecules are oxidatively polymerized, becoming melanin. In contrast, the brain is an oxygen‐demanding organ that suppresses catecholamine oxidation. Catecholamine oxidative polymerization, also known as polydopamine (or dopamine–melanin) formation, can be finely controlled by bacterial growth. Under exponential growth of Escherichia coli, a process that requires large amounts of oxygen, dopamine polymerization is significantly inhibited. In contrast, under steady‐state growth, polydopamine is formed due to the abundance of oxygen which is not actively consumed by E. coli. This polydopamine‐oxygen relationship is further demonstrated by using fluorescent dextran nanoparticles (FDNPs) as sensors, whose fluorescence is quenched by polydopamine formation. Thus, FDNP fluorescence can be precisely controlled by dopamine concentration, incubation time, and bacterial number. The cascade coupling of E. coli growth—oxygen level—polydopamine—fluorescence can also be used to detect the antibiotic‐resistant bacteria, New Delhi metallo‐beta‐lactamase 1‐positive (NDM1+) E. coli. This method not only uncovers the unique role played by biological catecholamine in a living system, but also presents a diagnostic assay for detecting bacterial growth and antibiotic susceptibility.

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Ultra-fast and universal detection of Gram-negative bacteria in complex samples based on colistin derivatives

Abstract: A rapid and universal assay for detection of Gram-negative bacteria was developed using a fluorescent derivative of colistin. Labeling is achieved within 10 min in various bacteria relevant to hospital-acquired infections in complex samples.

Cited by 8 publications

References 47 publications

A Lateral Flow Assay for Nucleic Acid Detection Based on Rolling Circle Amplification Using Capture Ligand-Modified Oligonucleotides

A Lateral Flow Assay for Nucleic Acid Detection Based on Rolling Circle Amplification Using Capture Ligand-Modified Oligonucleotides

Recent Progress in Identifying Bacteria with Fluorescent Probes

Polydopamine Sensors of Bacterial Hypoxia via Fluorescence Coupling

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