Ultra-performance liquid chromatography coupled to time of flight mass spectrometry (UPLC–TOF): A novel tool for multiresidue screening of veterinary drugs in urine

Kaufmann, Anton; Butcher, Patrick; Maden, Kathryn; Widmer, Mirjam

doi:10.1016/j.aca.2006.10.026

Cited by 132 publications

(60 citation statements)

References 7 publications

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“…Thus, a key question is whether the medium mass resolving power provided by time-of-flight (TOF) instruments is sufficient to enable the detection and accurate mass measurement of residues at low ng/g level in complex sample matrices. The utility of full-scan LC-TOFMS for the comprehensive screening of urine samples for drugs [3,4] and doping agents [5], crops for pesticide residues [6], and confirmation of antibiotics and fungicides in salmon [7] was demonstrated. Comparison of theoretical and measured isotopic patterns might be a useful additional tool to accurate mass determination and facilitates the identification process [8].…”

mentioning

confidence: 99%

Full-scan accurate mass selectivity of ultra-performance liquid chromatography combined with time-of-flight and orbitrap mass spectrometry in hormone and veterinary drug residue analysis

Heeft¹,

Bolck²,

Beumer³

et al. 2009

J. Am. Soc. Mass Spectrom.

128

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The applicability of ultra-performance liquid chromatography (UPLC) combined with fullscan accurate mass time-of-flight (TOF) and Orbitrap mass spectrometry (MS) to the analysis of hormone and veterinary drug residues was evaluated. Extracts from blank bovine hair were fortified with 14 steroid esters. UPLC-Orbitrap MS performed at a resolving power of 60,000 (FWHM) enabled the detection and accurate mass measurement (Ͻ3 ppm error) of all 14 steroid esters at low ng/g concentration level, despite the complex matrix background. A 5 ppm mass tolerance window proved to be essential to generate highly selective reconstructed ion chromatograms (RICs) having reduced background from the hair matrix. UPLC-Orbitrap MS at a lower resolving power of 7500 and UPLC-TOFMS at mass resolving power 10,000 failed both to detect all of the steroid esters in hair extracts owing to the inability to mass resolve analyte ions from co-eluting isobaric matrix compounds. In a second application, animal feed extracts were fortified with coccidiostats drugs at levels ranging from 240 to 1900 ng/g. UPLC-Orbitrap MS conducted at a resolving power of 7500 and 60,000 and UPLC-TOFMS detected all of the analytes at the lowest investigated level. Thanks to the higher analyte-to-matrix background ratio, the utilization of very narrow mass tolerance windows in the RIC was not required. This study demonstrates that even when the targeted sample preparation from conventional LC-MS/MS is applied to UPLC with full-scan accurate mass MS, false compliant (false negative) results can be obtained when the mass resolving power of the MS is insufficient to separate analyte ions from isobaric co-eluting sample matrix ions. The current trend towards more generic and less selective sample preparation is expected to aggravate this issue further. (J Am Soc Mass Spectrom 2009, 20, 451-463)

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mentioning

confidence: 99%

Full-scan accurate mass selectivity of ultra-performance liquid chromatography combined with time-of-flight and orbitrap mass spectrometry in hormone and veterinary drug residue analysis

Heeft¹,

Bolck²,

Beumer³

et al. 2009

J. Am. Soc. Mass Spectrom.

128

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“…It appears that columns packed with sub-2 lm particles and working under ultra high pressure (UHPLC) have emerged as an attractive approach for high throughput screening, providing faster separations without compromising chromatographic resolution or MS sensitivity [6,7]. Therefore, UHPLC-MS-MS strategy is often employed, particularly in the fields of bioanalysis (determination of various drugs and metabolites in biological fluids) [8][9][10] or multi-residue screening (determination of numerous contaminants such as pesticides, drugs or doping agents in complex matrices) [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Screening of Drugs of Abuse in Urine by Supported Liquid–Liquid Extraction and UHPLC Coupled to Tandem MS

Maquille

Guillarme

Rudaz

et al. 2009

Chroma

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A qualitative method, involving supported liquid-liquid extraction (SLE) and ultra high pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS-MS), was developed for the rapid tentative identification of various drugs of abuse in urine. In this study, 28 drugs and metabolites were covered by the screening procedure. Before analysis, urine samples were extracted by SLE and good extraction recoveries were obtained for most investigated compounds. The UHPLC strategy was then selected for the rapid separation of amphetamines, cocaine, opiates and related compounds in urine. Using columns packed with sub-2 lm particles, analysis time was reduced down to 2 min, while maintaining acceptable performance. Finally, the detection was by tandem MS operating in the single reaction monitoring (SRM) mode. The most intense transition was selected for the different drugs and SRM dwell times set at 5 ms, to maintain sufficient data points across the narrow UHPLC peaks. The tentative identification of the drugs of interest, including amphetamines, opiates and cocaine, was based on both, retention times and mass spectrometry information. With the proposed method, limits of detection were estimated at about 1 ng mL -1 and the applicability was assessed by successfully analyzing several samples of drug abusers. Finally, this study demonstrates the potential of UHPLC coupled to tandem MS for the rapid screening of drugs of abuse in urine.

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“…Além dos trabalhos descritos acima, outros foram realizados empregando CLUE, como, por exemplo, determinação de lactonas, 23 to- xinas marinhas, 45 cianobactérias, 46 drogas veterinárias, 47 saponinas, 12,48 enzimas 49 e metabonomas, 50 o que comprova que a cromatografia líquida de ultra eficiência vem sendo avaliada e empregada em diversas áreas.…”

Section: Aplicações Da Clueunclassified

O estado da arte da cromatografia líquida de ultra eficiência

Maldaner

Jardim

2009

Quím. Nova

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Recebido em 8/4/08; aceito em 14/7/08; publicado na web 5/12/08 THE STATE OF ART OF ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY. Ultra Performance Liquid Chromatography (UPLC) retains the same principles as High Performance Liquid Chromatography (HPLC), but uses 1-2.1 mm i.d. columns with sub-2 µm particles. It is considered the newest advance in analytical separation science. The use of these small particles with mobile phases at high linear velocities increases resolution and detectability and decreases analysis time. Thus, the analyses are faster, the solvent volume is smaller, the efficiency is higher and the detectability is 2-3 times higher when compared with HPLC analysis.

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Ultra-performance liquid chromatography coupled to time of flight mass spectrometry (UPLC–TOF): A novel tool for multiresidue screening of veterinary drugs in urine

Cited by 132 publications

References 7 publications

Full-scan accurate mass selectivity of ultra-performance liquid chromatography combined with time-of-flight and orbitrap mass spectrometry in hormone and veterinary drug residue analysis

Full-scan accurate mass selectivity of ultra-performance liquid chromatography combined with time-of-flight and orbitrap mass spectrometry in hormone and veterinary drug residue analysis

High-Throughput Screening of Drugs of Abuse in Urine by Supported Liquid–Liquid Extraction and UHPLC Coupled to Tandem MS

O estado da arte da cromatografia líquida de ultra eficiência

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