Ultracytochemical studies on fat absorption by choline-deficient rats.

Takahashi, Yukari; Mizunuma, Toshimi; Kawaguchi, Yasuo

doi:10.1267/ahc.15.90

Cited by 11 publications

(9 citation statements)

References 27 publications

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“…Panel C: effects of treatment (P = 0.01), BCS (P = 0.02), and interaction between treatment and BCS (P = 0.75). lipids in part because of a small content of phosphatidylcholine in the intestine (da Silva et al, 2015), which impairs the secretion of chylomicron into lymph (Takahashi et al, 1982). In nonlactating dairy cows after feed restriction and subsequent fat challenge, RPC increased mean plasma concentration of triacylglycerol compared with nonsupplemented controls (Zenobi et al, 2018b), thereby suggesting that choline enhances the absorptive capacity of the gastrointestinal tract.…”

Section: Discussionmentioning

confidence: 99%

Responses to rumen-protected choline in transition cows do not depend on prepartum body condition

Bollatti

Zenobi

Barton³

et al. 2020

Journal of Dairy Science

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Section: Discussionmentioning

confidence: 99%

Responses to rumen-protected choline in transition cows do not depend on prepartum body condition

Bollatti

Zenobi

Barton³

et al. 2020

Journal of Dairy Science

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“…Likewise, the jejunal tissue of lactating rats contained 2.4 times more TAG and 19% less PC if fed a diet deficient in choline (da Silva et al, 2015). One of the more remarkable ultracytochemical changes in absorptive cells of the small intestine in rats fed a choline-free diet was the lowered activity of Ca-ATPase in the terminal web and lower activities of several ATPases, highlighting the negative effect of choline deficiency on intracellular transport and discharge of chylomicrons (Takahashi et al, 1982b).…”

Section: Fat Loadingmentioning

confidence: 97%

Feeding increasing amounts of ruminally protected choline decreased fatty liver in nonlactating, pregnant Holstein cows in negative energy status

Zenobi

Scheffler

Zuniga

et al. 2018

Journal of Dairy Science

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The objectives were to determine the optimal feeding amount of choline in a ruminally protected form to reduce the triacylglycerol (TAG) concentration in liver and to increase TAG in blood plasma of dairy cows. Pregnant, nonlactating multiparous Holstein cows (n = 77) were blocked by body condition score (3.59 ± 0.33) and assigned to treatment at 64 ± 10 d before calculated calving date. Dietary treatments were top-dressing of 0, 30, 60, 90, or 120 g/d of ruminally protected choline (RPC; Balchem Corp., New Hampton, NY) ions to supply the equivalent of 0, 6.5, 12.9, 19.4, and 25.8 g/d of choline ions. Diets were formulated to exceed nutrient requirements for maintenance and pregnancy and fed in ad libitum amounts for the first 5 d. From d 6 to 15, cows were restricted to consume approximately 31% of their net energy requirements to simulate early lactating cows in negative energy balance. Methionine intake was maintained throughout each 15-d period. Liver was biopsied at 5 and 14 d and analyzed for TAG and glycogen. Blood was sampled on d 5 and 14 and plasma analyzed for glucose, insulin, cholesterol, β-hydroxybutyrate, long-chain fatty acids, and haptoglobin. On d 14, a mixture of saturated long-chain fatty acids, ground corn, and dried molasses (50:37:13) was offered (908 g, as-is basis) 10 h after the single daily feeding. Blood samples were collected for 19 h and plasma analyzed for TAG and cholesterol to assess apparent absorption of dietary fat. Mean dry matter intake and energy balance decreased from means of 9.5 to 3.3 kg/d and from 0.6 to -9.2 Mcal of net energy for lactation/d during the ad libitum and restricted feeding periods, respectively. Plasma concentrations of the lipid-soluble choline biomolecules, namely total phosphatidylcholines, total lysophosphatidylcholines, and sphingomyelin, increased with choline supplementation. Feed restriction increased plasma concentrations of β-hydroxybutyrate and free long-chain fatty acids, whereas those of glucose, insulin, and total cholesterol decreased. During feed restriction, concentration of hepatic TAG and plasma haptoglobin decreased linearly, whereas concentration of hepatic glycogen tended to increase quadratically with increasing intake of RPC. After fat supplementation, mean plasma concentration of TAG increased by an average of 21% with intake of RPC ions, peaking at intakes of ≥6.5 g/d of RPC ion. In summary, feeding RPC ions to cows in negative energy balance had increasing lipotropic effects on the liver when consumed up to 25.8 g/d, whereas feeding only 6.5 g/d increased concentrations of hepatic glycogen and TAG in the blood.

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“…Other studies have shown that choline deficiency in adult male rats reduces lipoprotein secretion into the lymph and increases the volume and number of lipid droplets in absorptive cells after infusion of corn oil [19,20] body weight were recorded regularly throughout lactation. Two of the original ten dams in the CD group had to be killed prior to the end of the experiment due to significant weight loss.…”

Section: Accepted Manuscriptmentioning

confidence: 99%