Ultrafiltration of blood proteins by experimental polyamide membranes

Ramos, Ana M. González; Soriano, Enrique

doi:10.1007/s004490050508

Cited by 10 publications

(7 citation statements)

References 3 publications

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“…All the buffer solutions were adjusted to desired pH values by mixing different amounts of potassium dihydrogen phosphate (KH2PO4) and disodium hydrogen phosphate (Na2HPO4) (both from Fluka) in Milli-Q water and using these solutions within 3 days of preparation. Phosphate buffer saline solutions maintaining different external pH with low ionic strength (I ¼ 0.01 M) were used in the experiments 38 .…”

Section: Methodsmentioning

confidence: 99%

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Biomimetic block copolymer particles with gated nanopores and ultrahigh protein sorption capacity

et al. 2014

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The design of micro-or nanoparticles that can encapsulate sensitive molecules such as drugs, hormones, proteins or peptides is of increasing importance for applications in biotechnology and medicine. Examples are micelles, liposomes and vesicles. The tiny and, in most cases, hollow spheres are used as vehicles for transport and controlled administration of pharmaceutical drugs or nutrients. Here we report a simple strategy to fabricate microspheres by block copolymer self-assembly. The microsphere particles have monodispersed nanopores that can act as pH-responsive gates. They contain a highly porous internal structure, which is analogous to the Schwarz P structure. The internal porosity of the particles contributes to their high sorption capacity and sustained release behaviour. We successfully separated similarly sized proteins using these particles. The ease of particle fabrication by macrophase separation and self-assembly, and the robustness of the particles makes them ideal for sorption, separation, transport and sustained delivery of pharmaceutical substances.

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Section: Methodsmentioning

confidence: 99%

“…Size-based separation of proteins can be carried out by ultrafiltration membrane systems when the proteins are significantly different in size 38,39 . However, separation of similarly sized proteins with the commercially available membranes is not possible 40 .…”

Section: Preparation and Characterization Of Nanoporous Particlesmentioning

confidence: 99%

Biomimetic block copolymer particles with gated nanopores and ultrahigh protein sorption capacity

et al. 2014

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“…Separation of proteins can be effectively carried out with membrane separation processes. − In general, proteins are easily separated with porous membranes if the proteins have significantly different molecular sizes. However, membrane separation of similar size proteins, such as hemoglobin and albumin, is more difficult, and these separations have received renewed interest.…”

Section: Introductionmentioning

confidence: 99%

Protein Transport in Nanoporous Membranes Modified with Self-Assembled Monolayers of Functionalized Thiols

2002

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Control of external pH and ionic strength is used to separate proteins with surface-modified, nanoporous polycarbonate track etched (PCTE) membranes. The porous PCTE membranes were modified with monolayers of self-assembled thiols (HSC10H20COOH) on electroless gold. The hydraulic radius of the pores in the surface-modified membranes was 8.7 nm. Two proteins of nearly identical molecular weight, bovine serum albumin (BSA) and bovine hemoglobin (BHb), were used as the permeants. The fluxes of BSA and BHb through the membranes show maximum values at the isoelectric points (pI) of the proteins. At pH values above and below the pI, charge interactions between the proteins, their counterions, and the pore surface leads to a decrease in flux. The imposition of a difference in ionic strength across the membrane causes osmotic flow and leads to a significant increase in the protein fluxes and an enhancement of the selectivity of BSA over BHb. In protein separation experiments, the BSA and BHb fluxes are nearly 3 times larger than those observed with no ionic strength difference.

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“…Existing processes for bioseparations using conventional resin-based chromatography are time-consuming and expensive. , Newly developed membrane separation processes offer substantial economic, environmental (less energy consumption), and safety benefits. , Micro/nanofabricated membranes with various pore sizes, lengths, morphologies, and densities have been synthesized from diverse inorganic, organic, and composite materials. − Studies of nanostructured membranes have demonstrated their great potential in biomolecule separation applications. − Size-based separation of proteins can be carried out by ultrafiltration membrane systems when the proteins have significantly different molecular sizes. − However, separation of similar-sized proteins with current commercially available membranes is not possible . Recently, Striemer et al .…”

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confidence: 99%

Selective Separation of Similarly Sized Proteins with Tunable Nanoporous Block Copolymer Membranes

et al. 2012

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An integral asymmetric membrane was fabricated in a fast and one-step process by combining the self-assembly of an amphiphilic block copolymer (PS-b-P4VP) with nonsolvent-induced phase separation. The structure was found to be composed of a thin layer of densely packed highly ordered cylindrical channels with uniform pore sizes perpendicular to the surface on top of a nonordered sponge-like layer. The as-assembled membrane obtained a water flux of more than 3200 L m–2 h–1 bar–1, which was at least an order of magnitude higher than the water fluxes of commercially available membranes with comparable pore sizes, making this membrane particularly well suited to size-selective and charge-based separation of biomolecules. To test the performance of the membrane, we conducted diffusion experiments at the physiological pH of 7.4 using bovine serum albumin (BSA) and globulin-γ, two proteins with different diameters but too close in size (2-fold difference in molecular mass) to be efficiently separated via conventional dialysis membrane processes. The diffusion rate differed by a factor of 87, the highest value reported to date. We also analyzed charge-based diffusive transport and separation of two proteins of similar molecular weight (BSA and bovine hemoglobin (BHb)) through the membrane as a function of external pH. The membrane achieved a selectivity of about 10 at pH 4.7, the isoelectric point (pI) of BSA. We then positively charged the membrane to improve the separation selectivity. With the modified membrane BSA was completely blocked when the pH was 7.0, the pI of BHb, while BHb was completely blocked at pH 4.7. Our results demonstrate the potential of our asymmetric membrane to efficiently separate biological substances/pharmaceuticals in bioscience, biotechnology, and biomedicine applications.

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Ultrafiltration of blood proteins by experimental polyamide membranes

Cited by 10 publications

References 3 publications

Biomimetic block copolymer particles with gated nanopores and ultrahigh protein sorption capacity

Biomimetic block copolymer particles with gated nanopores and ultrahigh protein sorption capacity

Protein Transport in Nanoporous Membranes Modified with Self-Assembled Monolayers of Functionalized Thiols

Selective Separation of Similarly Sized Proteins with Tunable Nanoporous Block Copolymer Membranes

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