Ultrasensitive caspase-3 activity detection using an electrochemical biosensor engineered by gold nanoparticle functionalized MCM-41: Its application during stem cell differentiation

Khalilzadeh, Balal; Charoudeh, Hojjatollah Nozad; Shadjou, Nasrin; Mohammad‐Rezaei, Rahim; Omidi, Yadollah; Velaei, Kobra; Aliyari, Z; Rashidi, Mohammad-Reza

doi:10.1016/j.snb.2016.03.043

Cited by 54 publications

(12 citation statements)

References 49 publications

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“…In continuing our previous studies, 18,26 in this report, using three strategies of loading more Aspartic acid-Glutamic acid-Valine-Aspartic acid (DEVD) modified peptide as caspase-3 substrate on the electrode surface through AuNPs-Th-GO platform, involvement of more HRP molecules in the reaction through the use of streptavidin coated magnetic beads, and finally the use of luminol/HRP/H 2 O 2 ECL system, we could quantify caspase-3 activity with the lower limit of quantification (LLOQ) of 0.5 fM. As a protease, caspase-3 is able to cleavage DEVD, and this peptide sequence has been used by many scientists for quantification of caspase-3 activity.…”

Section: Introductionsupporting

confidence: 68%

“…26 The reason of this approach is high surface area and electrical conductivity of RGO than MCM-41 and merging of both electrochemical and luminescence methods. Additionally, both amplification strategies for signal enhancing were used in this luminol-based ECL assay (reporter amplification using streptavidin coated magnetic beads and electrode modifier amplification mode by AuNPs-RGO).…”

Section: Discussionmentioning

confidence: 99%

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Reduced graphene oxide decorated with gold nanoparticle as signal amplification element on ultra-sensitive electrochemiluminescence determination of caspase-3 activity and apoptosis using peptide based biosensor

Khalilzadeh¹,

Shadjou²,

Afsharan³

et al. 2016

Bioimpacts

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Introduction:Growing demands for ultrasensitive biosensing have led to the development of numerous signal amplification strategies. In this report, a novel electrochemiluminescence (ECL) method was developed for the detection and determination of caspase-3 activity based on reduced graphene oxide sheets decorated by gold nanoparticles as signal amplification element and horseradish peroxidase enzyme (HRP) as ECL intensity enhancing agent. Methods: The ECL intensity of the luminol was improved by using the streptavidin coated magnetic beads and HRP in the presence of hydrogen peroxide. The cleavage behavior of caspase-3 was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques using biotinylated peptide (DEVD containing peptide) which was coated on reduced graphene oxide decorated with gold nanoparticle. The surface modification of graphene oxide was successfully confirmed by FTIR, UV-vis and x-ray spectroscopy. Results: ECL based biosensor showed that the linear dynamic range (LDR) and the lower limit of quantification (LLOQ) were 0.5-100 and 0.5 femtomolar (fM), respectively. Finally, the performance of the engineered peptide based biosensor was validated in the A549 cell line as real samples. Conclusion: The prepared peptide based biosensor could be considered as an excellent candidate for early detection of apoptosis, cell turnover, and cancer related diseases.

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Section: Introductionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Reduced graphene oxide decorated with gold nanoparticle as signal amplification element on ultra-sensitive electrochemiluminescence determination of caspase-3 activity and apoptosis using peptide based biosensor

Khalilzadeh¹,

Shadjou²,

Afsharan³

et al. 2016

Bioimpacts

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“…The sensitivity and the detection limit of these biosensors depend on the number of enzyme molecules, 14,15 and thus, immobilization of the enzyme on the surface of electrodes is a crucial step for the performance of the biosensors. In order to rmly immobilize the enzyme, numerous advanced materials such as carbon nanotubes, [16][17][18][19] gold nanoparticles, [20][21][22] and porous materials 23,24 have been introduced for the construction of enzyme-loaded matrixes. The integration of enzyme and porous materials greatly promoted the sensitivity, stability and detection limit of enzyme biosensors.…”

Section: Introductionmentioning

confidence: 99%

A novel biosensor based on ball-flower-like Cu-hemin MOF grown on elastic carbon foam for trichlorfon detection

et al. 2018

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“…The thiol‐ended copolymer has a strong attraction towards metal nanoparticle like gold nanospheres (GNSs) via the creation of Au–S bond. In new year's, polymer modified GNSs have been attained a tremendous attention in bioanalytical (eg, biosensors) and biomedical (eg, diagnosis and drug delivery) fields. This intense attention created from biological features as well as superior physicochemical of GNSs including low cytotoxicity, good biocompatibility, excellent chemical stability, and enhance the transfection efficiency …”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of gold nanospheres‐cored pH‐sensitive thiol‐ended triblock copolymer: A smart drug delivery system for cancer therapy

Mahmoodzadeh

Hosseinzadeh

Jannat

et al. 2019

Polymers for Advanced Techs

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Conventional chemotherapy suffers lack of multidrug resistance (MDR), lack of bioavailability, and selectivity. Nano‐sized drug delivery systems (DDS) are developing aimed to solve several limitations of conventional DDS. These systems have been offered for targeting tumor tissue owing to enhanced long circulation time, drug solubility, their retention effect, and improved permeability. As a result, the aim of this project was the design and development of DDS for biomedical applications. For this purpose, gold nanospheres (GNSs) covered by pH‐sensitive thiol‐ended triblock copolymer [poly(methacrylic acid) ‐b‐poly(acrylamide) ‐b‐poly(ε‐caprolactone)‐SH; PMAA‐b‐PAM‐b‐PCL‐SH] for delivery of anticancer drug doxorubicin (DOX). The chemical structures of triblock copolymer were investigated by proton nuclear magnetic resonance (1H NMR) and Fourier transform infrared (FTIR) spectroscopies. 1H NMR spectroscopy and gel permeation chromatography (GPC) were used for calculating the molecular weights of each part in the nanocarrier. The success of coating, GNSs with triblock copolymer was considered by means of dynamic light scattering (DLS), FTIR, ultraviolet‐visible (UV‐Vis), and transmission electron microscopy (TEM) measurement. The pH‐responsive drug release ability, (DOX)‐loading capacity, biocompatibility, and in vitro cytotoxicity effects of the nanocarriers were also studied. As a result, it is expected that the synthesized GNSs@polymer‐DOX considered as a potential application in nanomedicine demand like smart drug delivery, imaging, and chemo‐photothermal therapy.

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Ultrasensitive caspase-3 activity detection using an electrochemical biosensor engineered by gold nanoparticle functionalized MCM-41: Its application during stem cell differentiation

Cited by 54 publications

References 49 publications

Reduced graphene oxide decorated with gold nanoparticle as signal amplification element on ultra-sensitive electrochemiluminescence determination of caspase-3 activity and apoptosis using peptide based biosensor

Reduced graphene oxide decorated with gold nanoparticle as signal amplification element on ultra-sensitive electrochemiluminescence determination of caspase-3 activity and apoptosis using peptide based biosensor

A novel biosensor based on ball-flower-like Cu-hemin MOF grown on elastic carbon foam for trichlorfon detection

Fabrication and characterization of gold nanospheres‐cored pH‐sensitive thiol‐ended triblock copolymer: A smart drug delivery system for cancer therapy

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