Ultrasensitive Detection of ctDNA by Target‐Mediated In Situ Growth of DNA Three‐Way Junction on the Electrode

Chen, Xifeng; Tang, Yuguo; Yan, Rui

doi:10.1002/celc.201901657

Cited by 8 publications

(3 citation statements)

References 27 publications

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“…The 5′ end single-stranded region is labeled with methylene blue (MB), which provide intense electrochemical response amplified by tris(2-carboxyethyl)phosphine hydrochloride (TCEP). [23] The middle region is another hairpin structure, which can be opened by complete Probe b on the surface AuNPs by hybridization. Meanwhile, the formed duplex can be recognized by Nb.BbvCI NEase and Probe c is thus cleaved, [24] releasing the 5′ end part with MB.…”

Section: Sensing Principlementioning

confidence: 99%

DNA Walking and Rolling Nanomachine for Electrochemical Detection of miRNA

Miao

Tang

2020

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AbstractmiRNAs, a class of endogenous noncoding RNAs, are involved in many crucial biological processes, which have emerged as a new set of biomarkers for disease theranostics. Exploring efficient signal amplification strategy is highly desired to pursue a highly sensitive miRNA biosensing platform. DNA nanotechnology shows great promise in the fabrication of amplified miRNA biosensors. In this work, a novel DNA walking and rolling nanomachine is developed for highly sensitive and selective detection of miRNA. Particularly, this approach programs two forms of dynamic DNA nanomachines powered by corresponding enzymes, which are well integrated. It is able to achieve a limit of detection as low as 39 × 10−18 m, along with excellent anti‐interfering performance and clinical applications. In addition, by designing pH‐controlled detachable intermolecular DNA triplex, the main sensing elements can be conveniently reset, which fulfills the requirements of point‐of‐care profiling of miRNA. The high consistency between the proposed approach and quantitative real‐time polymerase chain reaction validates the robustness and reliability. Therefore, it is anticipated that the DNA walking and rolling nanomachine has attractive application prospects in miRNA assay for biological researches and clinical diagnosis.

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Section: Sensing Principlementioning

confidence: 99%

DNA Walking and Rolling Nanomachine for Electrochemical Detection of miRNA

Miao

Tang

2020

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“…In addition to the evaluation of potential measurement and signal amplification schemes, the importance of the underlying electrode has also been investigated through the use of e.g., nanostructured gold electrodes [18]. What these advances have so far reported are complex assays involving the use of modified oligonucleotides [19], formation of branched structures upon DNA recognition [20], molecular switches [21], nanostructured electrode modifications [18], etc., therefore ultimately having a limited chance of manufacture and clinical uptake. The current state-of-the-art in terms of general nucleic acid liquid biopsies [22] and electrochemical detection of ctDNA biomarkers [23] have been well-reviewed recently.…”

Section: Introductionmentioning

confidence: 99%

Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids

et al. 2020

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It is well-known that two major issues, preventing improved outcomes from cancer are late diagnosis and the evolution of drug resistance during chemotherapy, therefore technologies that address these issues can have a transformative effect on healthcare workflows. In this work we present a simple, low-cost DNA biosensor that was developed specifically to detect mutations in a key oncogene (KRAS). The sensor employed was a screen-printed array of carbon electrodes, used to perform parallel measurements of DNA hybridisation. A DNA amplification reaction was developed with primers for mutant and wild type KRAS sequences which amplified target sequences from representative clinical samples to detectable levels in as few as twenty cycles. High levels of sensitivity were demonstrated alongside a clear exemplar of assay specificity by showing the mutant KRAS sequence was detectable against a significant background of wild type DNA following amplification and hybridisation on the sensor surface. The time to result was found to be 3.5 h with considerable potential for optimisation through assay integration. This quick and versatile biosensor has the potential to be deployed in a low-cost, point-of-care test where patients can be screened either for early diagnosis purposes or monitoring of response to therapy.

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“…Biosensors are devices that convert biological signals into electrical, optical, or thermodynamic signals. They can measure the biochemical signals of entire cells by physical devices such as fluorescent biosensors, optical transducers, surface-enhanced Raman scattering (SERS), localized surface plasmon resonance (LSPR), and electrochemical biosensors [15][16][17][18][19][20][21][22]. Thus, the biosensors offer a more conducive approach to detect ctDNA because of their convenience and precision [23][24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Entropy-driven strand displacement reaction for ultrasensitive detection of circulating tumor DNA based on upconversion and Fe3O4 nanocrystals

et al. 2021

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Early detection of cancer biomarkers applied in real-time disease diagnosis and therapies can increase the survival rate of patients. Circulating tumor DNA (ctDNA) as a typical cancer biomarker plays a great role in the process of tumor disease monitoring, especially in early diagnosis. Unfortunately, most ctDNA detection systems have not been widely used due to their low sensitivity, poor specificity, and high cost. Herein, we developed an alternative ctDNA detection system to present the levels of ctDNA by recording the fluorescence signals of the system containing upconversion nanoparticles (UCNPs), Fe 3 O 4 , and entropy-driven strand displacement reaction. The method has a practical sensitivity with a wide linear range from 100 amol L −1 to 1 nmol L −1 and a low detection limit of 1.6 amol L −1 . Furthermore, the system demonstrates a practical application in mouse blood serum samples and meets the requirements for rapid, sensitive, specific, and economical diagnosis of cancers. Thus, this ctDNA detection system may have great potential for ctDNA detection and clinical diagnosis.

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Ultrasensitive Detection of ctDNA by Target‐Mediated In Situ Growth of DNA Three‐Way Junction on the Electrode

Cited by 8 publications

References 27 publications

DNA Walking and Rolling Nanomachine for Electrochemical Detection of miRNA

DNA Walking and Rolling Nanomachine for Electrochemical Detection of miRNA

Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids

Entropy-driven strand displacement reaction for ultrasensitive detection of circulating tumor DNA based on upconversion and Fe3O4 nanocrystals

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