Ultrasensitive Glutathione Detection Based on Lucigenin Cathodic Electrochemiluminescence in the Presence of MnO₂ Nanosheets

Abstract

Glutathione (GSH) is a crucial antioxidant produced endogenously and plays key roles in biological systems. It is vitally important to design simple, selective, and sensitive methods to sense GSH and monitor changes of GSH concentration. In this work, the cathodic electrochemiluminescence (ECL) of lucigenin in the presence of MnO2 nanosheets at a glassy carbon electrode was utilized for GSH detection. GSH can reduce MnO2 nanosheets into Mn(2+) which can obviously inhibit the ECL of lucigenin. The ECL inhibition efficiencies increase linearly with the concentrations of glutathione in the range of 10 to 2000 nM. The detection limit for GSH measurement is 3.7 nM. This proposed method is highly sensitive, selective, simple, fast, and cost-effective. Moreover, this approach can detect GSH in human serum samples with excellent recoveries, which indicates its promising application under physiological conditions.

Nanozyme applications in biology and medicine: an overview

“…Additionally, the complex can protect the algae against biofilm formation. Towards the biocidal (anti-bacterial) potential of the peroxidases [62], another application of vanadium peroxidase is neutralization of biofilms by mimic vanadium halo peroxidases [61]. Nanowires had catalytic activity towards peroxidase substrates (such as ABTS and TMB) in the presence of H 2 O 2 as demonstrated in a study from Trammel's group.…”

“…H 2 O 2 detection is useful in many fields such as biology, medicine, environmental protection and the food industry [61]. There is a higher efficient way to Glucose detection via glucose oxidase (GOx) [62].…”

“…Liu et al showed that BSA-stabilized Mno þ2 can be used instead of HRP enzyme and in the detection of goat anti-human in immunoassay technic as a colorimetric application [60]. In a study, MnO 2 nanosheet was used for detection of Glutathione via Electrochemical luminescence [61].…”

Detection of Ascorbic Acid Using Green Synthesized Carbon Quantum Dots

“…Ascorbic acid (vitamin C, AA) plays a crucial role as an essential nutrient and antioxidant in the human body, which exists only in food and drugs and cannot be synthesized by human beings themselves. The abnormal concentration of AA can be related to various kinds of diseases including scurvy, mental disorder, cancer, cold, AIDS, and digestive disorder [1][2][3][4][5]. In addition, AA has been found to participate in a lot of life processes, such as cell division, iron absorption, acceleration of collagen synthesis, melanogenesis inhibition, and metabolism [6][7][8][9].…”

A GSH Fluorescent Probe with a Large Stokes Shift and Its Application in Living Cells

“…Familiar methods to detect GSH mainly include electrochemistry [12], colorimetry [13], surface-enhanced Raman scattering [14], magnetic resonance imaging [15], mass spectrometry [16], electrochemical luminescence [17], and fluorescence spectroscopy [18]. Among those detection methods, fluorescent analysis is a much simpler, more efficient and lower destructive method and detecting limits, which can be applied in living cells and even in vivo to imaging bio-thiols.…”