Ultrasensitive label-free electrochemical immunosensor based on PVA-co-PE nanofibrous membrane for the detection of chloramphenicol residues in milk

El-Moghazy, Ahmed Y.; Zhao, Cunyi; Istamboulié, Georges; Amaly, Noha; Si, Yang; Noguer, Thierry; Sun, Gang

doi:10.1016/j.bios.2018.07.025

Cited by 85 publications

(28 citation statements)

References 47 publications

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“…PVA-co-PE nanofibrous membrane was fabricated according to the literature [16,17]. PVA-co-PE (Mn = 90,000) was added into a mixture of isopropanol and water (weight ratio 7:3) with stirring at 80°C for 2 h to prepare electrospinning solutions.…”

Section: Fabrication Of Pva-co-pe Nanofibrous Membranesmentioning

confidence: 99%

Development of Improved Double-Nanobody Sandwich ELISAs for Human Soluble Epoxide Hydrolase Detection in Peripheral Blood Mononuclear Cells of Diabetic Patients and the Prefrontal Cortex of Multiple Sclerosis Patients

Morisseau

McReynolds

et al. 2020

Anal. Chem.

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Enzyme-linked immunoassay (ELISA) is highly specific and selective towards target molecules and is convenient for on-site detection. However, in many cases, lack of high sensitivity makes it hard to reveal a significant colorimetric signal for detecting a trace amount of target molecules. Thus, analytical instruments are required for detection, which limits the application of ELISA for on-site detection. In the present study, a highly sensitive and naked-eyed detectable colorimetric biosensor for chloramphenicol (CAP) was prepared by incorporating ELISA onto surfaces of microporous and nanofibrous membranes. The high specific surface areas of the nanofibers significantly increased the number of antibodies covalently linked onto the fiber surfaces and binding capacity of the sensor with antigens present in a sample. With such an integration, the sensitivity of the ELISA sensor was dramatically increased, and a trace number of targets could reveal a naked-eye detectable color. The immunoassay sensor exhibited a significant naked-eye distinguishable color to chloramphenicol (CAP) at 0.3 ng/ mL. The successful design and fabrication of the nanofibrous membrane immunoassay sensor provide new paths towards the development of on-site inspection sensors without the assistance from any instrument.

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Section: Fabrication Of Pva-co-pe Nanofibrous Membranesmentioning

confidence: 99%

Development of Improved Double-Nanobody Sandwich ELISAs for Human Soluble Epoxide Hydrolase Detection in Peripheral Blood Mononuclear Cells of Diabetic Patients and the Prefrontal Cortex of Multiple Sclerosis Patients

Morisseau

McReynolds

et al. 2020

Anal. Chem.

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“…Electrochemical immunosensors have enhanced sensitivity and offer a highly improved selectivity due to the specific antibody-antigen molecular recognition reaction. Thus, CAP detection using immunosensors was based either on the increase of the current generated by CAP reduction [ 104 ] or by the decrease of the signal of a redox probe, e.g., K 3 [Fe(CN) 6 ], [ 103 ] with increased concentrations of CAP that was bound to the CAP-antibody immobilized at the electrode surface. Aptasensors are electrodes modified with aptamers that are single stranded oligonucleotides with characteristic identification sites and configuration that enable a specific interaction with the analyte, thus enabling the selective accumulation of the target species.…”

Section: Principles Of Amphenicol Electrochemical Detectionmentioning

confidence: 99%

Past and Present of Electrochemical Sensors and Methods for Amphenicol Antibiotic Analysis

et al. 2022

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Amphenicols are broad-spectrum antibiotics. Despite their benefits, they also present toxic effects and therefore their presence in animal-derived food was regulated. Various analytical methods have been reported for their trace analysis in food and environmental samples, as well as in the quality control of pharmaceuticals. Among these methods, the electrochemical ones are simpler, more rapid and cost-effective. The working electrode is the core of any electroanalytical method because the selectivity and sensitivity of the determination depend on its surface activity. Therefore, this review offers a comprehensive overview of the electrochemical sensors and methods along with their performance characteristics for chloramphenicol, thiamphenicol and florfenicol detection, with a focus on those reported in the last five years. Electrode modification procedures and analytical applications of the recently described devices for amphenicol electroanalysis in various matrices (pharmaceuticals, environmental, foods), together with the sample preparation methods were discussed. Therefore, the information and the concepts contained in this review can be a starting point for future new findings in the field of amphenicol electrochemical detection.

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“…Another free-labeled immunosensor for chloramphenicol quantification was based on a screen-printed carbon electrode laminated with a layer of poly (vinyl alcohol- co -ethylene) (PVA- co -PE) nanofibrous membrane, that was covalently immobilized with an anti-chloramphenicol antibody [91]. The amperometrically monitored current of reduction of nitro groups of the captured antibiotic molecules on the antibody-modified screen-printed electrodes was an analytical signal.…”

Section: Recent Reports On Electrochemical Immunosensors Designatementioning

confidence: 99%

Electrochemical Immunosensors for Antibiotic Detection

2019

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Antibiotics are an important class of drugs destined for treatment of bacterial diseases. Misuses and overuses of antibiotics observed over the last decade have led to global problems of bacterial resistance against antibiotics (ABR). One of the crucial actions taken towards limiting the spread of antibiotics and controlling this dangerous phenomenon is the sensitive and accurate determination of antibiotics residues in body fluids, food products, and animals, as well as monitoring their presence in the environment. Immunosensors, a group of biosensors, can be considered an attractive tool because of their simplicity, rapid action, low-cost analysis, and especially, the unique selectivity arising from harnessing the antigen–antibody interaction that is the basis of immunosensor functioning. Herein, we present the recent achievements in the field of electrochemical immunosensors designed to determination of antibiotics.

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Ultrasensitive label-free electrochemical immunosensor based on PVA-co-PE nanofibrous membrane for the detection of chloramphenicol residues in milk

Cited by 85 publications

References 47 publications

Development of Improved Double-Nanobody Sandwich ELISAs for Human Soluble Epoxide Hydrolase Detection in Peripheral Blood Mononuclear Cells of Diabetic Patients and the Prefrontal Cortex of Multiple Sclerosis Patients

Development of Improved Double-Nanobody Sandwich ELISAs for Human Soluble Epoxide Hydrolase Detection in Peripheral Blood Mononuclear Cells of Diabetic Patients and the Prefrontal Cortex of Multiple Sclerosis Patients

Past and Present of Electrochemical Sensors and Methods for Amphenicol Antibiotic Analysis

Electrochemical Immunosensors for Antibiotic Detection

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