Ultrasensitive, Selective, and Highly Stable Bioelectronic Nose That Detects the Liquid and Gaseous Cadaverine

Abstract

Field-effect transistor (FET) devices based on conductive nanomaterials have been used to develop biosensors. However, development of FET-based biosensors that allow efficient stability, especially in the gas phase, for obtaining reliable and reproducible responses remains a challenge. In this study, we developed a nanodisc (ND)-functionalized bioelectronic nose (NBN) based on a nickel (Ni)-decorated carboxylated polypyrrole nanoparticle (cPPyNP)-FET that offers the detection of liquid and gaseous cadaverine (CV). The TAAR13c, specifically binding to CV, which is an indicator of food spoilage, was successfully constructed in NDs. The NBN was fabricated by the oriented assembly of TAAR13c-embedded NDs (T13NDs) onto the transistor with Ni/cPPyNPs. The NBN showed high performance in selectivity and sensitivity for the detection of CV, with excellent stability in both aqueous and gas phases. Moreover, the NBN allowed efficient measurement of corrupted real-food samples. It demonstrates the ND-based device can allow the practical biosensor that provides high stability in the gas phase.

Bioelectronic sensor mimicking the human neuroendocrine system for the detection of hypothalamic-pituitary-adrenal axis hormones in human blood

“…In this method, the receptor and lipid bilayer are wrapped with membrane scaffold proteins (MSPs). The stability of NDs embedded with GPCRs and the superiority in detection by the sensor is reported (Oh et al 2019). However, studies describing the reconstitutions of CRHR1 and MC2R using NDs or the application of the CRHR1ND and MC2RND in the sensor have not been carried out before.…”

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

“…Proteins interacting with volatile organic compounds (VOCs) in the human nose (a) and examples of olfactory sensors designed from biosourced materials (b). Trace amine associated receptors (TAAR) in lipidic nanodiscs grafted on a field-effect transistor for the detection of cadaverine (adapted from [15]). (c) Three different kind of odorant binding proteins (OBPs) deposited on surface acoustic wave sensors for carvone sensing (adapted from [16]).…”

“…First, their structure is essential for their binding function, and a lipidic environment is necessary to preserve it. Membrane fractions [20,21], then nanovesicles or nanoliposomes [22][23][24][25], and eventually nanodiscs ( Figure 2b) [15,26,27] have been used to achieve their immobilization on sensors. Second, the fact that they are not soluble makes their production challenging.…”

Market Perspectives and Future Fields of Application of Odor Detection Biosensors—A Systematic Analysis

“…No high repeat accuracy can be guaranteed yet. 0 [32,35,36] High selectivity It can be tested very specifically for certain substances. As illustrated in Figure 2, biosensors have advantages in terms of sensitivity, selectivity, specificity, accuracy, and resolution.…”