“…Compared with other methodology in tissue thickness measurement, like using optical [26] and needle probe [4], ultrasound-based techniques have been proved to be most suitable for in vivo tissue thickness measurement because of its noninvasiveness. Ultrasound indentation is easy to use for in vivo stiffness measurement, and it has been widely used for assessment of residual limb tissues, diabetic foot tissue, fibrotic neck tissue with radiotherapy, and spinal tissues [25][26][27][28][29][30]. It has also been demonstrated that the proposed ultrasound indentation technique can be used to accurately identify the abnormality of articular cartilage [21,31].…”