Ultrasound microbubbles enhance human β-defensin 3 against biofilms

Li, Shoumin; Zhu, Chen; Fang, Shiyuan; Zhang, Weiwei; He, Na; Xu, Wei; Kong, Rong; Shang, Xifu

doi:10.1016/j.jss.2015.05.030

Cited by 27 publications

(32 citation statements)

References 50 publications

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“…In contrast, biofilms in the group treated with the UTS were less dense, with disruption of cell clumps and higher proportion of dead cells. This observation is in agreement with an in vivo study that investigated the effectiveness of combining ultrasound‐targeted microbubble destruction (UTMD) and human β‐defensin 3 (HBD‐3) against antibiotic‐resistant Staphylococcus strains, in which CLSM showed the group treated with HBD‐3 and UTMD to have significantly less (<30%) live bacteria compared with the control (100%) group . Another study involving application of 500‐kHz ultrasound resulted in inactivation of E. coli and S. mutans .…”

Section: Discussionsupporting

confidence: 85%

“…This observation is in agreement with an in vivo study that investigated the effectiveness of combining ultrasound-targeted microbubble destruction (UTMD) and human β-defensin 3 (HBD-3) against antibiotic-resistant Staphylococcus strains, in which CLSM showed the group treated with HBD-3 and UTMD to have significantly less (<30%) live bacteria compared with the control (100%) group. 26 Another study involving application of 500-kHz ultrasound resulted in inactivation of E. coli and S. mutans. 27 However, with these ultrasound treatment studies, CLSM imaging showed some retention of structural organization of the biofilm, unlike our present study that resulted in total disruption of the structural organization of the biofilm.…”

Section: Discussionmentioning

confidence: 99%

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In vitro evaluation of the effects of Ultrasound Tongue Scraper on bacteria and biofilm formation

Azees

Menon

Kasundra

2019

J of Invest & Clin Dent

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Aim Oral malodor is a common condition caused by some Gram‐negative oral bacteria, among which are the 3 red complex bacteria (RCB). The present study investigated the effectiveness of the Ultrasound Tongue Scraper (UTS) to disrupt the structural morphology of the bacteria and their biofilm. Methods While developing over 72 hours, multispecies biofilms of RCB (Porphromonas gingivalis, Tryponema denticola, Tannerella forsythia) were treated every 24 hours with 1.6‐MHz ultrasound waves generated with UTS. An untreated group served as controls. Confocal laser scanning microscopy was used to determine the biofilm thickness, biomass and live : dead cell ratio at each time point (24, 48 and 72 hours). Biofilm morphology and bacteria ultrastructure were viewed using scanning/transmission electron microscopy, respectively. Data were analyzed using ANOVA and Tukey tests. Results At each time point, the 3 variables were significantly lower in treated samples than the untreated. Significant biofilm disruption was observed in treated samples at each time period while the untreated had intact biofilm morphology. Cells in treated samples showed disrupted cell wall, cytoplasmic material, huge vacuoles and heterogeneity in electron density, while these cell organelles remained intact in untreated samples. Conclusion The UTS has an inhibitory effect on RCB and could be useful for oral malodor management.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of the effects of Ultrasound Tongue Scraper on bacteria and biofilm formation

Azees

Menon

Kasundra

2019

J of Invest & Clin Dent

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“…Only one study used mixed types of bacteria (Agarwal et al 2014). Two articles compared two different types of bacteria (Zhu et al 2013;Li et al 2015), and another compared a bacterium and a fungus (Tandiono et al 2012). Only four studies used patient-derived clinical isolates originating from a central venous catheter (Hu et al 2018b), infective endocarditis blood culture (Lattwein et al 2018), pneumonia-induced sputum (Fu et al 2019) and urine from a patient with lower urinary tract symptoms (Horsley et al 2019).…”

Section: Bacteriamentioning

confidence: 99%

“…In addition to the antibiotics, three other antimicrobials were investigated: sodium hypochlorite (NaOCl), lysozyme and human b-defensin 3 ( Table 1). The studies included either clinically appropriate antimicrobials (dental, NaOCl [Halford et al 2012]) or antimicrobials testing a new approach (lysozyme [Liao et al 2017]; human b-defensin 3, [Zhu et al 2013, Li et al 2015, Zhou et al 2018). Sodium hypochlorite is a disinfectant used widely in endodontic irrigation and health care facilities (Estrela et al 2002).…”

Section: Experimental Approachesmentioning

confidence: 99%

“…Thirty percent of the articles on sonobactericide (8/27) have investigated therapeutic efficacy in pre-clinical animal models (Table 1). Four groups chose to emulate implanted medical device infections using subcutaneous implants, near the spine, with biofilm grown on catheter pieces or polyethylene disks in rabbits (He et al 2011, Dong et al 2018) and titanium plates in mice (Li et al 2015;Zhou et al 2018). Microbubbles were injected subcutaneously into the implant area before ultrasound.…”

Section: Experimental Approachesmentioning

confidence: 99%

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Sonobactericide: An Emerging Treatment Strategy for Bacterial Infections

Lattwein

Shekhar

Kouijzer

et al. 2020

Ultrasound in Medicine & Biology

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Ultrasound has been developed as both a diagnostic tool and a potent promoter of beneficial bioeffects for the treatment of chronic bacterial infections. Bacterial infections, especially those involving biofilm on implants, indwelling catheters and heart valves, affect millions of people each year, and many deaths occur as a consequence. Exposure of microbubbles or droplets to ultrasound can directly affect bacteria and enhance the efficacy of antibiotics or other therapeutics, which we have termed sonobactericide. This review summarizes investigations that have provided evidence for ultrasound-activated microbubble or droplet treatment of bacteria and biofilm. In particular, we review the types of bacteria and therapeutics used for treatment and the in vitro and pre-clinical experimental setups employed in sonobactericide research. Mechanisms for ultrasound enhancement of sonobactericide, with a special emphasis on acoustic cavitation and radiation force, are reviewed, and the potential for clinical translation is discussed. (

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Different Aspects of Using Ultrasound to Combat Microorganisms

Nakonechny

Nisnevitch

2021

Adv Funct Materials

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Growing resistance of microorganisms to antibiotics due to their widespread use has led to multiple bacterial infections posing a serious threat to health and human life. Low-frequency ultrasound is one of physical methods for inactivation of pathogenic microbial cells. Application of ultrasound is safe, demonstrates good tissue penetration without significant attenuation of energy, and does not induce microbial resistance. Bactericidal effect of ultrasound is based on acoustic cavitation-the growth and collapse of microbubbles in a liquid medium, resulting in shock waves, shear forces, and microjets which cause irreversible damage and inactivation of microorganisms. The present review combines and analyzes literature data on in vitro and in vivo studies and summarizes works demonstrating the ability of ultrasound, alone or in combination with other methods, to combat pathogenic microorganisms. The results of various studies presented in this review show that low-frequency ultrasound has a noticeable antimicrobial effect on planktonic cells of microorganisms and biofilms. Ultrasound synergistically enhances the effectiveness of other antibacterial agents and activates molecules called sonosensitizers, resulting in the formation of compounds toxic to microbial cells. Ultrasound can also promote local release of antimicrobial drugs from liposomes, as well as from medical implants.

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Ultrasound microbubbles enhance human β-defensin 3 against biofilms

Cited by 27 publications

References 50 publications

In vitro evaluation of the effects of Ultrasound Tongue Scraper on bacteria and biofilm formation

In vitro evaluation of the effects of Ultrasound Tongue Scraper on bacteria and biofilm formation

Sonobactericide: An Emerging Treatment Strategy for Bacterial Infections

Different Aspects of Using Ultrasound to Combat Microorganisms

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