Ultrasound Microbubbles Enhance the Activity of Vancomycin Against <i>Staphylococcus epidermidis</i> Biofilms In Vivo

Dong, Ying; Li, Junshuai; Li, Pan; Yu, Jialin

doi:10.1002/jum.14475

Cited by 32 publications

(34 citation statements)

References 35 publications

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“…Thirty percent of the articles on sonobactericide (8/27) have investigated therapeutic efficacy in pre-clinical animal models (Table 1). Four groups chose to emulate implanted medical device infections using subcutaneous implants, near the spine, with biofilm grown on catheter pieces or polyethylene disks in rabbits (He et al 2011, Dong et al 2018) and titanium plates in mice (Li et al 2015;Zhou et al 2018). Microbubbles were injected subcutaneously into the implant area before ultrasound.…”

Section: Experimental Approachesmentioning

confidence: 99%

“…For each of the three ultrasound exposures (20 min) per day, He et al (2011) injected 200 mL of microbubbles (2 £ 10 8 to 5 £ 10 8 /mL) every 5 min, and both Li et al (2015) and Zhou et al (2018) injected 30 mL once, 2 £ 10 8 to 5 £ 10 8 /mL and concentration not disclosed, respectively. Dong et al (2018) applied ultrasound twice a day for 5 min, and injected 500 ml (1.2 £ 10 9 /mL diluted to 1% v/v) each time. All studies used a 50% duty cycle.…”

Section: Experimental Approachesmentioning

confidence: 99%

“…Scanning electron microscopy ( Fig. 5) was also used to complement confocal findings 2013, Zhu et al 2013Li et al 2015;Guo et al 2017;Dong et al 2018;Zhou et al 2018). For transmission microscopy, sample preparation of biofilms required removal from culture plate; thus, the biofilms were mechanically scraped, which could alter cellular structure (Hu et al, 2018b).…”

Section: Experimental Approachesmentioning

confidence: 99%

“…Four groups produced custom microbubbles for coadministration with an antibiotic (Ikeda-Dantsuji et al 2011;Dong et al 2013Dong et al , 2017Dong et al , 2018, an antibiotic encapsulated in a liposome (Fu et al 2019) or stem cells (Yi et al 2016). Dong et al (2013Dong et al ( , 2017Dong et al ( , 2018 produced lipid-coated microbubbles with a gas core of C 3 F 8 and diameter of 4À6 mm. The coating consisted of the lipids DSPC and 1,2-dipalmitoyl-sn-glycerol-3-phosphate-ethanolamine at a molar ratio of 66:34.…”

Section: Cavitation Nuclei For Sonobactericidementioning

confidence: 99%

“…Another important consideration for microbubble oscillation is the viscosity of the surrounding medium and confinement because oscillations are damped when the viscosity increases or when microbubbles are confined (Kooiman et al 2014). For the in vivo studies, the microbubbles were confined in tissue as a result of injection into the area of the implanted catheter (Dong et al 2018), dish (He et al 2011), titanium plate (Li et al 2015) or tibial canal (Lin et al 2015). It appears that these studies did not consider the effect of attenuation of ultrasound by overlying tissue.…”

Section: Cavitation Nuclei For Sonobactericidementioning

confidence: 99%

See 4 more Smart Citations

Sonobactericide: An Emerging Treatment Strategy for Bacterial Infections

Lattwein

Shekhar

Kouijzer

et al. 2020

Ultrasound in Medicine & Biology

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Ultrasound has been developed as both a diagnostic tool and a potent promoter of beneficial bioeffects for the treatment of chronic bacterial infections. Bacterial infections, especially those involving biofilm on implants, indwelling catheters and heart valves, affect millions of people each year, and many deaths occur as a consequence. Exposure of microbubbles or droplets to ultrasound can directly affect bacteria and enhance the efficacy of antibiotics or other therapeutics, which we have termed sonobactericide. This review summarizes investigations that have provided evidence for ultrasound-activated microbubble or droplet treatment of bacteria and biofilm. In particular, we review the types of bacteria and therapeutics used for treatment and the in vitro and pre-clinical experimental setups employed in sonobactericide research. Mechanisms for ultrasound enhancement of sonobactericide, with a special emphasis on acoustic cavitation and radiation force, are reviewed, and the potential for clinical translation is discussed. (

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Section: Experimental Approachesmentioning

confidence: 99%

Section: Experimental Approachesmentioning

confidence: 99%

Section: Experimental Approachesmentioning

confidence: 99%

Section: Cavitation Nuclei For Sonobactericidementioning

confidence: 99%

Section: Cavitation Nuclei For Sonobactericidementioning

confidence: 99%

See 3 more Smart Citations

Sonobactericide: An Emerging Treatment Strategy for Bacterial Infections

Lattwein

Shekhar

Kouijzer

et al. 2020

Ultrasound in Medicine & Biology

View full text Add to dashboard Cite

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Diagnosis of Physical Stimuli Response Enhances the Anti‐Quorum Sensing Agents in Controlling Bacterial Biofilm Formation

Ramachandran,

Palanisamy,

Chackaravarthy

et al. 2024

Nanoscience and Nanotechnology for Smart Prevention, Diagnostics and Therapeutics

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Different Aspects of Using Ultrasound to Combat Microorganisms

Nakonechny

Nisnevitch

2021

Adv Funct Materials

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Growing resistance of microorganisms to antibiotics due to their widespread use has led to multiple bacterial infections posing a serious threat to health and human life. Low-frequency ultrasound is one of physical methods for inactivation of pathogenic microbial cells. Application of ultrasound is safe, demonstrates good tissue penetration without significant attenuation of energy, and does not induce microbial resistance. Bactericidal effect of ultrasound is based on acoustic cavitation-the growth and collapse of microbubbles in a liquid medium, resulting in shock waves, shear forces, and microjets which cause irreversible damage and inactivation of microorganisms. The present review combines and analyzes literature data on in vitro and in vivo studies and summarizes works demonstrating the ability of ultrasound, alone or in combination with other methods, to combat pathogenic microorganisms. The results of various studies presented in this review show that low-frequency ultrasound has a noticeable antimicrobial effect on planktonic cells of microorganisms and biofilms. Ultrasound synergistically enhances the effectiveness of other antibacterial agents and activates molecules called sonosensitizers, resulting in the formation of compounds toxic to microbial cells. Ultrasound can also promote local release of antimicrobial drugs from liposomes, as well as from medical implants.

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Ultrasound Microbubbles Enhance the Activity of Vancomycin Against Staphylococcus epidermidis Biofilms In Vivo

Abstract: Ultrasound microbubbles enhanced the antibacterial effect of vancomycin against S epidermidis biofilms in vivo without exerting obvious harms to the animals.

Cited by 32 publications

References 35 publications

Sonobactericide: An Emerging Treatment Strategy for Bacterial Infections

Sonobactericide: An Emerging Treatment Strategy for Bacterial Infections

Diagnosis of Physical Stimuli Response Enhances the Anti‐Quorum Sensing Agents in Controlling Bacterial Biofilm Formation

Different Aspects of Using Ultrasound to Combat Microorganisms

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