Ultrastructural analysis of the low level laser therapy effects on the lesioned anterior tibial muscle in the Gerbil

Iyomasa, Daniela Mizusaki; Garavelo, Ivania; Iyomasa, Mamie Mizusaki; Watanabe, Ii-sei; Issa, João Paulo Mardegan

doi:10.1016/j.micron.2009.02.002

Cited by 43 publications

(27 citation statements)

References 35 publications

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“…The energy density established was 4 J/cm² per point as indicated for an acute inflammatory process 28 . As in this work, the effectiveness of laser therapy (633 nm) applied at a low dose was verified by Lyomasa et al 29 , who used a HeNe laser at 5 J/cm² and 10 J/cm² on young gerbils after TA muscle injury. After seven consecutive days of treatment, increased mitochondrial activity in muscle fibers, an increase of fibroblasts and macrophages and the stimulation of angiogenesis were noted, suggesting the efficacy of both doses in muscle regeneration.…”

Section: Discussionsupporting

confidence: 70%

Evaluation of muscle regeneration in aged animals after treatment with low-level laser therapy

Pertille¹,

Macedo²,

Oliveira³

2012

Rev. bras. fisioter.

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Section: Discussionsupporting

confidence: 70%

Evaluation of muscle regeneration in aged animals after treatment with low-level laser therapy

Pertille¹,

Macedo²,

Oliveira³

2012

Rev. bras. fisioter.

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“…The consequence is a substantial rise in ATP production that leads to the reactivation of DNA, RNA and proteins synthesis, increasing cell proliferation. [17][18][19] The activation of satellite cells is regulated by growth factors such as transforming growth factor ðTGFÞ-β, basic fibroblast growth factor (FGFb), and insulin-like growth factor (IGF-1). The recruitment of immune cells, triggered by inflammatory mediators, is a necessary response for the phagocytosis of cellular debris.…”

Section: Introductionmentioning

confidence: 99%

Low-level laser therapy on skeletal muscle inflammation: evaluation of irradiation parameters

2014

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We evaluated the effect of different irradiation parameters in low-level laser therapy (LLLT) for treating inflammation induced in the gastrocnemius muscle of rats through cytokines concentration in systemic blood and analysis of muscle tissue. We used continuous (830 and 980 nm) and pulsed illuminations (830 nm). Animals were divided into five groups per wavelength (10, 20, 30, 40, and 50 mW), and a control group. LLLT was applied during 5 days with a constant irradiation time and area. TNF-α, IL-1β, IL-2, and IL-6 cytokines were quantified by ELISA. Inflammatory cells were counted using microscopy. Identical methodology was used with pulsed illumination. Average power (40 mW) and duty cycle were kept constant (80%) at five frequencies (5, 25, 50, 100, and 200 Hz). For continuous irradiation, treatment effects occurred for all doses, with a reduction of TNF-α, IL-1β, and IL-6 cytokines and inflammatory cells. Continuous irradiation at 830 nm was more effective, a result explained by the action spectrum of cytochrome c oxidase (CCO). Best results were obtained for 40 mW, with data suggesting a biphasic dose response. Pulsed wave irradiation was only effective for higher frequencies, a result that might be related to the rate constants of the CCO internal electron transfer process.

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“…Positive results have been reported with LLLT in several experimental models of skeletal muscle injury and repair [19–22]. This therapeutic modality was able to improve muscle repair by inducing a rapid growth of capillary vessels in the injured area [23, 24], a remarkable regeneration of muscle fibers [25–27] and parallel orientation of regenerating myofibers [28]. …”

Section: Introductionmentioning

confidence: 99%

Low-level laser therapy (808 nm) contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion

et al. 2012

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Muscle regeneration is a complex phenomenon, involving replacement of damaged fibers by new muscle fibers. During this process, there is a tendency to form scar tissue or fibrosis by deposition of collagen that could be detrimental to muscle function. New therapies that could regulate fibrosis and favor muscle regeneration would be important for physical therapy. Low-level laser therapy (LLLT) has been studied for clinical treatment of skeletal muscle injuries and disorders, even though the molecular and cellular mechanisms have not yet been clarified. The aim of this study was to evaluate the effects of LLLT on molecular markers involved in muscle fibrosis and regeneration after cryolesion of the tibialis anterior (TA) muscle in rats. Sixty Wistar rats were randomly divided into three groups: control, injured TA muscle without LLLT, injured TA muscle treated with LLLT. The injured region was irradiated daily for four consecutive days, starting immediately after the lesion using an AlGaAs laser (808 nm, 30 mW, 180 J/cm2; 3.8 W/cm2, 1.4 J). The animals were sacrificed on the fourth day after injury. LLLT significantly reduced the lesion percentage area in the injured muscle (p<0.05), increased mRNA levels of the transcription factors MyoD and myogenin (p<0.01) and the pro-angiogenic vascular endothelial growth factor (p<0.01). Moreover, LLLT decreased the expression of the profibrotic transforming growth factor TGF-β mRNA (p<0.01) and reduced type I collagen deposition (p<0.01). These results suggest that LLLT could be an effective therapeutic approach for promoting skeletal muscle regeneration while preventing tissue fibrosis after muscle injury.

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Ultrastructural analysis of the low level laser therapy effects on the lesioned anterior tibial muscle in the Gerbil

Cited by 43 publications

References 35 publications

Evaluation of muscle regeneration in aged animals after treatment with low-level laser therapy

Evaluation of muscle regeneration in aged animals after treatment with low-level laser therapy

Low-level laser therapy on skeletal muscle inflammation: evaluation of irradiation parameters

Low-level laser therapy (808 nm) contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion

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