Ultrastructural characteristics of the external surfaces of <i>Pasteurella pneumotropica</i> from mice and <i>Pasteurella multocida</i> from rabbits

Kawamoto, Eiichi; Okiyama, Emi; Sasaki, Hiraku; Sawada, Takuo; Mikazuki, Katsumi; Ueshiba, Hidehiro

doi:10.1258/002367707780378087

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…[P . ] pneumotropica possess a slight extracellular fibrous material associated with the cell wall when stained with ruthenium red [ 53 ]. One can speculate that the treatment of [P . ]…”

Section: Discussionmentioning

confidence: 99%

Characterization of Biofilm Formation in [Pasteurella] pneumotropica and [Actinobacillus] muris Isolates of Mouse Origin

et al. 2015

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[Pasteurella] pneumotropica biotypes Jawetz and Heyl and [Actinobacillus] muris are the most prevalent Pasteurellaceae species isolated from laboratory mouse. However, mechanisms contributing to their high prevalence such as the ability to form biofilms have not been studied yet. In the present investigation we analyze if these bacterial species can produce biofilms in vitro and investigate whether proteins, extracellular DNA and polysaccharides are involved in the biofilm formation and structure by inhibition and dispersal assays using proteinase K, DNase I and sodium periodate. Finally, the capacity of the biofilms to confer resistance to antibiotics is examined. We demonstrate that both [P.] pneumotropica biotypes but not [A.] muris are able to form robust biofilms in vitro, a phenotype which is widely spread among the field isolates. The biofilm inhibition and dispersal assays by proteinase and DNase lead to a strong inhibition in biofilm formation when added at the initiation of the biofilm formation and dispersed pre-formed [P.] pneumotropica biofilms, revealing thus that proteins and extracellular DNA are essential in biofilm formation and structure. Sodium periodate inhibited the bacterial growth when added at the beginning of the biofilm formation assay, making difficult the assessment of the role of β-1,6-linked polysaccharides in the biofilm formation, and had a biofilm stimulating effect when added on pre-established mature biofilms of [P.] pneumotropica biotype Heyl and a majority of [P.] pneumotropica biotype Jawetz strains, suggesting that the presence of β-1,6-linked polysaccharides on the bacterial surface might attenuate the biofilm production. Conversely, no effect or a decrease in the biofilm quantity was observed by biofilm dispersal using sodium periodate on further biotype Jawetz isolates, suggesting that polysaccharides might be incorporated in the biofilm structure. We additionally show that [P.] pneumotropica cells enclosed in biofilms were less sensitive to treatment with amoxicillin and enrofloxacin than planktonic bacteria. Taken together, these findings provide a first step in understanding of the biofilm mechanisms in [P.] pneumotropica, which might contribute to elucidation of colonization and pathogenesis mechanisms for these obligate inhabitants of the mouse mucosa.

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“…[P . ] pneumotropica possess a slight extracellular fibrous material associated with the cell wall when stained with ruthenium red [ 53 ]. One can speculate that the treatment of [P . ]…”

Section: Discussionmentioning

confidence: 99%

Characterization of Biofilm Formation in [Pasteurella] pneumotropica and [Actinobacillus] muris Isolates of Mouse Origin

et al. 2015

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“…However, Boot et al [3] showed thin fimbriae by using negative staining and electron microscopy. Using transmission electron microscopy, we recently revealed the outer structure of the cell wall of P. pneumotropica [11]. Based on its biochemical characteristics, P. pneumotropica is classified as biovars Jawetz or Heyl.…”

Section: Discussionmentioning

confidence: 99%

“…The strain possessed the outermost surface materials on the bacterial cells [11] and was identified as a-1 by amplified 16S ribosomal DNA restriction analysis [20]. The strain was thawed from frozen stock (-80°C) and grown overnight on 5% horse blood agar plates (Climedia, Sankyo Junyaku Co., Ltd., Tokyo, Japan) and checked for purity.…”

Section: Preparation Of P Pneumotropica Inoculummentioning

confidence: 99%

Pathogenicity of Pasteurella pneumotropica in Immunodeficient NOD/ShiJic-scid/Jcl and Immunocompetent Crlj:CD1 (ICR) Mice

et al. 2011

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Abstract:Pasteurella pneumotropica is an opportunistic pathogen in rodents. Natural infection in immunodeficient animals suggests that immunodeficiency is a major factor in P. pneumotropica pathogenesis. To understand this process, we performed clinical, pathological and bacteriological studies of immunodeficient NOD/ShiJic-scid/Jcl and immunocompetent Crlj:CD1 (ICR) mice experimentally infected with P. pneumotropica ATCC 35149. From 14 days postinoculation, some of P. pneumotropica-infected NOD/ShiJic-scid/Jcl mice developed clinical signs of weight loss. Three of 10 P. pneumotropica-infected NOD/ShiJic-scid/Jcl mice developed clinical signs of depression, ruffled coat, and weight loss and died at 27, 34, and 59 days postinoculation. At 35 days postinoculation, almost all P. pneumotropica-infected NOD/ShiJic-scid/Jcl mice had lung abscesses. The bacteria were isolated from the upper and lower respiratory tracts, including the lungs, and blood. In contrast, P. pneumotropicainfected ICR mice exhibited no clinical signs or lesions. The bacteria were isolated from the upper, but not the lower respiratory tracts. We developed an animal model for understanding host interactions with P. pneumotropica.

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“…Capsule was visualized via transmission electron microscopy (TEM) using previously described methods (21)(22)(23)…”

Section: Transmission Electron Microscopy (Tem) For Capsule Visualizamentioning

confidence: 99%

Generation and Evaluation of a Glaesserella (Haemophilus) parasuis Capsular Mutant

et al. 2020

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Glaesserella (Haemophilus) parasuis is a commensal bacterium of the upper respiratory tract in pigs and also the causative agent of Glässer’s disease, which causes significant morbidity and mortality in pigs worldwide. Isolates are characterized into 15 serovars by their capsular polysaccharide, which has shown a correlation with isolate pathogenicity. To investigate the role the capsule plays in G. parasuis virulence and host interaction, a capsule mutant of the serovar 5 strain HS069 was generated (HS069Δcap) through allelic exchange following natural transformation. HS069Δcap was unable to cause signs of systemic disease during a pig challenge study and had increased sensitivity to complement killing and phagocytosis by alveolar macrophages. Compared with the parent strain, HS069Δcap produced more robust biofilm and adhered equivalently to 3D4/31 cells; however, it was unable to persistently colonize the nasal cavity of inoculated pigs, with all pigs clearing HS069Δcap by 5 days postchallenge. Our results indicate the importance of the capsular polysaccharide to G. parasuis virulence as well as nasal colonization in pigs.

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Ultrastructural characteristics of the external surfaces of Pasteurella pneumotropica from mice and Pasteurella multocida from rabbits

Cited by 7 publications

References 22 publications

Characterization of Biofilm Formation in [Pasteurella] pneumotropica and [Actinobacillus] muris Isolates of Mouse Origin

Characterization of Biofilm Formation in [Pasteurella] pneumotropica and [Actinobacillus] muris Isolates of Mouse Origin

Pathogenicity of Pasteurella pneumotropica in Immunodeficient NOD/ShiJic-scid/Jcl and Immunocompetent Crlj:CD1 (ICR) Mice

Generation and Evaluation of a Glaesserella (Haemophilus) parasuis Capsular Mutant

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