Ultrastructural effects of two phthalocyanines in CHO-K1 and HeLa cells after laser irradiation

Pazos, Marcelo de Castro; Pacheco‐Soares, Cristina; Silva, Newton Soares da; DaMatta, Renato Augusto; Pacheco, Marcos Tadeu Tavares

doi:10.32604/biocell.2003.27.301

Cited by 15 publications

(4 citation statements)

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“…This shows that the cellular uptake of ALA depends on the type of cells. Also for a photosensitizer other than ALA we need different incubation time for HeLa cells [16] showing that the cellular uptake of a photosensitizer might be structure dependent.…”

Section: Resultsmentioning

confidence: 99%

In vitrostudy of 5-aminolevulinic acid-based photodynamic therapy for apoptosis in human cervical HeLa cell line

Atif¹,

Firdous²,

Khurshid³

et al. 2009

Laser Phys. Lett.

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5-aminolevulanic acid (ALA), belonging among the promising second generation of sensitizers, was evaluated as an inducer of photodamage on HeLa (human cervical adenocarcinoma) cell line. A diode laser (635 nm) was used as a source for initiation of the photodynamic effect. We studied the influence of different incubation times, various concentrations of sensitizer, different irradiation doses and various combinations of sensitizer and light doses on the photodamage of HeLa cells. Viability of cells was determined by means of neutral red assay. The quantitative cellular uptake of ALA sensitizer was done by spectrophotometric measurements. No prominent cytotoxic or phototoxic effects on HeLa were observed due to sensitizer or light doses when studied independently of each other. However phototoxicity evoked by laser irradiated sensitizer was detected in HeLa cell line.

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Section: Resultsmentioning

confidence: 99%

In vitrostudy of 5-aminolevulinic acid-based photodynamic therapy for apoptosis in human cervical HeLa cell line

Atif¹,

Firdous²,

Khurshid³

et al. 2009

Laser Phys. Lett.

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“…The predominant mechanism of cell death is determined by the subcellular localization of ROS generation and subsequent cellular targets of ROS [ 3 , 51 ]. Among these subcellular targets are most often the endoplasmic reticulum, mitochondria, lysosomes and the plasma membrane [ 4 , 52 , 53 , 54 ]. Molecular targets of ROS are mainly the thiol groups of proteins, membrane lipids and DNA [ 4 , 55 ].…”

Section: Effect On Cellular Levelmentioning

confidence: 99%

Advances in Liposome-Encapsulated Phthalocyanines for Photodynamic Therapy

Rak¹,

Kabešová

Beneš

et al. 2023

Life

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This updated review aims to describe the current status in the development of liposome-based systems for the targeted delivery of phthalocyanines for photodynamic therapy (PDT). Although a number of other drug delivery systems (DDS) can be found in the literature and have been studied for phthalocyanines or similar photosensitizers (PSs), liposomes are by far the closest to clinical practice. PDT itself finds application not only in the selective destruction of tumour tissues or the treatment of microbial infections, but above all in aesthetic medicine. From the point of view of administration, some PSs can advantageously be delivered through the skin, but for phthalocyanines, systemic administration is more suitable. However, systemic administration places higher demands on advanced DDS, active tissue targeting and reduction of side effects. This review focuses on the already described liposomal DDS for phthalocyanines, but also describes examples of DDS used for structurally related PSs, which can be assumed to be applicable to phthalocyanines as well.

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“…Finally, cells were washed again and dehydrated in acetone and embedded in Epon. After sectioning, cells were contrasted with uranyl acetate for 30 minutes and lead citrate for five min [15]. The stained grids were examined and photographed with transmission electron microscope at 60 kV.…”

Section: Electron Microscopic Studiesmentioning

confidence: 99%

The chemosensitizing effect of aqueous extract of sweet fennel on cisplatin treated HeLa cells

Ramadan¹,

Sait²,

Anfinan³

et al. 2016

Clin. Exp. Obstet. Gynecol.

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Background: Cisplatin is an important chemotherapeutic agent that is widely used in treatment of several malignancies, but its side effects on normal tissues and organs limit its use. The aim of this study was to evaluate the effect of aqueous extract of sweet fennel alone and in combination with cisplatin on human cervical cancer adenocarcinoma cell line (HeLa cells) searching for an effective, inexpensive therapy with minimal side effects. Materials and Methods: HeLa cell line was used to study the cytotoxic effect of different concentrations of the aqueous extract of sweet fennel alone and in combination with 50 μg/ml cisplatin. Quantitative measure of drug interaction was quantified by the combination index. Gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) were used to analyze the sweet fennel decoction. MTT assay was used to examine cell viability percentage. Electron microscopy was applied to study the ultrastructure of the cells. Results: The phenyl propanoids (23%) and phenols (12%) constituted the highest percentage of the aqueous extract. Increasing the concentration of sweet fennel from 50 μg/ml to 80 μg/ml, decreased the percentage of the cell viability of HeLa cells from 86.74% to 78.28%, respectively. Further decrease to 11.31% was demonstrated when 50 μg/ml of fennel was combined with 50 μg/ml cisplatin (additive effect). In addition to the signs of apoptosis observed in HeLa cells at 50 μg/ml of fennel, disruption of both nuclear and cytoplasmic membranes and presence of autophagolysosomes were noticed at a dose of 80 μg/ml. Combination of 50 μg/ml of cisplatin with 60, 70, and 80 μg/ml of sweet fennel revealed no significant difference in comparison to cisplatin alone. The combination with 50 μg/ml of sweet fennel revealed marked vacuolization of the cytoplasm, fragmentation of the nucleus, and complete disruption of nuclear membrane. Conclusion: Combination of cisplatin and the 50 μg/ml of the fennel could enhance cervical cancer growth inhibition. This combination could be effective in lowering the dose of single or repeated cumulative courses of cisplatin and hence decreases its hazardous side effects. In vivo studies and the evaluation of different combination doses of cisplatin and sweet fennel are recommended.

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Ultrastructural effects of two phthalocyanines in CHO-K1 and HeLa cells after laser irradiation

Cited by 15 publications

References 0 publications

In vitrostudy of 5-aminolevulinic acid-based photodynamic therapy for apoptosis in human cervical HeLa cell line

In vitrostudy of 5-aminolevulinic acid-based photodynamic therapy for apoptosis in human cervical HeLa cell line

Advances in Liposome-Encapsulated Phthalocyanines for Photodynamic Therapy

The chemosensitizing effect of aqueous extract of sweet fennel on cisplatin treated HeLa cells

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