<i>In vitro</i>study of 5-aminolevulinic acid-based photodynamic therapy for apoptosis in human cervical HeLa cell line

Atif, M.; Firdous, S.; Khurshid, Asma; Noreen, L.; Zaidi, S. S. Z.; Ikram, Manzoor

doi:10.1002/lapl.200910087

Cited by 68 publications

(53 citation statements)

References 21 publications

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“…1 and Fig. 2, which is in excellent agreement with the previous reported study [12]. It is clear from the Fig 2. that the behavior of the different concentration of ALA is approximately same.…”

Section: Resultssupporting

confidence: 94%

“…After incubation, cellular absorption of ALA was quantified by measuring the optical density using microwell plate reader at a wavelength of 405 nm corresponding to the absorption wavelength of the Aminolevulonic acid in cells and the cytotoxicity was evaluated by means of neutral red assay as described in our previous work [12].…”

Section: Ala Cytotoxicity and Phototoxicity Testsmentioning

confidence: 99%

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Study of the efficacy of 5-ALA mediated photodynamic therapy on human rhabdomyosarcoma cell line (RD)

Fakhar-e-Alam¹,

Firdous²,

Zaidi³

et al. 2010

Laser Phys. Lett.

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The aim of this study was to investigate the mechanism of cell death by photodynamic therapy (PDT) in the Rhabdomyosarcoma (RD) cell line. The present study evaluates the effects of photodynamic therapy (PDT) with 5-ALA as photosensitizer using human muscle cancer cells as experimental model. We study the photosensitizer uptake, cytotoxicity, phototoxicity, and cellular viability of the RD cells which was estimated by means of neutral-red spectrophotometric assay. The given experiment was consisted of two steps. For the first one, RD cells were exposed to 5-ALA at concentrations of 0 up to 1000 μg of ALA/ml in minimum essential medium (MEM). The optimal uptake of photosensitizer (5-ALA) in RD cells was investigated by means of spectrometric measurements. Cells viability was determined by means of neutral red assay (NRA). In the second step, 5-ALA exposed RD cells were irradiated with red light (a diode laser, λ = 635 nm) at total light dose of 80 J/cm 2 . The influence of different incubation times and concentrations of 5-ALA, different irradiation doses and various combinations of photosensitizer and light doses on the viability of RD cells were investigated. It was observed that sensitizer concentration or light doses have no significant effect on cells viability when studied independently. The maximal cellular uptake occurred after 47 hours in vitro incubation. The phototoxic assay showed that ALA-PDT induced killing of 76% of the cells at 250 μg/ml drug dose and 80 J/cm 2 light dose.

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Section: Resultssupporting

confidence: 94%

Section: Ala Cytotoxicity and Phototoxicity Testsmentioning

confidence: 99%

Study of the efficacy of 5-ALA mediated photodynamic therapy on human rhabdomyosarcoma cell line (RD)

Fakhar-e-Alam¹,

Firdous²,

Zaidi³

et al. 2010

Laser Phys. Lett.

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“…Higher absorption imply in higher production of singlet oxygen and therefore higher photodynamic action and better PDT outcome. This study is in agreement with earlier studies using a variety of photosensitizers in different chemical and biological environments [38][39][40][41][42].…”

Section: Discussionsupporting

confidence: 93%

Analysis of the combined effect of lasers of different wavelengths for PDT outcome using 600, 630, and 660 nm

et al. 2011

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Abstract:We investigated the effects of photodynamic therapy (PDT) outcome when combining three laser systems that produce light in three different wavelengths (600, 630, and 660 nm). Cooperative as well as independent effects can be observed. We compared the results of the combined wavelengths of light with the effect of single laser for the excitation of the photosensitizer. In the current experiment, the used photosensitizer was Photogem R (1.5 mg/kg). Combining two wavelengths for PDT, their cumulative dose and different penetrability may change the overall effect of the fluence of light, which can be effective for increasing the depth of necrosis. This evaluation was performed by comparing the depth and specific aspect of necrosis obtained by using single and dual wavelengths for irradiation of healthy liver of male Wistar rats. We used 15 animals and divided them in five groups of three animals. First, Photogem R was administered; follow by measurement of the fluorescence spectrum of the liver before PDT to confirm the level of accumulation of photosensitizer in the tissue. After that, an area of 1 cm 2 of the liver was illuminated using different laser combinations. Qualitative analysis of the necrosis was carried out through histological and morphological study.

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“…In addition to photochemical damaging, some enhanced thermal damaging is expected because of the more effective laser heating of selectively stained tissue sites. Apoptosis and other morphological modifications of cells are typically detected using histological analysis of samples [23][24][25][26][27][28][29][31][32][33]. In our work, we used machine vision based inspection of the morphological changes of adipocytes.…”

Section: Methodsmentioning

confidence: 99%

Optical image analysis of fat cells for indocyanine green mediated near-infrared laser treatment

et al. 2011

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Fat tissue engineering by near-infrared laser treatment is investigated in vitro. For the quantitative estimation of efficiency of treatment and consequential processes on the cellular level, a computational method for optical image analysis is proposed. The method is based on the fractal dimension data analysis of fat cell structure. The conducted experiments confirm the feasibility of the proposed method to obtain quantitative information of the cellular processes. The results indicate that the proposed method is promising for estimating spatial changes of biological structure. CCD cameraThermostatic Experimental setup consists of a CCD camera, thermostatic table, sample holder, and CW diode laser

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In vitrostudy of 5-aminolevulinic acid-based photodynamic therapy for apoptosis in human cervical HeLa cell line

Cited by 68 publications

References 21 publications

Study of the efficacy of 5-ALA mediated photodynamic therapy on human rhabdomyosarcoma cell line (RD)

Study of the efficacy of 5-ALA mediated photodynamic therapy on human rhabdomyosarcoma cell line (RD)

Analysis of the combined effect of lasers of different wavelengths for PDT outcome using 600, 630, and 660 nm

Optical image analysis of fat cells for indocyanine green mediated near-infrared laser treatment

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