Optical image analysis of fat cells for indocyanine green mediated near-infrared laser treatment

Yanina, Irina Yu.; Bochko, Vladimir; Alander, Jarmo T.; Tuchin, Valery V.

doi:10.1002/lapl.201110048

Cited by 11 publications

(13 citation statements)

References 51 publications

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“…Photodynamic therapy (PDT) has emerged as a promising therapeutic modality for the treatment of oncological diseases [1,2]. It has the advantage of dual selectivity, due to the preferential localization of the photosensitizer by the malignant tissue and restriction of photoactivation to the tumor site due to localized light irradiation [3,4].…”

Section: Introductionmentioning

confidence: 99%

“…Manipulat ing light flounce rate, timing of illumination following photosensitizer administration or a combination of these it is possible to modulate the role played by each of these factors in the final damage towards the tumor. Studies from in vitro culture systems and in vivo ani mal models have indicated that both necrosis and apo 1 The article is published in the original. ptosis of the target cells or tissues represent the major therapeutic effect of PDT [11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

et al. 2012

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Photodynamic therapy (PDT) is a kind of photochemo therapeutic treatment that exerts its effect mainly through the induction of cell death. Distinct types of cell death may be elicited by different PDT regimes. In this study, efforts are underway to optimize PDT protocols for improved efficacy and combination of all three PDT mechanisms involved in the different human carcinomas cell narcosis. Our in vitro cell cul ture experiments with 5 aminolevulanic acid (ALA) a clinically approved photiosensitizer (PS) and 635 nm laser light have yielded promising results, as follow: (1) (human cervical cancer (HeLa) cell line incubated, for 18 h, with 30 µg/ml of 5 ALA, treated with laser light dose of 50 J/cm 2 can produce 85% of cell killing (2) human larynx carcinoma (Hep2c) cell line incubated, for 7 h, with 55 µg/ml of 5 ALA, treated with laser light dose of 85 J/cm 2 can produce 75% of cell killing (3) human liver cancer (HepG2) cell line incubated, for 22-48 h, with 262 µg/ml of 5 ALA, treated with laser light dose of 120 J/cm 2 can produce 95% of cell killing (4) human muscle cancer (RD) cell line incubated, for 47 h, with 250 µg/ml of 5 ALA, treated with laser light dose of 80 J/cm 2 can produce 76% of cell killing (5) Human embryonic kidney (HEK293T) cell line incu bated, for 18 h, with 400 µg/ml of 5 ALA, treated with laser light dose of 40 J/cm 2 can produce 82% of cell killing confirming the efficacy of photodynamic therapy.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

et al. 2012

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“…Besides experiences from new laser systems for practi cal use [29], recent imaging techniques [30,31] can improve future investigations of the dosimetry of light in ophthalmology. Fig.…”

Section: Discussionmentioning

confidence: 99%

Comparison of retina damage thresholds simulating the femtosecond-laser in situ keratomileusis (fs-LASIK) process with two laser systems in the CW- and fs-regime

Sander¹,

Minet

Zabaryło

et al. 2012

Laser Phys.

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The femtosecond laser in situ keratomileusis procedure affords the opportunity to correct ametropia by cutting transparent corneal tissue with ultra short laser pulses. Thereby the tissue cut is gener ated by a laser induced optical breakdown in the cornea with ultra short laser pulses in the near infrared range. Compared to standard procedures such as photorefractive keratectomy and laser in situ keratomileusis with the excimer laser, where the risk potential for the eye is low due to the complete absorption of ultraviolet irradiation from corneal tissue, only a certain amount of the pulse energy is deposited in the cornea during the fs LASIK process. The remaining energy propagates through the eye and interacts with the retina and the strong absorbing tissue layers behind. The objective of the presented study was to determine and compare the retina damage thresholds during the fs LASIK process simulated with two various laser systems in the CW and fs regime.

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“…The use of these terms reflects the specificity of biochemical and morphological approaches . Depending on technology applied, cell apoptosis or necrosis may take place . Another method of fat tissue destruction, namely photodynamic/photothermal method, may provide reduction of regional or site‐specific accumulations of adipose tissue least‐invasively .…”

Section: Introductionmentioning

confidence: 99%

Skin and subcutaneous fat morphology alterations under the LED or laser treatment in rats in vivo

Yanina

Navolokin

Bucharskaya

et al. 2019

Journal of Biophotonics

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The main objective of this work is to quantify the impact of photodynamic/photothermal treatment by using visible LED and NIR laser irradiation through the skin of subcutaneous fat in vivo followed up by tissue sampling and histology. The optical method may provide reduction of regional or site‐specific accumulations of abdominal or subcutaneous adipose tissue precisely and least‐invasively by inducing cell apoptosis and controlled necrosis of fat tissue. As photodynamic/photothermal adipose tissue sensitizers Brilliant Green (BG) or Indocyanine Green (ICG) dyes were injected subcutaneously in rats. The CW LED device (625 nm) or CW diode laser (808 nm) were used as light sources, respectively. Biopsies of skin together with subcutaneous tissues were taken for histology. The combined action BG‐staining and LED‐irradiation (BG + LED) or ICG‐staining and NIR‐laser irradiation (ICG + NIR) causes pronounced signs of damage of adipose tissue characterized by a strong stretching, thinning, folding and undulating of cell membranes and appearance of necrotic areas. As a posttreatment after 14 days only connective tissue was observed at the site of necrotic areas. The data obtained are important for safe light treatment of site‐specific fat accumulations, including cellulite. This work provides a basis for the development of fat lipolysis technologies and to move them to clinical applications. Schematics of animal experiment.

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Optical image analysis of fat cells for indocyanine green mediated near-infrared laser treatment

Cited by 11 publications

References 51 publications

In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

Comparison of retina damage thresholds simulating the femtosecond-laser in situ keratomileusis (fs-LASIK) process with two laser systems in the CW- and fs-regime

Skin and subcutaneous fat morphology alterations under the LED or laser treatment in rats in vivo

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