Ultrastructural localization of neuronal nitric oxide synthase-immunoreactivity in the rat ileum

Matini, Patrizia; Faussone–Pellegrini, Maria Simonetta

doi:10.1016/s0304-3940(97)00414-x

Cited by 36 publications

(23 citation statements)

References 15 publications

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“…7). It has been reported that NOS-immunoreactive nerve endings are close to ICC-DMP in the rat ileum (27) and to ICC-IM in the mouse proximal colon (28). A moderate decrease in immunopositive cells to antinNOS antibody was observed in the myenteric plexus of the mutant mice (Fig.…”

Section: Discussionmentioning

confidence: 69%

Essential Role of the Interstitial Cells of Cajal in Nitric Oxide-Mediated Relaxation of Longitudinal Muscle of the Mouse Ileum

et al. 2004

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Abstract. The role of interstitial cells of Cajal (ICC) in electrical field stimulation (EFS)-induced neurogenic responses in ileum was studied by using the ICC-deficient mutant (SLC-W / W V ) mouse and its wild type. In the immunohistochemical study with anti-c-Kit antibody, ICC was observed in the myenteric plexus (MY) and deep muscular plexus (DMP) region in the wild type. In the mutant, ICC-MY were lost, only ICC-DMP were present. EFS induced a rapid contraction of the ileal segments from the wild type mouse in the direction of longitudinal muscle. In the mutant mouse, onset of contraction was delayed and its rate was slowed. EFS induced nonadrenergic, noncholinergic (NANC) relaxation in the presence of atropine and guanethidine in the wild type. A nitric oxide synthase inhibitor inhibited the relaxation and L-arginine reversed it. In the mutant, EFS did not induce NANC relaxation. There was no difference between the responsiveness of the segments from wild type and mutant mice to exogenously added acetylcholine or Nor-1. Taking into account the selective loss of ICC-MY in the mutant mice, it seems likely that ICC-MY have an essential role in inducing nitric oxidemediated relaxation of longitudinal muscle of the mouse ileum and that ICC-MY partly participate in EFS-induced contraction.

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Section: Discussionmentioning

confidence: 69%

Essential Role of the Interstitial Cells of Cajal in Nitric Oxide-Mediated Relaxation of Longitudinal Muscle of the Mouse Ileum

et al. 2004

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“…The colocalization in these same cells of the NADPH-diaphorase and constitutive NOS was shown with antibodies to eNOS and nNOS (Xue et al, 1994). Matini and Faussone-Pellegrini (1997) found by combined LM and TEM the presence of nNOS-IR in ICC-MGP of rat ileum. ICC in LES and pyloric sphincter of the mouse were also NOSϩ (Ward et al, 1998).…”

Section: Osmic Acid/zinc Iodide (Champy-maillet Method)mentioning

confidence: 72%

“…A pacemaker function (slow wave generation) has so far been demonstrated in two of these subsets, namely the ICC located in between the longitudinal and circular muscle layers and associated with the myenteric (Auerbach's) ganglionated plexus (named ICC-MGP or ICC-AP), and the colonic ICC associated with the submuscular plexus (named ICC-SMP). A role as mediator/ modulator of circular muscle inhibitory innervation (Daniel and Posey-Daniel, 1984) is strongly supported by recent studies in stomach (Burns et al, 1996;Ward et al, 1998) for the ICC located in the interstices of the circular muscle layer (named ICC-CM) and in small intestine (Matini and Faussone-Pellegrini, 1997) for the ICC (named ICC-DMP) located at the deep muscular plexus site between the inner and outer portions of the circular muscle layer. Recently a role in excitatory innervation has also been proposed for the ileal ICC (Grady et al, 1996;Portbury et al, 1996;Vannucchi et al, 1997).…”

Section: Interstitial Cells Of Cajal (Icc)mentioning

confidence: 87%

Guide to the identification of interstitial cells of Cajal

Faussone–Pellegrini

Thuneberg

1999

Microsc. Res. Tech.

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144

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“…Several neurotransmitters have been identified in these nerve endings by immunohistochemistry, both under light and electron microscope [16,[20][21][22][23][24][25][26] [14][15][16][17][18][27][28][29][30] and functional studies confirmed that these cells are one of their targets [30][31][32][33]. No information is available on a possible sensory innervation of these cells, but this possibility cannot be excluded and deserves further attention.…”

Section: Icc Innervationmentioning

confidence: 99%

Relationships between neurokinin receptor‐expressing interstitial cells of Cajal and tachykininergic nerves in the gut

Faussone–Pellegrini

2006

J Cellular Molecular Medi

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The so-called interstitial cells of Cajal (ICC) are distributed throughout the muscle coat of the alimentary tract with characteristic intramural location and species-variations in structure and staining. Several ICC sub-types have been identified: ICC-DMP, ICC-MP, ICC-IM, ICC-SM. Gut motility is regulated by ICC and each sub-type is responsible for the electrical activities typical of each gut region and/or muscle layer. The interstitial position of the ICC between nerve endings and smooth muscle cells has been extensively considered. Some of these nerve endings contain tachykinins. Three distinct tachykinin receptors (NK1r, NK2r and NK3r) have been demonstrated by molecular biology. Each of them binds with different affinities to a series of tachykinins (SP, NKA and NKB). In the ileum, SP-immunoreactive (SP-IR) nerve fibers form a rich plexus at the deep muscular plexus (DMP), distributed around SP-negative cells, and ICC-DMP intensely express the SP-preferred receptor NK1r; conversely a faint NK1r-IR is detected on the ICC-MP and mainly after receptor internalization was induced by agonists. ICC-IM are never stained in laboratory mammals, while those of the human antrum are NK1r-IR. RT-PCR conducted on isolated ileal ICC-MP and gastric ICC-IM showed that these cells express NK1r and NK3r. Colonic ICC, except those in humans, do not express NK1r-IR, at least in resting conditions. Outside the gut, NK1r-IR cells were seen in the arterial wall and exocrine pancreas. In the mouse gut only, NK1r-IR is present in non-neuronal cells located within the intestinal villi, so-called myoid cells, which are c-kit-negative and α-smooth muscle actin-positive. Immunohistochemistry and functional studies confirmed that ICC receive input from SP-IR terminals, with differences between ICC sub-types. In the rat, very early after birth, NK1r is expressed by the ICC-DMP and SP by the related nerve varicosities. Studies on pathological conditions are few and those on mutant strains practically absent. It has only been reported that in the inflamed ileum of rats the NK1r-IR ICC-DMP disappear and that at the peak of inflammatory conditions ICC-MP are NK1r-IR. In the ileum of mice with a mutation in the W locus, ICC-DMP were seen to express c-kit-IR but not NK1-IR, and SP-IR innervation seems unchanged. In summary, there are distinct ICC populations, each of them under a different tachykininergic control and, likely, having different functions. Further studies are recommended at the aim of understanding ICC involvement in modulating/transmitting tachykininergic inputs.

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Ultrastructural localization of neuronal nitric oxide synthase-immunoreactivity in the rat ileum

Cited by 36 publications

References 15 publications

Essential Role of the Interstitial Cells of Cajal in Nitric Oxide-Mediated Relaxation of Longitudinal Muscle of the Mouse Ileum

Essential Role of the Interstitial Cells of Cajal in Nitric Oxide-Mediated Relaxation of Longitudinal Muscle of the Mouse Ileum

Guide to the identification of interstitial cells of Cajal

Relationships between neurokinin receptor‐expressing interstitial cells of Cajal and tachykininergic nerves in the gut

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