Umbelliferon, Glutamat Eksitotoksisitesine Karşı Primer Kortikal Nöron Hücrelerini Korur mu?

Demirkaya, Alper Kürşat; Gündoğdu, Gülşah; Karakaya, Songül; Taşci, Şeymanur Yilmaz; Nalcı, Kemal Alp; Hacımüftüoğlu, Ahmet

doi:10.9775/kvfd.2021.25439

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To validate that the recorded signals were spontaneous spikes from NoNs, we administered 20 uM glutamate into the culture medium during the recording process on day 20. Glutamate is a major excitatory neurotransmitter in the central nervous system that can enhance the neural activities ( DEMİRKAYA et al, 2021 ). Figure 5H illustrates the response of NoNs (depicted by blue dots) and single neurons (depicted by orange dots) to glutamate.…”

Section: Resultsmentioning

confidence: 99%

Single neurons on microelectrode array chip: manipulation and analyses

Zhang,

Wang,

Huang

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Chips-based platforms intended for single-cell manipulation are considered powerful tools to analyze intercellular interactions and cellular functions. Although the conventional cell co-culture models could investigate cell communication to some extent, the role of a single cell requires further analysis. In this study, a precise intercellular interaction model was built using a microelectrode array [microelectrode array (MEA)]-based and dielectrophoresis-driven single-cell manipulation chip. The integrated platform enabled precise manipulation of single cells, which were either trapped on or transferred between electrodes. Each electrode was controlled independently to record the corresponding cellular electrophysiology. Multiple parameters were explored to investigate their effects on cell manipulation including the diameter and depth of microwells, the geometry of cells, and the voltage amplitude of the control signal. Under the optimized microenvironment, the chip was further evaluated using 293T and neural cells to investigate the influence of electric field on cells. An examination of the inappropriate use of electric fields on cells revealed the occurrence of oncosis. In the end of the study, electrophysiology of single neurons and network of neurons, both differentiated from human induced pluripotent stem cells (iPSC), was recorded and compared to demonstrate the functionality of the chip. The obtained preliminary results extended the nature growing model to the controllable level, satisfying the expectation of introducing more elaborated intercellular interaction models.

show abstract

Section: Resultsmentioning

confidence: 99%

Single neurons on microelectrode array chip: manipulation and analyses

Zhang,

Wang,

Huang

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

A review of the ethnomedicinal, phytochemical, and pharmacological properties of the Ferulago genus based on Structure–Activity Relationship (SAR) of coumarins

Dabaghian,

Aalinezhad,

Kesheh

et al. 2024

DARU J Pharm Sci

View full text Add to dashboard Cite

Thunbergia laurifolia Leaf Extract Inhibits Glutamate-Induced Neurotoxicity and Cell Death through Mitophagy Signaling

et al. 2021

View full text Add to dashboard Cite

Oxidative stress plays a crucial role in neurodegeneration. Therefore, reducing oxidative stress in the brain is an important strategy to prevent neurodegenerative disorders. Thunbergia laurifolia (Rang-jued) is well known as an herbal tea in Thailand. Here, we aimed to determine the protective effects of T. laurifolia leaf extract (TLE) on glutamate-induced oxidative stress toxicity and mitophagy-mediated cell death in mouse hippocampal cells (HT-22). Our results reveal that TLE possesses a high level of bioactive antioxidants by LC–MS technique. We found that the pre-treatment of cells with TLE prevented glutamate-induced neuronal death in a concentration-dependent manner. TLE reduced the intracellular ROS and maintained the mitochondrial membrane potential caused by glutamate. Moreover, TLE upregulated the gene expression of antioxidant enzymes (SOD1, SOD2, CAT, and GPx). Interestingly, glutamate also induced the activation of the mitophagy process. However, TLE could reverse this activity by inhibiting autophagic protein (LC3B-II/LC3B-I) activation and increasing a specific mitochondrial protein (TOM20). Our results suggest that excessive glutamate can cause neuronal death through mitophagy-mediated cell death signaling in HT-22 cells. Our findings indicate that TLE protects cells from neuronal death by stimulating the endogenous antioxidant enzymes and inhibiting glutamate-induced oxidative toxicity via the mitophagy–autophagy pathway. TLE might have potential as an alternative or therapeutic approach in neurodegenerative diseases.

show abstract

Umbelliferon, Glutamat Eksitotoksisitesine Karşı Primer Kortikal Nöron Hücrelerini Korur mu?

Cited by 3 publications

References 0 publications

Single neurons on microelectrode array chip: manipulation and analyses

Single neurons on microelectrode array chip: manipulation and analyses

A review of the ethnomedicinal, phytochemical, and pharmacological properties of the Ferulago genus based on Structure–Activity Relationship (SAR) of coumarins

Thunbergia laurifolia Leaf Extract Inhibits Glutamate-Induced Neurotoxicity and Cell Death through Mitophagy Signaling

Contact Info

Product

Resources

About