Understanding Cultivar-Specificity and Soil Determinants of the Cannabis Microbiome

Winston, Max; Hampton-Marcell, Jarrad; Zarraonaindia, Iratxe; Owens, Sarah M.; Moreau, Corrie S.; Gilbert, Jack A.; Hartsel, Joshua A.; Kennedy, Suzanne; Gibbons, Sean M.

doi:10.1371/journal.pone.0099641

Cited by 71 publications

(60 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Winston et al (2014), understanding the relationship between microbes and Cannabis, a medicinally and economically important crop, can affect its agricultural practices, improving the ability of the plant to grow. They presented the first description of the endorhiza-, rhizosphere-, and bulk soil-associated microbiome of five distinct Cannabis cultivars using 16S rRNA gene sequencing.…”

Section: Discussionmentioning

confidence: 99%

Rhizosphere bacteriome of the medicinal plant Sapindus saponaria L. revealed by pyrosequencing

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. Sapindus saponaria L. of Sapindaceae family is popularly known as soldier soap and is found in Central and South America. A study of such medicinal plants might reveal a more complex diversity of microorganisms as compared to non-medicinal plants, considering their metabolic potential and the chemical communication between their natural microbiota. Rhizosphere is a highly diverse microbial habitat with respect to both the diversity of species and the size of the community. Rhizosphere bacteriome associated with medicinal plant S. saponaria is still poorly known. The objective of this study was to assess the rhizosphere microbiome of the medicinal plant S. saponaria using pyrosequencing, a cultureindependent approach that is increasingly being used to estimate the number of bacterial species present in different environments. In their rhizosphere microbiome, 26 phyla were identified from 5089 sequences of 16S rRNA gene, with a predominance of Actinobacteria (33.54%), Acidobacteria (22.62%), and Proteobacteria (24.72%). The rarefaction curve showed a linear increase, with 2660 operational taxonomic units at 3% distance sequence dissimilarity, indicating that the rhizosphere microbiome associated with S. saponaria was highly diverse with groups of bacteria important for soil management, which could be further exploited for agricultural and biotechnological purposes.

show abstract

Section: Discussionmentioning

confidence: 99%

Rhizosphere bacteriome of the medicinal plant Sapindus saponaria L. revealed by pyrosequencing

et al. 2016

View full text Add to dashboard Cite

show abstract

“…While epiphytic microbes may originate from many sources like aerosols, dusts and liquids, or via human contact, endophytes typically gain entry from the rhizosphere via root junctions, and subsequent translocation through the xylem 4, 5 . Considering this and the known impact that the soil and root microbiome has on plant growth and development 6, 7 , all sources of microbial inputs, including below ground compartments should be considered important for optimal Cannabis growth and consumer safety 8 .…”

Section: Introductionmentioning

confidence: 99%

Metagenomic analysis of medicinal Cannabis samples; pathogenic bacteria, toxigenic fungi, and beneficial microbes grow in culture-based yeast and mold tests

et al. 2016

View full text Add to dashboard Cite

Background: The presence of bacteria and fungi in medicinal or recreational Cannabis poses a potential threat to consumers if those microbes include pathogenic or toxigenic species. This study evaluated two widely used culture-based platforms for total yeast and mold (TYM) testing marketed by 3M Corporation and Biomérieux, in comparison with a quantitative PCR (qPCR) approach marketed by Medicinal Genomics Corporation. Methods: A set of 15 medicinal Cannabis samples were analyzed using 3M and Biomérieux culture-based platforms and by qPCR to quantify microbial DNA. All samples were then subjected to next-generation sequencing and metagenomics analysis to enumerate the bacteria and fungi present before and after growth on culture-based media. Results: Several pathogenic or toxigenic bacterial and fungal species were identified in proportions of >5% of classified reads on the samples, including Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, Ralstonia pickettii, Salmonella enterica, Stenotrophomonas maltophilia, Aspergillus ostianus, Aspergillus sydowii, Penicillium citrinum and Penicillium steckii. Samples subjected to culture showed substantial shifts in the number and diversity of species present, including the failure of Aspergillus species to grow well on either platform. Substantial growth of Clostridium botulinum and other bacteria were frequently observed on one or both of the culture-based TYM platforms. The presence of plant growth promoting (beneficial) fungal species further influenced the differential growth of species in the microbiome of each sample. Conclusions: These findings have important implications for the Cannabis and food safety testing industries.

show abstract

“…Medicago truncatula when subjected to salinity stress [42], and also has been found in the endosphere of cannabis, potato and banana [43]- [45]. Other species enriched in both endospheres were P.…”

Section: Amplicon-based Taxonomic Profilingmentioning

confidence: 96%

Isolation and Studies on Bacterial Endophytes from Two Venezuelan Rice Cultivars

Moronta-Barrios¹,

Gionechetti²,

Pallavicini³

et al. 2017

Preprint

View full text Add to dashboard Cite

Abstract:Rice is currently the most important food crop in the world and we are only just beginning to study the bacterial associated microbiome. It is of importance to perform screenings of the core rice microbiota and also to develop new plant-microbe models and simplified communities for increasing our understanding about the formation and function of its microbiome. In order to begin to address this aspect, we have performed the isolation of hundreds bacterial isolates obtained from endorhizosphere of two rice cultivars from Venezuela. The validation of plant-growth promoting bacterial activities in vitro has led us to select and characterize 15 isolates for in planta studies such as germination test, endophytism ability and plant growth promotion. Consequently, a set of 10 isolates was selected for the set-up of an endophytic consortium as a simplified model of the natural rice bacterial endomicrobiota. Upon inoculation, the colonization and abundance of each strain within the rice roots was tracked by a culture-independent technique in gnotobiotic conditions in a 30 days period. Four strains belonging to Pseudomonas, Agrobacterium and Delftia genera have shown a promising capacity for colonizing and coexistence in root tissues. On the other hand, a bacterial community taxonomic profiling of the rhizosphere and the endorhizosphere of both cultivars were obtained and are discussed. This study is part of a growing body of research on core crops microbiome and simplified microbiomes, which strengthens the formation process of the endophytic community leading to a better understanding of the rice microbiome.

show abstract

Understanding Cultivar-Specificity and Soil Determinants of the Cannabis Microbiome

Cited by 71 publications

References 48 publications

Rhizosphere bacteriome of the medicinal plant Sapindus saponaria L. revealed by pyrosequencing

Rhizosphere bacteriome of the medicinal plant Sapindus saponaria L. revealed by pyrosequencing

Metagenomic analysis of medicinal Cannabis samples; pathogenic bacteria, toxigenic fungi, and beneficial microbes grow in culture-based yeast and mold tests

Isolation and Studies on Bacterial Endophytes from Two Venezuelan Rice Cultivars

Contact Info

Product

Resources

About