Unique organization of the 16S–23S intergenic spacer regions of strains of Acinetobacter baylyi provides a means for its identification from other Acinetobacter species

Clinical Carbapenem-Resistant Acinetobacter baylyi Strain Coharboring bla SIM-1 and bla OXA-23 from China

“…EU042163) and A. baylyi ADP1 (GenBank accession no. CR543861); they were less than 85% identical to those of the other genomic species, which indicated that NB09A30 is an A. baylyi strain (12). PCR was performed to check for the presence of carbapenem-hydrolyzing class D ␤-lactamase (CHDL) genes (bla OXA-23 , bla OXA-24 , and bla OXA-58 ), MBL genes (bla IMPtype and bla VIM -type genes, bla SIM-1 , bla SPM-1 , bla GIM-1 , and bla NDM-1 ), and the bla KPC -type gene, as previous reported (13,16).…”

“…The 16S-23S rRNA intergenic spacer region (ISR) of NB09A30 was amplified and cloned into the pGEM-T Easy vector for sequencing (5). This showed that NB09A30 possessed ISRs with different lengths (long ISR, 650 bp; short ISR, 611 bp), a unique organization of the 16S-23S rRNA ISRs of A. baylyi (12). The ISR sequences were 94% identical to those of A. baylyi strain 93A2 (GenBank accession no.…”

Unusual features of the sequences of copies of the 16S–23S rRNA internal transcribed spacer regions of Acinetobacter bereziniae, Acinetobacter guillouiae and Acinetobacter baylyi arise from horizontal gene transfer events

“…As both 16S rRNA (unpublished data) and ITS sequence data (see later) are consistent with BENAB7 being a member of A. bereziniae, this strain is referred to subsequently as A. bereziniae BENAB7. The A. baylyi strains used in this study were those detailed by Maslunka et al (2008).…”

“…It is known that each Acinetobacter species may contain between three and seven rrn operons (Maslunka et al, 2006), and this is important because one or more of the individual internal 16S-23S rRNA internal transcribed spacer region (ITS) copies can vary in its length and, hence, sequence between rrn operons. For example, Acinetobacter baylyi strains ADP1/B2 (Barbe et al, 2004;Carr et al, 2004) each contain ITS copies of two lengths, which are quite different to the two copies in A. baylyi strain 93A2 (Maslunka et al, 2008). Chang et al (2005), using agarose gel electrophoresis of ITS PCR amplicons, identified a further seven species with variable length ITS copies.…”

Isolation and molecular identification of polyaromatic hydrocarbons- utilizing bacteria from crude petroleum oil samples

“…Isolation and characterization of strains belonging to Acinetobacter and Klebsiella that can grow on crude oil were reported by many researchers. In most of the cases, these bacterial species have been identified throughout sequencing of the 16S rDNA or 16S-23S intergenic spacer regions (Rainey et al, 1994;Maslunka et al, 2008;Guo et al, 2010;Nemec et al, 2011).…”